Perioperative Medicine  |   January 2018
Effects of Hypercapnia on Acute Cellular Rejection after Lung Transplantation in Rats
Author Notes
  • From the Department of Anesthesiology (Hei Long Jiang Province Key Lab of Research on Anesthesiology and Critical Care Medicine), Second Affiliated Hospital of Harbin Medical University, Harbin, China (J.T., Y.L., T.J., C.Z., D.S., X.C.); and the Department of Anesthesiology, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China (L.W.).
  • Submitted for publication December 5, 2016. Accepted for publication September 7, 2017.
    Submitted for publication December 5, 2016. Accepted for publication September 7, 2017.×
  • Address correspondence to Dr. Cui: Second Affiliated Hospital of Harbin Medical University, No. 246, XueFu Road, NanGang District, Harbin 150081, Heilongjiang Province, China. cuixiaoguang1018@126.com. Information on purchasing reprints may be found at www.anesthesiology.org or on the masthead page at the beginning of this issue. Anesthesiology’s articles are made freely accessible to all readers, for personal use only, 6 months from the cover date of the issue.
Article Information
Perioperative Medicine / Basic Science / Cardiovascular Anesthesia / Respiratory System / Thoracic Anesthesia
Perioperative Medicine   |   January 2018
Effects of Hypercapnia on Acute Cellular Rejection after Lung Transplantation in Rats
Anesthesiology 1 2018, Vol.128, 130-139. doi:10.1097/ALN.0000000000001908
Anesthesiology 1 2018, Vol.128, 130-139. doi:10.1097/ALN.0000000000001908
Abstract

Background: Hypercapnia alleviates pulmonary ischemia–reperfusion injury, regulates T lymphocytes, and inhibits immune reaction. This study aimed to evaluate the effect of hypercapnia on acute cellular rejection in a rat lung transplantation model.

Methods: Recipient rats in sham-operated (Wistar), isograft (Wistar to Wistar), and allograft (Sprague–Dawley to Wistar) groups were ventilated with 50% oxygen, whereas rats in the hypercapnia (Sprague–Dawley to Wistar) group were administered 50% oxygen and 8% carbon dioxide for 90 min during reperfusion (n = 8). Recipients were euthanized 7 days after transplantation.

Results: The hypercapnia group showed a higher oxygenation index (413 ± 78 vs. 223 ± 24), lower wet weight-to-dry weight ratio (4.23 ± 0.54 vs. 7.04 ± 0.80), lower rejection scores (2 ± 1 vs. 4 ± 1), and lower apoptosis index (31 ± 6 vs. 57 ± 4) as compared with the allograft group. The hypercapnia group showed lower CD8 (17 ± 4 vs. 31 ± 3) and CD68 (24 ± 3 vs. 43 ± 2), lower CD8+ T cells (12 ± 2 vs. 35 ± 6), and higher CD4/CD8 ratio (2.2 ± 0.6 vs. 1.1 ± 0.4) compared to the allograft group. Tumor necrosis factor-α (208 ± 40 vs. 292 ± 49), interleukin-2 (30.6 ± 6.7 vs. 52.7 ± 8.3), and interferon-γ (28.1 ± 4.9 vs. 62.7 ± 10.1) levels in the hypercapnia group were lower than those in allograft group. CD4, CD4+ T cells, and interleukin-10 levels were similar between groups.

Conclusions: Hypercapnia ameliorated acute cellular rejection in a rat lung transplantation model.