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Meeting Abstracts  |   July 1997
Positive Inotropic and Lusitropic Effects of Triiodothyronine in Conscious Dogs with Pacing-induced Cardiomyopathy 
Author Notes
  • (Jamali) Fellow in Medicine, Division of Cardiovascular Diseases.
  • (Pagel) Associate Professor of Anesthesiology.
  • (Hettrick) Assistant Professor of Anesthesiology.
  • (Lowe) Research Fellow in Anesthesiology.
  • (Tessmer) Senior Research Scientist.
  • (Warltier) Professor of Anesthesiology, Pharmacology and Toxicology, and Medicine (Division of Cardiovascular Diseases) and Vice Chairman for Research of Anesthesiology.
  • Received from the Departments of Medicine (Division of Cardiovascular Diseases), Anesthesiology, and Pharmacology and Toxicology, the Medical College of Wisconsin and the Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin. Submitted for publication November 21, 1996. Accepted for publication March 4, 1997. This work was supported by a FAER Young Investigator Award (JRK), US PHS grant HL 54820 (DCW), and Anesthesiology Research Training Grant GM 08377 (DCW).
  • Address reprint requests to Dr. Warltier: Medical College of Wisconsin, MEB, Room 462C, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226.
Article Information
Meeting Abstracts   |   July 1997
Positive Inotropic and Lusitropic Effects of Triiodothyronine in Conscious Dogs with Pacing-induced Cardiomyopathy 
Anesthesiology 7 1997, Vol.87, 102-109. doi:
Anesthesiology 7 1997, Vol.87, 102-109. doi:
Recognition that abnormalities in thyroid hormone metabolism may occur in patients with congestive heart failure has stimulated research attempting to define the role of thyroid hormone therapy in this clinical setting. A major finding in heart failure is the reduction of free triiodothyronine (T3) levels. Reversal of such decreases in free T3concentration via the administration of exogenous T3has been proposed to increase left ventricular function and improve the short-term prognosis of patients with heart failure. [1 ] Several investigations in experimental animals and humans have suggested the feasibility and usefulness of this therapeutic approach. T3has been shown to enhance the functional recovery of stunned myocardium [2–4 ] and to improve contractility in models of myocardial infarction and ventricular remodeling. [5,6 ] T3also produced beneficial hemodynamic effects in patients with acute myocardial infarction, [7,8 ] after sudden cardiac death, [9 ] and during [10 ] and after cardiopulmonary bypass. [11 ] Thyroid hormone therapy has recently been shown to improve functional capacity in patients with idiopathic dilated cardiomyopathy. [12 ] However, the influence of T3on systemic hemodynamics and invasively derived indices of left ventricular systolic and diastolic function in the cardiomyopathic heart have not been described.
The present investigation tested the hypothesis that T3enhances myocardial contractility, improves left ventricular isovolumic relaxation, and produces beneficial hemodynamic effects in conscious, unsedated dogs with rapid ventricular pacing-induced cardiomyopathy. This experimental model produces time-dependent bi-ventricular dilation, increases in left and right ventricular filling pressures, [13,14 ] abnormalities in intracellular calcium (Ca2+) homeostasis, [15 ] left ventricular systolic and diastolic dysfunction, [16,17 ] and derangements in thyroid hormone metabolism [18 ] similar to those found in patients with dilated congestive cardiomyopathy. [19 ] Pacing-induced cardiomyopathy is relatively easy to produce in dogs [13,14 ] and provides a suitable model to study the cardiovascular effects of T3in the setting of severe left ventricular dysfunction.
Materials and Methods 
All experimental procedures and protocols used in this investigation were reviewed and approved by the Animal Care Committee of the Medical College of Wisconsin. All procedures conformed to the Guiding Principles in the Care and Use of Animals of the American Physiologic Society and were performed according to the Guide for the Care and Use of Laboratory Animals (Department of Health, Education, and Welfare-Department of Health and Human Services publication [NIH] 85–23, revised 1996).
Surgical Preparation 
The surgical implantation of instruments has been previously described in detail. [20 ] Briefly, during general anesthesia and using aseptic techniques, a left thoracotomy was performed in conditioned mongrel dogs for placement of instruments for measurement of aortic, left atrial, and intrathoracic pressures (heparin-filled catheters), subendocardial segment length (ultrasonic crystals), and relative cardiac output (ascending thoracic aortic ultrasonic flow transducer). A miniature micromanometer was placed in the left ventricle for measurement of continuous left ventricular pressure and the peak rate of increase of left ventricular pressure (dP/dtmax). A hydraulic vascular occluder was placed around the inferior vena cava for abrupt alteration of left ventricular preload used to generate left ventricular pressure-segment length diagrams. Stainless steel pacing electrodes were sutured to the epicardial surface of the left ventricular free wall and attached to an external programmable pacemaker. All instruments were firmly secured, tunneled between the scapulae, and exteriorized via several small incisions. The pericardium was left open; the chest wall was closed in layers, and the pneumothorax was evacuated by a chest tube. Each dog was fitted with a jacket to prevent damage to the instruments and catheters that were housed in a jacket pocket. The pacing electrodes were attached to a programmable pacemaker that also was stored in a jacket pocket.
All dogs received fentanyl as needed after surgery. Dogs were allowed to recover a minimum of 7 days before experimentation. All dogs were treated with intramuscular antibiotics (cephalothin, 40 mg/kg, and gentamicin, 4.5 mg/kg) and trained to stand quietly in a sling during hemodynamic monitoring. Segment length signals were monitored by ultrasonic amplifiers. End-systolic (ESL) and end-diastolic segment length (EDL) were measured 30 ms before -dP/dtminand immediately before the onset of left ventricular isovolumic contraction, respectively. Percent segment shortening (%SS) was determined using the equation:%SS =(EDL - ESL)[center dot] 100 [center dot] EDL sup -1. The pressure work index (PWI), an estimate of global myocardial oxygen consumption, was calculated using a previously validated formula. [21 ] Hemodynamic data were continuously recorded on a polygraph and simultaneously digitized by a computer interfaced with an analog to digital converter for recording and subsequent analysis of left ventricular pressure-segment length waveforms and diagrams.
Experimental Protocol 
Each dog (n = 8; weight, 26.9 +/- 0.7 kg, mean +/- SEM) fasted overnight, and fluid deficits were replaced before experimentation with 0.9% saline (500 ml), which was continued at 3 ml [center dot] kg sup -1 [center dot] h sup -1 for the duration of each experiment. Left ventricular pressure-segment length diagrams used to assess myocardial contractility were obtained as previously described. [20 ] The slope (M sub w) of the regional preload recruitable stroke work relation derived from a series of differentially preloaded left ventricular pressure-segment length diagrams was used to quantify alterations in myocardial contractility. [22 ] A time constant of isovolumic relaxation (tau) was determined from the left ventricular pressure waveform at end-expiration using the derivative method. [23 ] After baseline systemic hemodynamics and left ventricular pressure-segment length diagrams had been recorded during steady-state conditions, escalating doses (0.2, 2.0, and 20.0 mg/kg, intravenous) of T3(3,3',5-triiodo-L-thyronine; Sigma Chemical, St. Louis, MO) were administered over 5 min at 1-h intervals. The drug vehicle for T3consisted of 25% ethanol (95%), 25% polyethylene glycol (5%), and 50% normal saline. No hemodynamic effects were produced by the drug vehicle alone. Peak changes in systemic hemodynamics were recorded, and left ventricular pressure-segment length diagrams were obtained using the techniques described above 10 min after the administration of each dose of T3. Hemodynamics and pressure-length diagrams were also obtained 24 h after T3administration. Plasma concentrations of T3were evaluated after each intervention using radioimmunoassay in five dogs.
Dogs were continuously paced at ventricular rates between 220 and 240 beats/min for 21 +/- 2 days to produce severe left ventricular dysfunction as indicated by chamber dilation, increases in left ventricular end-diastolic pressure, decreases in myocardial contractility, and increases in tau. [20 ] Dogs were brought to the laboratory on each day to confirm the functional integrity of the pacemaker and to monitor the development of pacing-induced cardiomyopathy. Pacing was temporarily discontinued during and restarted immediately after daily hemodynamic monitoring. After the development of pacing-induced cardiomyopathy, baseline hemodynamics, left ventricular pressure-segment length waveforms and diagrams, and plasma T3concentrations were recorded during steady-state conditions 30 min after pacing had been discontinued (sinus rhythm). Dogs then received T3in the doses described previously, and pacing was reinstated after data had been recorded. Hemodynamics, left ventricular pressure-length diagrams, and plasma T3concentrations were also obtained 24 h after the final dose of T3. Thus, the effects of T3on systemic hemodynamics, myocardial contractility, and isovolumic relaxation were studied in the same conscious, unsedated dogs before and after the development of rapid ventricular pacing-induced cardiomyopathy.
Statistical Analysis 
Statistical analysis of the data within and between groups before and after rapid ventricular pacing and during and after administration of T3was performed by multiple analysis of variance (ANOVA) with repeated measures, followed by Student's t test (two-tailed) with Duncan's adjustment for multiplicity. Changes were considered to be statistically significant when the P value was < 0.05. All data are expressed as mean +/- SEM.
Results 
Triiodothyronine caused significant (P < 0.05) increases in heart rate, rate-pressure product, pressure-work index, and cardiac output in conscious dogs with normal left ventricular function (Table 1). These variables remained elevated 24 h after T3administration. Mean arterial pressure, left ventricular systolic and end-diastolic pressures, EDL, ESL, systemic vascular resistance, and stroke volume were unchanged. Increases in Mw(88 +/- 5 during control to 109 +/- 4 mmHg after the 2 mg/kg dose) and dP/dtmax(2286 +/- 143 during control to 2593 +/- 193 mmHg/s after the 2 mg/kg dose) occurred with administration of T3, consistent with an immediate positive inotropic effect. T3-induced increases in myocardial contractility remained intact 24 h after administration of this hormone. No changes in tau occurred, suggesting that T3does not shorten the isovolumic relaxation phase of diastole in the normal heart.
Table 1. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Normal Left Ventricular Function 
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Table 1. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Normal Left Ventricular Function 
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Chronic rapid ventricular pacing produced marked changes in systemic hemodynamics and left ventricular function (e.g., Table 1vs. Table 2). Significant increases in baseline heart rate (sinus rhythm), left ventricular end-diastolic pressure, EDL, and ESL and decreases in mean arterial and left ventricular systolic pressures were observed after 21 +/- 2 days of pacing. No changes in calculated indices of myocardial oxygen consumption (rate-pressure product and pressure-work index), cardiac output, or systemic vascular resistance occurred with pacing. A decline in stroke volume was observed concomitant with an increase in heart rate. Rapid ventricular pacing caused a direct negative inotropic effect as indicated by reductions in Mw(88 +/- 5 before to 63 +/- 7 mmHg after pacing), dP/dtmax, and %SS. Pacing also increased tau (39 +/- 1 before to 65 +/- 8 ms after pacing), consistent with a negative lusitropic effect.
Table 2. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Left Ventricular Dysfunction 
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Table 2. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Left Ventricular Dysfunction 
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Triiodothyronine caused hemodynamic effects in dogs with pacing-induced cardiomyopathy that were somewhat different from those in dogs with normal left ventricular function (Table 2). Left ventricular end-diastolic pressure was significantly reduced (27 +/- 3 during control to 20 +/- 2 mmHg after the 2 mg/kg dose), and heart rate, rate-pressure product, and pressure-work index were unchanged during administration of T3to cardiomyopathic dogs in contrast to the findings in dogs before pacing. No changes in mean arterial and left ventricular systolic pressures, EDL, ESL, and systemic vascular resistance were observed. T3increased Mw(63 +/- 7 during control to 82 +/- 7 mmHg after the 2 mg/kg dose) and dP/dtmax(1585 +/- 120 during control to 1769 +/- 167 mmHg/s after the 20 mg/kg dose) in cardiomyopathic dogs, indicating a positive inotropic effect. The magnitude of increases in Mwand dP/dt sub max caused by T3was similar in dogs with and without pacing-induced cardiomyopathy (Figure 1(A and B)). An increase in stroke volume was observed 24 h after the administration of T3in cardiomyopathic dogs concomitant with increases in contractility despite significant reductions in left ventricular end-diastolic pressure. Decreases in tau (65 +/- 8 during control to 57 +/- 6 ms after the 20 mg/kg dose) occurred in dogs after chronic rapid ventricular pacing (Figure 1(C)). Similar to the findings in normal dogs, alterations in hemodynamics and left ventricular function produced by T3in cardiomyopathic dogs persisted 24 h after administration of this hormone.
Figure 1. Histograms illustrating the percent change from control of regional preload recruitable stroke work slope (Mw, A), left ventricular dP/dtmax(B), and the time constant of isovolumic relaxation (tau; C) produced immediately by escalating doses of triiodothyronine (T3; 0.2, 2.0, and 20.0 mg/kg, intravenous) and 24 h after T3administration in dogs before (normal; solid bars) and after the development of rapid ventricular pacing-induced left ventricular dysfunction (cardiomyopathy; hatched bars). *Significantly (P < 0.05) different from normal.
Figure 1. Histograms illustrating the percent change from control of regional preload recruitable stroke work slope (Mw, A), left ventricular dP/dtmax(B), and the time constant of isovolumic relaxation (tau; C) produced immediately by escalating doses of triiodothyronine (T3; 0.2, 2.0, and 20.0 mg/kg, intravenous) and 24 h after T3administration in dogs before (normal; solid bars) and after the development of rapid ventricular pacing-induced left ventricular dysfunction (cardiomyopathy; hatched bars). *Significantly (P < 0.05) different from normal.
Figure 1. Histograms illustrating the percent change from control of regional preload recruitable stroke work slope (Mw, A), left ventricular dP/dtmax(B), and the time constant of isovolumic relaxation (tau; C) produced immediately by escalating doses of triiodothyronine (T3; 0.2, 2.0, and 20.0 mg/kg, intravenous) and 24 h after T3administration in dogs before (normal; solid bars) and after the development of rapid ventricular pacing-induced left ventricular dysfunction (cardiomyopathy; hatched bars). *Significantly (P < 0.05) different from normal.
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Discussion 
The present results in conscious dogs with normal left ventricular function indicate that T3causes acute increases in myocardial contractility, confirming the findings of several previous investigations in normal papillary muscle [24,25 ] and isolated heart preparations. [26–28 ] The present results also show that T3produces immediate and sustained declines in left ventricular end-diastolic pressure, increases of myocardial contractility, improvements in isovolumic relaxation, and modest increases in cardiac output in dogs with pacing-induced cardiomyopathy. Importantly, these beneficial hemodynamic effects occurred in the absence of increases in heart rate and calculated estimates of global myocardial oxygen consumption in dogs with severe left ventricular dysfunction. These findings contrast with those observed in dogs before pacing, in which the positive inotropic actions of T3occurred concomitant with tachycardia and increases in rate-pressure product and pressure-work index. The results in cardiomyopathic dogs suggest that myocardial oxygen consumption remained constant during and after administration of T3because increases in myocardial contractility were balanced by simultaneous reductions in left ventricular end-diastolic pressure. This conclusion requires qualification because coronary sinus oxygen tension was not measured and because myocardial oxygen consumption was not directly determined in vivo. The baseline plasma T3concentrations observed in conscious dogs before and after the development of pacing-induced cardiomyopathy (0.49 +/- 0.06 and 0.40 +/- 0.10 ng/ml, respectively) were similar to those reported previously in an identical model. [18 ] However, these T3levels are below the normal range (0.71 to 1.82 ng/ml) for healthy dogs in the absence of chronic surgical instrumentation. Thus, the present results should also be qualified because the effects of T3on systemic hemodynamics and left ventricular function were examined in normal and cardiomyopathic dogs with laboratory evidence of hypothyroidism.
The modest positive inotropic and lusitropic effects of T3observed in the present investigation are supported by the findings of several previous studies in experimental models of acute or chronic left ventricular dysfunction. T3has been shown to enhance contractile function without producing adverse increases in myocardial oxygen consumption or decreases in mechanical efficiency after global hypothermic ischemia in isolated canine hearts. [29 ] T3and related structural analogs of thyroid hormone also have been shown to produce positive inotropic effects in stunned myocardium. [2–4 ] The immediate and sustained actions of T3in cardiomyopathic dogs in the present investigation also are firmly supported by the previous observations of Morkin et al. [5 ] and Mahaffey et al. [4 ] in rats and rabbits, respectively, showing that T3causes increases in dP/dtmaxand beneficial reductions in left ventricular end-diastolic pressure and tau in heart failure produced by myocardial infarction and ventricular remodeling.
Thyroid hormone therapy produces beneficial effects in patients after acute myocardial infarction [7,8 ] and sudden cardiac death. [9 ] Clinical evidence has suggested that T3supplementation may acutely enhance cardiac performance, hasten the recovery of normal hemodynamics, and reduce the need for inotropic support in patients with left ventricular dysfunction after cardiopulmonary bypass, [10,11,30,31 ] although these conclusions have been challenged by recently published data. [32–34 ] Oral administration of T4for 1 week has also been shown to increase ejection fraction, cardiac output, and exercise tolerance in patients with dilated cardiomyopathy. [12 ] Thus, the present results with T3in conscious, cardiomyopathic dogs are also qualitatively supported by the majority of previous findings in patients with acute left ventricular dysfunction or chronic congestive heart failure.
Triiodothyronine has been shown to acutely enhance the activity of Ca2+-ATPases in the sarcolemmal membrane and the sarcoplasmic reticulum (SR). [35 ] These actions lead to increased SR Ca2+ uptake and more efficient Ca2+ extrusion from the cardiac myocyte during diastole, effects that may have contributed to observed decreases in tau in cardiomyopathic dogs in the present investigation. Reversible declines in the rate of left ventricular isovolumic relaxation have also been observed previously in patients with acute hypothyroidism concomitant with reductions in myocardial contractility. [36 ] Thus, enhanced sarcolemmal and SR Ca2+-ATPase activity during the administration of T3may partially attenuate abnormalities of intracellular Ca2+ homeostasis that are central to the pathogenesis of diastolic dysfunction in rapid pacing-induced [15 ] and clinical heart failure. [37 ] Potentiation of SR Ca2+-ATPase activity by T3probably contributes to immediate increases in myocardial contractility by increasing the quantity of Ca2+ stored in this organelle for subsequent release during the next systole. T3has also been shown to directly enhance Ca2+ influx across the sarcolemmal membrane [38–40 ] via the voltage-dependent Ca2+ channel. [38,41 ] This increase in Ca2+ influx associated with membrane depolarization may contribute to the acute positive inotropic actions of T3not only by directly increasing Ca2+ availability for contractile activation but also by simultaneously stimulating Ca2+-induced Ca2+ release from the SR.
The persistent increases in Mwand dP/dtmaxand decreases in tau observed in dogs with pacing-induced left ventricular dysfunction 24 h after administration of T3are consistent with the known time-course of nuclear thyroid hormone receptor upregulation [42,43 ] and the subsequent transcription and translation of SR Ca2+-ATPase. [44 ] T3may favorably alter the composition of myosin heavy chain isoforms in the failing heart, [45 ] although recent evidence suggests that this effect plays a relatively minor role in chronic T3-induced increases in contractility in heart failure caused by myocardial infarction and ventricular remodeling. [6 ] beta-Adrenoceptor downregulation, reduced adenylyl cyclase activity, and decreased production of cyclic adenosine monophosphate (cAMP) are characteristic features that contribute to depressed contractility in failing myocardium. Although the beta-adrenoceptor-linked signal transduction is not responsible for the inotropic actions of T3in normal myocardium, [27 ] T3may enhance beta-adrenoceptor density [46 ] and sensitivity [47 ] in hypothyroid states, including those associated with heart failure. Such an improvement in beta-adrenoceptor viability by T3may contribute to the transient and sustained positive inotropic effects of this hormone in failing myocardium. This hypothesis remains to be tested; the stability of heart rate observed during and after the administration of T3to cardiomyopathic dogs suggests that the beneficial actions of this thyroid hormone on left ventricular systolic and diastolic function were not based solely on alterations in beta-adrenoceptor activity.
In summary, the present results indicate that T3produces immediate and sustained increases in myocardial contractility and improvement in isovolumic relaxation in conscious dogs with rapid ventricular pacing-induced cardiomyopathy. These positive inotropic and lusitropic effects were not accompanied by increases in myocardial oxygen consumption because T3caused favorable reductions in left ventricular end-diastolic pressure without changes in heart rate.
The authors thank Ms. Angela M. Barnes for preparation of the manuscript.
References 
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Figure 1. Histograms illustrating the percent change from control of regional preload recruitable stroke work slope (Mw, A), left ventricular dP/dtmax(B), and the time constant of isovolumic relaxation (tau; C) produced immediately by escalating doses of triiodothyronine (T3; 0.2, 2.0, and 20.0 mg/kg, intravenous) and 24 h after T3administration in dogs before (normal; solid bars) and after the development of rapid ventricular pacing-induced left ventricular dysfunction (cardiomyopathy; hatched bars). *Significantly (P < 0.05) different from normal.
Figure 1. Histograms illustrating the percent change from control of regional preload recruitable stroke work slope (Mw, A), left ventricular dP/dtmax(B), and the time constant of isovolumic relaxation (tau; C) produced immediately by escalating doses of triiodothyronine (T3; 0.2, 2.0, and 20.0 mg/kg, intravenous) and 24 h after T3administration in dogs before (normal; solid bars) and after the development of rapid ventricular pacing-induced left ventricular dysfunction (cardiomyopathy; hatched bars). *Significantly (P < 0.05) different from normal.
Figure 1. Histograms illustrating the percent change from control of regional preload recruitable stroke work slope (Mw, A), left ventricular dP/dtmax(B), and the time constant of isovolumic relaxation (tau; C) produced immediately by escalating doses of triiodothyronine (T3; 0.2, 2.0, and 20.0 mg/kg, intravenous) and 24 h after T3administration in dogs before (normal; solid bars) and after the development of rapid ventricular pacing-induced left ventricular dysfunction (cardiomyopathy; hatched bars). *Significantly (P < 0.05) different from normal.
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Table 1. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Normal Left Ventricular Function 
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Table 1. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Normal Left Ventricular Function 
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Table 2. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Left Ventricular Dysfunction 
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Table 2. Hemodynamic Effects of Triiodothyronine in Conscious Dogs with Left Ventricular Dysfunction 
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