Free
Meeting Abstracts  |   September 2005
Involvement of Ca2+Sensitization in Ropivacaine-induced Contraction of Rat Aortic Smooth Muscle
Author Affiliations & Notes
  • Jingui Yu, M.D.
    *
  • Yasuyuki Tokinaga, M.D.
  • Toshiyuki Kuriyama, M.D.
  • Nobuhiko Uematsu, M.D.
    §
  • Kazuhiro Mizumoto, M.D.
  • Yoshio Hatano, M.D.
    #
  • * Research Fellow, † Graduate Student, § Staff Anesthesiologist, ∥ Assistant Professor, # Professor and Chairman, Department of Anesthesiology, ‡ Staff Anesthesiologist, Department of Surgical Operating Center, Wakayama Medical University.
Article Information
Meeting Abstracts   |   September 2005
Involvement of Ca2+Sensitization in Ropivacaine-induced Contraction of Rat Aortic Smooth Muscle
Anesthesiology 9 2005, Vol.103, 548-555. doi:
Anesthesiology 9 2005, Vol.103, 548-555. doi:
MANY in vitro  and in vivo  studies have elucidated that amino-amide local anesthetic agents such as lidocaine, bupivacaine, mepivacaine and ropivacaine exhibit biphasic vascular effects, to varying extents: vasoconstriction at low concentrations and decrease of contraction or even vasodilation at high concentrations.1–8 The primary results in our current in vitro  study demonstrated that these local anesthetics also exhibit biphasic vascular effects in rat aortic smooth muscle, with a strongest contractile response to ropivacaine (fig. 1). However, the molecular mechanism of the amino-amide local anesthetic agent–induced vasoconstriction remains unclear. Because ropivacaine is a new, long-acting, amino-amide local anesthetic and evokes potent vasoconstriction, regardless of the route of administration,9–11 we chose ropivacaine as representative of amino-amide local anesthetics to study their mechanism of vasoconstriction.
Fig. 1. Amino-amide local anesthetic agent–induced, dose-dependent, biphasic contraction of rat aortic smooth muscle. Ropivacaine, bupivacaine, mepivacaine, and lidocaine were delivered to the endothelium-denuded rings in a cumulative dose manner. The resultant tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride (KCl)–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  lidocaine. n = 6 (  A  ). A recording of the ropivacaine-induced contraction was presented (  B  ). 
Fig. 1. Amino-amide local anesthetic agent–induced, dose-dependent, biphasic contraction of rat aortic smooth muscle. Ropivacaine, bupivacaine, mepivacaine, and lidocaine were delivered to the endothelium-denuded rings in a cumulative dose manner. The resultant tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride (KCl)–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  lidocaine. n = 6 (  A  ). A recording of the ropivacaine-induced contraction was presented (  B  ). 
Fig. 1. Amino-amide local anesthetic agent–induced, dose-dependent, biphasic contraction of rat aortic smooth muscle. Ropivacaine, bupivacaine, mepivacaine, and lidocaine were delivered to the endothelium-denuded rings in a cumulative dose manner. The resultant tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride (KCl)–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  lidocaine. n = 6 (  A  ). A recording of the ropivacaine-induced contraction was presented (  B  ). 
×
Smooth muscle contraction involves complex mechanisms, mainly calcium (Ca2+)–dependent12 and Ca2+-independent (or Ca2+-sensitization) mechanisms. It is well known that potassium chloride (KCl)–induced smooth muscle contraction is mediated solely by Ca2+-dependent mechanisms, i.e.  , via  extracellular Ca2+influx through L-type Ca2+channels, without involvement of Ca2+-sensitization mechanisms. The primary results of our study demonstrated that the ropivacaine-induced force/intracellular Ca2+concentration ([Ca2+]i) ratio in rat aortic smooth muscle is higher than that in response to KCl (figs. 1B and 2A), suggesting that, in addition to the Ca2+-dependent mechanism, Ca2+sensitization is likely also involved in ropivacaine-induced vascular contraction.
Fig. 2. Ropivacaine (Rop)–induced, dose-dependent, biphasic increase in intracellular calcium concentration ([Ca2+]i) of rat aortic smooth muscle. The [Ca2+]iof fura-2–loaded aortic strips was detected using fluorometer and was expressed as the ratio F340/F380 (  A  ). The [Ca2+]ilevels stimulated by ropivacaine are presented as a percent of the 30 mm potassium chloride (KCl)–induced value (  B  ). *  P  < 0.05, **  P  < 0.01  versus  baseline (n = 6). 
Fig. 2. Ropivacaine (Rop)–induced, dose-dependent, biphasic increase in intracellular calcium concentration ([Ca2+]i) of rat aortic smooth muscle. The [Ca2+]iof fura-2–loaded aortic strips was detected using fluorometer and was expressed as the ratio F340/F380 (  A  ). The [Ca2+]ilevels stimulated by ropivacaine are presented as a percent of the 30 mm potassium chloride (KCl)–induced value (  B  ). *  P  < 0.05, **  P  < 0.01  versus  baseline (n = 6). 
Fig. 2. Ropivacaine (Rop)–induced, dose-dependent, biphasic increase in intracellular calcium concentration ([Ca2+]i) of rat aortic smooth muscle. The [Ca2+]iof fura-2–loaded aortic strips was detected using fluorometer and was expressed as the ratio F340/F380 (  A  ). The [Ca2+]ilevels stimulated by ropivacaine are presented as a percent of the 30 mm potassium chloride (KCl)–induced value (  B  ). *  P  < 0.05, **  P  < 0.01  versus  baseline (n = 6). 
×
Ca2+-sensitization mechanisms refer primarily to protein kinase C (PKC)–,13 Rho kinase–,14 and p44/42 mitogen-activated protein kinase (p44/42 MAPK)–mediated15 signaling pathways, which play an important role in maintaining and regulating vascular tension. The current study is designed to investigate the role of Ca2+-sensitization mechanisms, including PKC, Rho kinase, and p44/42 MAPK, in mediating ropivacaine-induced vasoconstriction by measurement of ropivacaine-induced contraction and the activation of PKC, Rho kinase, and p44/42 MAPK in rat aortic smooth muscle.
Materials and Methods
Measurement of Isometric Tension
The protocol was approved by the Animal Care and Use Committee of Wakayama Medical University (Wakayama City, Japan). Tissue preparation and tension measurement were performed as described previously.16–18 In brief, male Wistar rats (300–400 g) were anesthetized with halothane and were exsanguinated by bleeding from the common carotid artery. The descending thoracic aorta was harvested, and eight endothelium-denuded rings (3–4 mm in length) were prepared from each rat. The aortic rings were mounted to isometric force transducers (Nihondenki-sanei Co., Tokyo, Japan) and incubated in Krebs bicarbonate solution (consisting of 118.2 mm sodium chloride, 4.6 mm KCl, 2.5 mm calcium chloride, 1.2 mm monobasic potassium phosphate, 1.2 mm magnesium sulfate heptahydrate, 24.8 mm sodium hydrogen carbonate, and 10.0 mm dextrose) maintained at 37°C and gassed continuously with a mixture of 95% oxygen–5% carbon dioxide (pH 7.4). The solution contained the cyclooxygenase inhibitor indomethacin (10−5m) and the nitric oxide synthase inhibitor N  -nitro-l-arginine methyl ester (10−4m) to prevent the release of endogenous prostaglandin I2and nitric oxide, respectively, from any residual endothelium. The rings were equilibrated for 1 h at a resting tension of 3 g, overall contractile responsiveness was assessed with KCl (3 × 10−2m), and complete removal of the endothelium was confirmed with acetylcholine (10−5m). The tension experiment was performed with several subtype protocols. The local anesthetic agent–induced contractile responses were expressed as a percentage of the KCl (3 × 10−2m)–induced contraction.
The cumulative dose–response relations of the rings to ropivacaine, bupivacaine, mepivacaine, and lidocaine (3 × 10−5m to 10−2m) were examined to compare their vascular effects. Subsequent doses of agents were administered after the previous dose elicited a sustained and stable contraction for 3–5 min. One ring from each animal randomly underwent the cumulative dose administration of one local anesthetic agent once.
The cumulative dose (3 × 10−5m to 10−2m)–contractile response to ropivacaine was tested in the presence of the PKC inhibitors bisindolylmaleimide I (competing with adenosine triphosphate for binding to the catalytic domain, 10−6m, 5 × 10−6m, and 10−5m) and calphostin C (competing with phorbol 12-myristate 13-acetate or diglyceride for binding to the regulatory domain, 10−7m, 5 × 10−7m, and 10−6m), the Rho-kinase inhibitor Y 27632 (5 × 10−7m, 10−6m, and 5 × 10−6m), or the p44/42 MAPK inhibitor PD 098059 (10−5m, 5 × 10−5m, and 10−4m). The effects of the combination of these inhibitors (bisindolylmaleimide I [10−6m] plus PD 098059 [5 × 10−7m]; bisindolylmaleimide I [10−6m] plus Y 27632 [10−5m]; Y 27632 [5 × 10−7m] plus PD 098059 [10−5m]; and all three inhibitors) on the ropivacaine-induced cumulative dose–contractile response were also measured. All of these inhibitors were delivered to the rings 15 min before the application of ropivacaine and maintained until the end of measurement (preadministration). One ring from each animal was randomly challenged by only one concentration of an inhibitor or one combination of inhibitors, followed by the cumulative dose administration of ropivacaine,
In another group of rings, bisindolylmaleimide I (5 × 10−6), calphostin C (5 × 10−7m), Y 27632 (10−6m), and PD 098059 (5 × 10−5m) were administered after a sustained ropivacaine (3 × 10−4m)–induced contraction had been achieved to observe their inhibitory effects on ropivacaine-elicited contraction (postadministration). One ring from each animal was randomly treated with only one inhibitor after receiving a single dose of ropivacaine.
Measurement of Intracellular Ca2+Concentration
Endothelium-denuded aortic segments were treated with a 10−5-m acetoxymethyl ester of fura-2 solution for 6–9 h at room temperature and were then fixed to a fluorometer (CAF-110; Japan Spectroscopic, Tokyo, Japan). The intimal surface of the muscle strip was illuminated at 50 Hz at alternating excitation wavelengths of 340 and 380 nm, and the amount of fluorescence at 510 nm induced by 340 nm excitation (F340) and that induced by 380 nm excitation (F380) were measured. The ratio F340/F380 was used to indicate the [Ca2+]i.
The ratio F340/F380 induced by 3 × 10−2m KCl was measured first, and the values were considered as the reference (100%). Thereafter, the cumulative dose (3 × 10−5m to 3 × 10−3m)–response of the ratio F340/F380 to ropivacaine was tested and was expressed as a percentage of the reference values.
Detection of Protein Kinase Activation
The phosphorylation of PKC and p44/42 MAPK, and membrane translocation of Rho kinase (Rock-2) were detected using Western blotting with specific antibodies, as described previously.13–15 Briefly, the endothelium-denuded rat aortic strips (approximately 3.5 cm in length) were treated randomly with ropivacaine at concentrations of 3 × 10−5m, 10−4m, 3 × 10−4m, 10−3m, or 3 × 10−3m for 20 min, respectively, to measure the dose–response relation of ropivacaine-induced phosphorylation of PKC and p44/42 MAPK and Rho-kinase membrane translocation. Some strips were pretreated with bisindolylmaleimide I (10−5m), calphostin C (10−6m), Y 27632 (5 × 10−6m), or PD 098059 (10−4m) for 15 min before challenge by ropivacaine (3 × 10−4m). Each animal provided only one aortic strip, and each strip was treated randomly with only one concentration of ropivacaine in the presence or absence of each inhibitor.
The agent-treated aortic strips were quickly frozen with dry ice and homogenized in lysis buffer (50 mm HEPES, pΩ 7.5, 1% Triton X-100, 50 mm sodium chloride, 50 mm sodium fluoride, 5 mm EDTA, 10 mm sodium pyrophosphate, 1 mm phenylmethanesulfonyl fluoride, 1 mm sodium orthovanadate, 10 μg/ml leupeptin, and 20 μg/ml aprotinin).19 Homogenates were centrifuged at 15,000g  for 15 min at 4°C. The supernatant was collected for the detection of PKC and p44/42MAPK phosphorylation. For the measurement of Rho-kinase membrane translocation, the homogenates were centrifuged at 13,000g  for 3 min at 4°C, and the supernatant was collected and then centrifuged at 100,000g  for 30 min at 4°C. The supernatant (cytosolic fraction) was removed, and the pellet (membrane fraction) was resuspended using the same buffer. The protein concentration of each sample was determined using the bicinchoninic acid method.20 
Equal amounts of total protein (20–30 μg) were used for every sample in each experiment. Proteins were separated by sodium-dodecyl-sulfate polyacrylamide gel electrophoresis and were transferred to nitrocellulose membrane. The membrane was treated with anti-PKC (1:1,000), anti–phospho-PKC (pan, βIISer660, 1:1,000), anti–p44/42 MAPK (1:2,000), anti–phospho-p44/42 MAPK (Thr/Tyr204, 1:2,000), or anti–Rock-2 (1:1,000) antibodies, as appropriate, for 2 h, followed by incubation with horseradish peroxidase–conjugated antibody (1:2,000) for 1 h at room temperature. Immunoreactive bands were detected using chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ) and were assessed with ATTO Lane Analyzer 10H1.02 (ATTO Densitograph Software Library, Tokyo, Japan). The values for phosphorylation of PKC and p44/42 MAPK were expressed as a percentage of the density of total PKC and total p44/42 MAPK bands, respectively. The amount of Rock-2 in the membrane fraction was expressed as a percent of total Rock-2 value (i.e.  , membrane fraction plus cytosolic fraction). The sample size (n) value represents the number of aortic strips from the same number of rats for each concentration of ropivacaine.
Materials
Ropivacaine was a kind gift from AstraZeneca (Osaka, Japan). Lidocaine, bupivacaine, mepivacaine, bisindolylmaleimide I, calphostin C, and PD 098059 were purchased from Sigma-Aldrich Fine Chemicals (St. Louis, MO). Y 27632 was provided by Calbiochem-Novabiochem Corporation (San Diego, CA). Polyclonal antibodies against phospho-PKC (pan, βIISer660), p44/42 MAPK, and phospho-p44/42MAPK (Thr/Tyr204) were supplied by Cell Signaling Technology Inc. (Beverly, MA). Polyclonal antibodies against PKC (H-300) and Rock-2 and the secondary antibody labeled with horseradish peroxidase were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). All other reagents for the experiments were of analytical grade.
Data Analysis
All data are presented as mean ± SD. The sample size (n value) represents the number of rats from which aortic rings were taken for each protocol. Differences among anesthetic agents at the same concentration and the effects of different concentrations of inhibitors on ropivacaine at same concentration were tested by one-way analysis of variance followed by an unpaired Student t  test with a Bonferroni correction. The ropivacaine-induced dose-dependent changes in [Ca2+]iand protein kinase activation were analyzed by two-way analysis of variance for repeated measures and a paired Student t  test with a Bonferroni correction for post hoc  comparisons. P  values less than 0.05 were considered statistically significant.
Results
Amino-amide Local Anesthetic–induced Contractile Responses of Rat Aortic Smooth Muscle
All of the amino-amide local anesthetics tested induced dose-dependent, biphasic contraction of rat aortic endothelium-denuded rings. The rank order for degree of vasoconstriction was ropivacaine > bupivacaine > mepivacaine > lidocaine. The concentrations required to achieve the maximal contraction are 10−4m, 3 × 10−4m, 3 × 10−3m, and 3 × 10−3m for bupivacaine, ropivacaine, mepivacaine, and lidocaine, respectively (fig. 1). Because ropivacaine has strongest contractile properties among the amino-amide local anesthetics, we focused on the elucidation of its contractile mechanism in the following experiments.
Ropivacaine-elicited Increase in Intracellular Ca2+Concentration
Ropivacaine elicited a dose-dependent biphasic change in [Ca2+]i(F340/F380), which was consistent with the tension pattern: increase from concentration of 3 × 10−5m to 3 × 10−4m, decrease from concentration of 10−3m to 3 × 10−3m (figs. 2A and B). The ratio of ropivacaine-induced force/[Ca2+]iwas higher than that induced by KCl.
Effects of PKC, Rho-Kinase, and p44/42 MAPK Inhibitors on Ropivacaine-induced Contraction of Rat Aortic Smooth Muscle
The PKC inhibitors bisindolylmaleimide I and calphostin C, the Rho-kinase inhibitor Y 27632, and the p44/42 MAPK inhibitor PD 098059 almost did not change the baseline resting tension, even at their highest concentrations used (n = 6; figs. 3A and B). However, these inhibitors significantly attenuated the sustained ropivacaine (3 × 10−4m)–induced contraction (n = 6; figs. 3C and D).
Fig. 3. Effects of the protein kinase inhibitors on the resting tension (  A  and  B  ) and ropivacaine (Rop)–induced sustained contraction (  C  and  D  ) of rat aortic smooth muscle. The tension was measured using isometric force transducers. Endothelium-denuded rings were equilibrated for 1 h at a resting tension of 3 g and then exposed to different concentrations of bisindolylmaleimide I (Bis I) for 15 min (  A  ). The statistical data for the effects of all of the inhibitors used on resting tension are presented in  B  . The resting tension (3 g) was considered as 100% (n = 6). A bolus of ropivacaine (3 × 10−4m) was applied to the rings to induce a sustained contraction, and Bis I was then delivered (  C  ). The statistical data for the effects of all of the inhibitors used on sustained ropivacaine-induced contraction are presented in  D  . Ropivacaine-induced contraction was expressed as a percent of the 30 mm potassium chloride–induced contraction. **  P  < 0.01  versus  control (n = 6). Calp C = calphostin C; PD = PD 098059; Y = Y 27632. 
Fig. 3. Effects of the protein kinase inhibitors on the resting tension (  A  and  B  ) and ropivacaine (Rop)–induced sustained contraction (  C  and  D  ) of rat aortic smooth muscle. The tension was measured using isometric force transducers. Endothelium-denuded rings were equilibrated for 1 h at a resting tension of 3 g and then exposed to different concentrations of bisindolylmaleimide I (Bis I) for 15 min (  A  ). The statistical data for the effects of all of the inhibitors used on resting tension are presented in  B  . The resting tension (3 g) was considered as 100% (n = 6). A bolus of ropivacaine (3 × 10−4m) was applied to the rings to induce a sustained contraction, and Bis I was then delivered (  C  ). The statistical data for the effects of all of the inhibitors used on sustained ropivacaine-induced contraction are presented in  D  . Ropivacaine-induced contraction was expressed as a percent of the 30 mm potassium chloride–induced contraction. **  P  < 0.01  versus  control (n = 6). Calp C = calphostin C; PD = PD 098059; Y = Y 27632. 
Fig. 3. Effects of the protein kinase inhibitors on the resting tension (  A  and  B  ) and ropivacaine (Rop)–induced sustained contraction (  C  and  D  ) of rat aortic smooth muscle. The tension was measured using isometric force transducers. Endothelium-denuded rings were equilibrated for 1 h at a resting tension of 3 g and then exposed to different concentrations of bisindolylmaleimide I (Bis I) for 15 min (  A  ). The statistical data for the effects of all of the inhibitors used on resting tension are presented in  B  . The resting tension (3 g) was considered as 100% (n = 6). A bolus of ropivacaine (3 × 10−4m) was applied to the rings to induce a sustained contraction, and Bis I was then delivered (  C  ). The statistical data for the effects of all of the inhibitors used on sustained ropivacaine-induced contraction are presented in  D  . Ropivacaine-induced contraction was expressed as a percent of the 30 mm potassium chloride–induced contraction. **  P  < 0.01  versus  control (n = 6). Calp C = calphostin C; PD = PD 098059; Y = Y 27632. 
×
The pretreatment with bisindolylmaleimide I, calphostin C, Y27632, and PD 098059 dose-dependently inhibited the dose-dependent ropivacaine-induced contractile response of endothelium-denuded rat aortic rings (figs. 4A–D). The combinations of bisindolylmaleimide I (10−6m) plus PD 098059 (5 × 10−7m), bisindolylmaleimide I (10−6m) plus Y 27632 (10−5m), and Y 27632 (5 × 10−7m) plus PD 098059 (10−5m) resulted in further attenuation of the ropivacaine-induced contraction compared to any single inhibitor at the same concentration, with much greater inhibition with the latter two combinations than with the first one. The combination of all three inhibitors almost abolished the ropivacaine-induced contraction (fig. 5).
Fig. 4. Effects of the protein kinase C inhibitor bisindolylmaleimide I (Bis I;  A  ), calphostin C (Calp C;  B  ), the Rho-kinase inhibitor Y 27632 (Y;  C  ), and the p44/42 MAPK inhibitor PD 098059 (PD;  D  ) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with the above-mentioned inhibitors at different concentrations for 15 min and were then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 4. Effects of the protein kinase C inhibitor bisindolylmaleimide I (Bis I;  A  ), calphostin C (Calp C;  B  ), the Rho-kinase inhibitor Y 27632 (Y;  C  ), and the p44/42 MAPK inhibitor PD 098059 (PD;  D  ) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with the above-mentioned inhibitors at different concentrations for 15 min and were then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 4. Effects of the protein kinase C inhibitor bisindolylmaleimide I (Bis I;  A  ), calphostin C (Calp C;  B  ), the Rho-kinase inhibitor Y 27632 (Y;  C  ), and the p44/42 MAPK inhibitor PD 098059 (PD;  D  ) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with the above-mentioned inhibitors at different concentrations for 15 min and were then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
×
Fig. 5. Effects of combining bisindolylmaleimide I (Bis I; 10−6m), Y 27632 (Y; 5 × 10−7m), and PD 098059 (PD; 10−5m) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with one of the four combinations for 15 min and then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 5. Effects of combining bisindolylmaleimide I (Bis I; 10−6m), Y 27632 (Y; 5 × 10−7m), and PD 098059 (PD; 10−5m) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with one of the four combinations for 15 min and then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 5. Effects of combining bisindolylmaleimide I (Bis I; 10−6m), Y 27632 (Y; 5 × 10−7m), and PD 098059 (PD; 10−5m) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with one of the four combinations for 15 min and then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
×
Ropivacaine-induced Phosphorylation of PKC and p44/42 MAPK and Membrane Translocation of Rho Kinase
Anti-PKC and anti–phospho-PKC antibodies can recognize several PKC subtypes, including PKC-α, -β1, -β2, -ε, -η, and -δ, with molecular weights between 78 and 85 kd, which appeared as double bands in the current study (figs. 6A and B). Bands with molecular weights of 150, 44, and 42 kd were confirmed as Rock-2 (fig. 6C), p44, and p42 MAPK (fig. 6D), respectively. No differences were detected in the density of the PKC or p44/42 MAPK or the total density of Rock-2 (i.e.  , membrane fraction plus cytosolic fraction) bands at each dose of ropivacaine. However, the densities of the phosphorylated PKC and phosphorylated p44/42 MAPK bands and the density of the Rock-2 band in the membrane fraction changed in accord with the ropivacaine treatment: increasing at 3 × 10−5m and 10−4m, reaching the maximum level at 3 × 10−4m, and decreasing from 10−3m to 3 × 10−3m (figs. 6A–D), which was consistent with the contractile response. The presence of bisindolylmaleimide I (10−5m), calphostin C (10−6m), Y 27632 (5 × 10−6m), or PD 098059 (10−4m) almost completely abrogated the ropivacaine (3 × 10−4m)–induced increase in the densities of the phosphorylated PKC and p44/42 MAPK bands and the density of the Rock-2 band in the membrane fraction, respectively (figs. 6A–D).
Fig. 6. Ropivacaine (Rop)–induced phosphorylation of protein kinase C (PKC) and p44/42 mitogen-activated protein kinase (p44/42 MAPK) and Rho-kinase membrane translocation in rat aortic smooth muscle. Endothelium-denuded strips were randomly challenged by different concentrations of ropivacaine for 20 min or were first pretreated with bisindolylmaleimide I (Bis I; 10−5m) (  A  ), calphostin C (Calp C; 10−6m) (  B  ), Y 27632 (Y; 5 × 10−6m) (  C  ), or PD 098059 (PD; 10−4m) (  D  ) for 15 min and then challenged with ropivacaine (3 × 10−4m) for 20 min. PKC and p44/42 MAPK phosphorylation and Rho-kinase (Rock-2) membrane translocation were detected using Western blotting with specific antibodies. Values for phosphorylation of PKC and p44/42 MAPK were expressed as percentages of the total PKC and total p44/42 MAPK, respectively. The level of Rock-2 in the membrane fraction was expressed as a percentage of the total Rock-2 (  i.e.  , membrane fraction plus cytosolic fraction). *  P  < 0.05,  P  < 0.01  versus  control (0 m) (n = 4). C = cytosolic fraction; M = membrane fraction; p-p44/42 MAPK = phosphorylated p44/42 mitogen-activated protein kinase; p-PKC = phosphorylated protein kinase C. 
Fig. 6. Ropivacaine (Rop)–induced phosphorylation of protein kinase C (PKC) and p44/42 mitogen-activated protein kinase (p44/42 MAPK) and Rho-kinase membrane translocation in rat aortic smooth muscle. Endothelium-denuded strips were randomly challenged by different concentrations of ropivacaine for 20 min or were first pretreated with bisindolylmaleimide I (Bis I; 10−5m) (  A  ), calphostin C (Calp C; 10−6m) (  B  ), Y 27632 (Y; 5 × 10−6m) (  C  ), or PD 098059 (PD; 10−4m) (  D  ) for 15 min and then challenged with ropivacaine (3 × 10−4m) for 20 min. PKC and p44/42 MAPK phosphorylation and Rho-kinase (Rock-2) membrane translocation were detected using Western blotting with specific antibodies. Values for phosphorylation of PKC and p44/42 MAPK were expressed as percentages of the total PKC and total p44/42 MAPK, respectively. The level of Rock-2 in the membrane fraction was expressed as a percentage of the total Rock-2 (  i.e.  , membrane fraction plus cytosolic fraction). *  P  < 0.05,  P  < 0.01  versus  control (0 m) (n = 4). C = cytosolic fraction; M = membrane fraction; p-p44/42 MAPK = phosphorylated p44/42 mitogen-activated protein kinase; p-PKC = phosphorylated protein kinase C. 
Fig. 6. Ropivacaine (Rop)–induced phosphorylation of protein kinase C (PKC) and p44/42 mitogen-activated protein kinase (p44/42 MAPK) and Rho-kinase membrane translocation in rat aortic smooth muscle. Endothelium-denuded strips were randomly challenged by different concentrations of ropivacaine for 20 min or were first pretreated with bisindolylmaleimide I (Bis I; 10−5m) (  A  ), calphostin C (Calp C; 10−6m) (  B  ), Y 27632 (Y; 5 × 10−6m) (  C  ), or PD 098059 (PD; 10−4m) (  D  ) for 15 min and then challenged with ropivacaine (3 × 10−4m) for 20 min. PKC and p44/42 MAPK phosphorylation and Rho-kinase (Rock-2) membrane translocation were detected using Western blotting with specific antibodies. Values for phosphorylation of PKC and p44/42 MAPK were expressed as percentages of the total PKC and total p44/42 MAPK, respectively. The level of Rock-2 in the membrane fraction was expressed as a percentage of the total Rock-2 (  i.e.  , membrane fraction plus cytosolic fraction). *  P  < 0.05,  P  < 0.01  versus  control (0 m) (n = 4). C = cytosolic fraction; M = membrane fraction; p-p44/42 MAPK = phosphorylated p44/42 mitogen-activated protein kinase; p-PKC = phosphorylated protein kinase C. 
×
Discussion
The main findings of the current study are as follows: (1) Amino-amide local anesthetics induced a dose-dependent, biphasic response of rat aortic endothelium-denuded rings. Ropivacaine induced the strongest contraction among them, which was attenuated in a dose-dependent manner by the PKC inhibitors bisindolylmaleimide I and calphostin C, the Rho-kinase inhibitor Y 27632, and the p44/42 MAPK inhibitor PD 098059, respectively. (2) Ropivacaine also elicited biphasic changes in [Ca2+]iconsistent with the tension pattern, with a higher force/[Ca2+]ithan that induced by KCl. (3) Ropivacaine induced an enhancement in the phosphorylation of PKC and p44/42 MAPK and membrane translocation of Rho kinase in accord with the contractile response that was each significantly attenuated by bisindolylmaleimide I, calphostin C, Y 27632, and PD 098059.
The biphasic vascular effect is a common characteristic of amino-amide local anesthetic agents that has been well examined in vivo  and in vitro  . Meanwhile, the intensity of vasoconstriction and the concentration required to reach the peak level of contraction are different among this class of drugs.1–8 However, these studies have primarily focused on the contractile phenomenon and their clinical effects. Little is known about their contraction mechanism. Based on the results of the current and other studies, ropivacaine possesses potent and typical biphasic vascular effects and is therefore the object of the current investigation of the molecular mechanisms of amino-amide local anesthetic–induced vasoconstriction.
An increasing body of evidence has elucidated the role of Ca2+-dependent and Ca2+-sensitization mechanisms in mediating smooth muscle contraction, and the involvement of the PKC, Rho-kinase, and p44/42 MAPK signaling pathways in Ca2+sensitization.12–15 During the process of activation, PKC and p44/42 MAPK become phosphorylated,21,22,15 and Rho kinase translocates to the cell membrane.14 Consequently, activation of PKC, p44/42 MAPK, and Rho kinase can be assessed by the detection of PKC and p44/42 MAPK phosphorylation23–25 and Rho-kinase membrane translocation,26,27 respectively. The activated PKC and Rho kinase then primarily phosphorylate the myosin light chain phosphatase to attenuate its activity, thus attenuating the dephosphorylation of phosphorylated myosin light chain 20, and potentiating the activation of actomyosin adenosine triphosphatase and eliciting contraction without affecting [Ca2+]i.13,14 The activated p44/42 MAPK (stimulated mainly by tyrosine kinase, but also partly by PKC) phosphorylates caldesmon and attenuates the inhibition of actomyosin adenosine triphosphatase activity, consequently enhancing smooth muscle contraction, without changing [Ca2+]i.15 The higher force/[Ca2+]iratio in the ropivacaine-induced vascular contraction compared with that in response to KCl in the current study supports the role of mediation of Ca2+sensitivity in the ropivacaine-induced vascular contraction. The roles of PKC, Rho kinase, and p44/42 MAPK in the mediation of the ropivacaine-induced vasoconstriction were demonstrated by the findings of the current study that ropivacaine induced both vascular contraction and phosphorylation of PKC and p44/42 MAPK and membrane translocation of Rho kinase that were inhibited by the respective PKC, Rho-kinase, and p44/42 MAPK inhibitors. The greater extent of the inhibition of the combined inhibitors on the contraction compared with the single inhibitors also suggests that all three of these protein kinase–mediated pathways are involved in the ropivacaine-induced contraction. The efficacy of the inhibitors on the depression of the activities of ropivacaine-induced corresponding kinases was confirmed by the observation of their negligible inhibitory effects on resting tension, but their significant inhibition of the sustained ropivacaine-induced contraction and the ropivacaine-elicited activation of protein kinases.
In the process of regulating smooth muscle contraction, all signaling pathways are not absolutely independent of each other. Some cross-talk or common downstream effectors may exist among these pathways. For example, myosin light chain phosphatase is phosphorylated by both PKC and Rho kinase,13,14 and p44/42 MAPK can be phosphorylated and activated by both PKC28 and tyrosine kinase.29 Therefore, the cross-talk between PKC and p44/42 MAPK may be helpful to explain that the inhibition of contraction by the combination of bisindolylmaleimide I and PD 098059 was less than that of bisindolylmaleimide I and Y 27632 or Y 27632 and PD 098059 in the current study.
The greater extent of the inhibition of the ropivacaine-induced contraction by each of the inhibitors at the highest concentrations used in the current study does not mean that each of the kinase-mediated signaling pathways is capable of dominating the contraction. One of the possible reasons could be the specificity of inhibitors. None of these inhibitors or antagonists can be considered absolutely specific, i.e.  , the higher the concentration is, the relatively lower the specificity is. Therefore, it is possible that the inhibitors also interrupt other signaling cascades at high concentration in addition to their inhibitory effects on their specific targets.
An interesting finding of the current study is that increasing the concentration to higher than 10−3m decreased both the ropivacaine-induced contraction and activation of PKC, Rho kinase, and p44/42 MAPK, indicating that the inhibition of PKC, Rho-kinase, and p44/42 MAPK activation by high concentrations of ropivacaine could at least be one of the causes of the decrease in contraction. However, the mechanism by which the activation of these kinases is limited by high concentrations of ropivacaine could not be explained by the current study. We postulate that ropivacaine at high concentrations could trigger relaxation mechanisms and/or inhibit contraction mechanisms. From another perspective, the current study demonstrated that PKC, Rho kinase, and p44/42 MAPK each contribute as mediators of the ropivacaine-induced contraction. However, whether ropivacaine activates these kinases directly or by activating their upstream effectors, such as tyrosine kinase, or any combination thereof, will require further investigations.
The clinically relevant concentration of ropivacaine is approximately 0.25–0.75% (approximately equivalent to 0.8–2.4 × 10−2m). At this concentration, ropivacaine elicits local blanching by intradermal injection,30 decreases epidural blood flow by epidural injection,31 and reduces cutaneous capillary blood flow by subcutaneous infiltration,32 supporting the impact of ropivacaine on vasoconstriction. However, such concentrations of ropivacaine would not elicit any vascular effect in the current study. We suggest that this discrepancy between our study and others could be caused by several aspects. First, injected ropivacaine may be diluted by interstitial body fluid to concentrations that exert vasoconstriction, although the exact concentration of locally injected ropivacaine has not been measured. Second, the rat aorta used in the current study is a conductance vessel, which primarily functions for conducting blood. Therefore, the aortic contractile response to ropivacaine would likely be different from that in resistant blood vessels and capillaries. The extent of the vasoconstriction produced in the aorta does not always represent the situation of the whole organism in vivo  . Third, the vascular sensitivity to ropivacaine may not be same in different species. Another important cause may be the great differences in experimental conditions between in vivo  and in vitro  studies.
In summary, the findings of the current study demonstrated that ropivacaine induced a dose-dependent biphasic contraction, phosphorylation of PKC and p44/42 MAPK, and membrane translocation of Rho kinase in rat aortic smooth muscle and suggests that PKC, p44/42 MAPK, and Rho kinase are all involved in the ropivacaine-induced vascular contraction.
References
Aps C, Reynolds F: The effect of concentration on vasoactivity of bupivacaine and lignocaine. Br J Anaesth 1976; 48:1171–4Aps, C Reynolds, F
Perlmutter NS, Wilson RA, Edgar SW, Sanders W, Greenberg BH, Tanz R: Vasodilatory effects of lidocaine on epicardial porcine coronary arteries. Pharmacology 1990; 41:280–5Perlmutter, NS Wilson, RA Edgar, SW Sanders, W Greenberg, BH Tanz, R
Johns RA, DiFazio CA, Longnecker DE: Lidocaine constricts or dilates rat arterioles in a dose-dependent manner. Anesthesiology 1985; 62:141–4Johns, RA DiFazio, CA Longnecker, DE
Gherardini G, Samuelson U, Jernbeck J, Aberg B, Sjostrand N: Comparison of vascular effects of ropivacaine and lidocaine on isolated rings of human arteries. Acta Anaesthesiol Scand 1995; 39:765–8Gherardini, G Samuelson, U Jernbeck, J Aberg, B Sjostrand, N
Johns RA, Seyde WC, DiFazio CA, Longnecker DE: Dose-dependent effects of bupivacaine on rat muscle arterioles. Anesthesiology 1986; 65:186–91Johns, RA Seyde, WC DiFazio, CA Longnecker, DE
Newton DJ, McLeod GA, Khan F, Belch JJ: Vasoactive characteristics of bupivacaine and levobupivacaine with and without adjuvant epinephrine in peripheral human skin. Br J Anaesth 2005; 94:662–7Newton, DJ McLeod, GA Khan, F Belch, JJ
Nakamura K, Toda H, Kakuyama M, Nishiwada M, Yamamoto M, Hatano Y, Mori K: Direct vascular effect of ropivacaine in femoral artery and vein of the dog. Acta Anaesthesiol Scand 1993; 37:269–73Nakamura, K Toda, H Kakuyama, M Nishiwada, M Yamamoto, M Hatano, Y Mori, K
Fairley JW, Reynolds F: An intradermal study of the local anaesthetic and vascular effects of the isomers of mepivacaine. Br J Anaesth 1981; 53:1211–6Fairley, JW Reynolds, F
Ishiyama T, Dohi S, Iida H, Watanabe Y: The effects of topical and intravenous ropivacaine on canine pial microcirculation. Anesth Analg 1997; 85:75–81Ishiyama, T Dohi, S Iida, H Watanabe, Y
Iida H, Watanabe Y, Dohi S, Ishiyama T: Direct effects of ropivacaine and bupivacaine on spinal pial vessels in canine: Assessment with closed spinal window technique. Anesthesiology 1997; 87:75–81Iida, H Watanabe, Y Dohi, S Ishiyama, T
Kopacz DJ, Carpenter RL, Mackey DC: Effect of ropivacaine on cutaneous capillary blood flow in pigs. Anesthesiology 1989; 71:69–74Kopacz, DJ Carpenter, RL Mackey, DC
Karaki H, Ozaki H, Hori M, Mitsui-Saito M, Amano K, Harada K, Miyamoto S, Nakazawa H, Won KJ, Sato K: Calcium movements, distribution, and functions in smooth muscle. Pharmacol Rev 1997; 49:157–230Karaki, H Ozaki, H Hori, M Mitsui-Saito, M Amano, K Harada, K Miyamoto, S Nakazawa, H Won, KJ Sato, K
Walsh MP, Horowitz A, Clement-Chomienne O, Andrea JE, Allen BG, Morgan KG: Protein kinase C mediation of Ca2+-independent contractions of vascular smooth muscle. Biochem Cell Biol 1996; 74:485–502Walsh, MP Horowitz, A Clement-Chomienne, O Andrea, JE Allen, BG Morgan, KG
Fukata Y, Amano M, Kaibuchi K: Rho-Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol Sci 2001; 22:32–9Fukata, Y Amano, M Kaibuchi, K
Takahashi E, Berk BC: MAP kinases and vascular smooth muscle function. Acta Physiol Scand 1998; 164:611–21Takahashi, E Berk, BC
Yu J, Ogawa K, Tokinaga Y, Hatano Y: Sevoflurane inhibits GTPγS-stimulated, Rho/Rho-kinase–mediated contraction of isolated rat aortic smooth muscle. Anesthesiology 2003; 99:646–51Yu, J Ogawa, K Tokinaga, Y Hatano, Y
Yu J, Tokinaga Y, Ogawa K, Iwahashi S, Hatano Y: Sevoflurane inhibits angiotensin II–induced, protein kinase C–mediated but not Ca2+-elicited contraction of rat aortic smooth muscle. Anesthesiology 2004; 100:879–84Yu, J Tokinaga, Y Ogawa, K Iwahashi, S Hatano, Y
Yu J, Ogawa K, Tokinaga Y, Mizumoto K, Kakutani T, Hatano Y: The inhibitory effects of isoflurane on protein tyrosine phosphorylation–modulated contraction of rat aortic smooth muscle. Anesthesiology 2004; 101:1325–31Yu, J Ogawa, K Tokinaga, Y Mizumoto, K Kakutani, T Hatano, Y
Molloy CJ, Taylor DS, Weber H: Angiotensin II stimulation of rapid protein tyrosine phosphorylation and protein kinase activation in aortic smooth muscle cells. J Biol Chem 1993; 268:7338–45Molloy, CJ Taylor, DS Weber, H
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein using bicinchoninic acid. Anal Biochem 1985; 150:76–85Smith, PK Krohn, RI Hermanson, GT Mallia, AK Gartner, FH Provenzano, MD Fujimoto, EK Goeke, NM Olson, BJ Klenk, DC
Smith JA, Francis S, Corbin JD: Autophosphorylation: A salient feature of protein kinases. Mol Cell Biochem 1993; 127/128:51–70Smith, JA Francis, S Corbin, JD
Molina CA, Ashendel CL: Tumor promoter 12-O-tetradecanoylphorbol-13-acetate and sn-1,2-dioctanoylglycerol increase the phosphorylation of protein kinase C in cells. Cancer Res 1991; 51:4624–30Molina, CA Ashendel, CL
Mitchell FE, Marais RM, Parker PJ: The phosphorylation of protein kinase C as a potential measure of activation. Biochem J 1989; 261:131–6Mitchell, FE Marais, RM Parker, PJ
Duff JL, Berk BC, Corson MA: Angiotensin II stimulates the pp44 and pp42 mitogen-activated protein kinases in cultured rat aortic smooth muscle cells. Biochem Biophys Res Commun 1992; 188:257–64Duff, JL Berk, BC Corson, MA
Ratz PH: Regulation of ERK phosphorylation in differentiated arterial muscle of rabbits. Am J Physiol 2001; 281:H114–23Ratz, PH
Gong MC, Gorenne I, Read P, Jia T, Nakamoto RK, Somlyo AV, Somlyo AP: Regulation by GDI of Rho/Rho-kinase-induced Ca2+sensitization of smooth muscle myosin II. Am J Physiol Cell Physiol 2001; 281:C257–69Gong, MC Gorenne, I Read, P Jia, T Nakamoto, RK Somlyo, AV Somlyo, AP
Carter RW, Begaye M, Kanagy NL: Acute and chronic NOS inhibition enhances α2-adrenoreceptor-stimulated RhoA and Rho kinase in rat aorta. Am J Physiol Heart Circ Physiol 2002; 283:H1361–9Carter, RW Begaye, M Kanagy, NL
Liao DF, Monia B, Dean N, Berk BC: Protein kinase C-zeta mediates angiotensin II activation of ERK1/2 in vascular smooth muscle cells. J Biol Chem 1997; 272:6146–50Liao, DF Monia, B Dean, N Berk, BC
Puri RN, Fan YP, Rattan S: Role of pp60 c-src  and p44/42 MAPK in ANG II-induced contraction of rat tonic gastrointestinal smooth muscles. Am J Physiol 2002; 283:G390–9Puri, RN Fan, YP Rattan, S
Cederholm I, Akerman B, Evers H: Local analgesic and vascular effects of intradermal ropivacaine and bupivacaine in various concentrations with and without addition of adrenaline in man. Acta Anaesthesiol Scand 1994; 38:322–7Cederholm, I Akerman, B Evers, H
Dahl JB, Simonsen L, Mogensen T, Henriksen JH, Kehlet H: The effect of 0.5% ropivacaine on epidural blood flow. Acta Anaesthesiol Scand 1990; 34:308–10Dahl, JB Simonsen, L Mogensen, T Henriksen, JH Kehlet, H
Kopacz DJ, Carpenter RL, Mackey DC: Effect of ropivacaine on cutaneous capillary blood flow in pigs. Anesthesiology 1989; 71:69–74Kopacz, DJ Carpenter, RL Mackey, DC
Fig. 1. Amino-amide local anesthetic agent–induced, dose-dependent, biphasic contraction of rat aortic smooth muscle. Ropivacaine, bupivacaine, mepivacaine, and lidocaine were delivered to the endothelium-denuded rings in a cumulative dose manner. The resultant tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride (KCl)–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  lidocaine. n = 6 (  A  ). A recording of the ropivacaine-induced contraction was presented (  B  ). 
Fig. 1. Amino-amide local anesthetic agent–induced, dose-dependent, biphasic contraction of rat aortic smooth muscle. Ropivacaine, bupivacaine, mepivacaine, and lidocaine were delivered to the endothelium-denuded rings in a cumulative dose manner. The resultant tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride (KCl)–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  lidocaine. n = 6 (  A  ). A recording of the ropivacaine-induced contraction was presented (  B  ). 
Fig. 1. Amino-amide local anesthetic agent–induced, dose-dependent, biphasic contraction of rat aortic smooth muscle. Ropivacaine, bupivacaine, mepivacaine, and lidocaine were delivered to the endothelium-denuded rings in a cumulative dose manner. The resultant tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride (KCl)–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  lidocaine. n = 6 (  A  ). A recording of the ropivacaine-induced contraction was presented (  B  ). 
×
Fig. 2. Ropivacaine (Rop)–induced, dose-dependent, biphasic increase in intracellular calcium concentration ([Ca2+]i) of rat aortic smooth muscle. The [Ca2+]iof fura-2–loaded aortic strips was detected using fluorometer and was expressed as the ratio F340/F380 (  A  ). The [Ca2+]ilevels stimulated by ropivacaine are presented as a percent of the 30 mm potassium chloride (KCl)–induced value (  B  ). *  P  < 0.05, **  P  < 0.01  versus  baseline (n = 6). 
Fig. 2. Ropivacaine (Rop)–induced, dose-dependent, biphasic increase in intracellular calcium concentration ([Ca2+]i) of rat aortic smooth muscle. The [Ca2+]iof fura-2–loaded aortic strips was detected using fluorometer and was expressed as the ratio F340/F380 (  A  ). The [Ca2+]ilevels stimulated by ropivacaine are presented as a percent of the 30 mm potassium chloride (KCl)–induced value (  B  ). *  P  < 0.05, **  P  < 0.01  versus  baseline (n = 6). 
Fig. 2. Ropivacaine (Rop)–induced, dose-dependent, biphasic increase in intracellular calcium concentration ([Ca2+]i) of rat aortic smooth muscle. The [Ca2+]iof fura-2–loaded aortic strips was detected using fluorometer and was expressed as the ratio F340/F380 (  A  ). The [Ca2+]ilevels stimulated by ropivacaine are presented as a percent of the 30 mm potassium chloride (KCl)–induced value (  B  ). *  P  < 0.05, **  P  < 0.01  versus  baseline (n = 6). 
×
Fig. 3. Effects of the protein kinase inhibitors on the resting tension (  A  and  B  ) and ropivacaine (Rop)–induced sustained contraction (  C  and  D  ) of rat aortic smooth muscle. The tension was measured using isometric force transducers. Endothelium-denuded rings were equilibrated for 1 h at a resting tension of 3 g and then exposed to different concentrations of bisindolylmaleimide I (Bis I) for 15 min (  A  ). The statistical data for the effects of all of the inhibitors used on resting tension are presented in  B  . The resting tension (3 g) was considered as 100% (n = 6). A bolus of ropivacaine (3 × 10−4m) was applied to the rings to induce a sustained contraction, and Bis I was then delivered (  C  ). The statistical data for the effects of all of the inhibitors used on sustained ropivacaine-induced contraction are presented in  D  . Ropivacaine-induced contraction was expressed as a percent of the 30 mm potassium chloride–induced contraction. **  P  < 0.01  versus  control (n = 6). Calp C = calphostin C; PD = PD 098059; Y = Y 27632. 
Fig. 3. Effects of the protein kinase inhibitors on the resting tension (  A  and  B  ) and ropivacaine (Rop)–induced sustained contraction (  C  and  D  ) of rat aortic smooth muscle. The tension was measured using isometric force transducers. Endothelium-denuded rings were equilibrated for 1 h at a resting tension of 3 g and then exposed to different concentrations of bisindolylmaleimide I (Bis I) for 15 min (  A  ). The statistical data for the effects of all of the inhibitors used on resting tension are presented in  B  . The resting tension (3 g) was considered as 100% (n = 6). A bolus of ropivacaine (3 × 10−4m) was applied to the rings to induce a sustained contraction, and Bis I was then delivered (  C  ). The statistical data for the effects of all of the inhibitors used on sustained ropivacaine-induced contraction are presented in  D  . Ropivacaine-induced contraction was expressed as a percent of the 30 mm potassium chloride–induced contraction. **  P  < 0.01  versus  control (n = 6). Calp C = calphostin C; PD = PD 098059; Y = Y 27632. 
Fig. 3. Effects of the protein kinase inhibitors on the resting tension (  A  and  B  ) and ropivacaine (Rop)–induced sustained contraction (  C  and  D  ) of rat aortic smooth muscle. The tension was measured using isometric force transducers. Endothelium-denuded rings were equilibrated for 1 h at a resting tension of 3 g and then exposed to different concentrations of bisindolylmaleimide I (Bis I) for 15 min (  A  ). The statistical data for the effects of all of the inhibitors used on resting tension are presented in  B  . The resting tension (3 g) was considered as 100% (n = 6). A bolus of ropivacaine (3 × 10−4m) was applied to the rings to induce a sustained contraction, and Bis I was then delivered (  C  ). The statistical data for the effects of all of the inhibitors used on sustained ropivacaine-induced contraction are presented in  D  . Ropivacaine-induced contraction was expressed as a percent of the 30 mm potassium chloride–induced contraction. **  P  < 0.01  versus  control (n = 6). Calp C = calphostin C; PD = PD 098059; Y = Y 27632. 
×
Fig. 4. Effects of the protein kinase C inhibitor bisindolylmaleimide I (Bis I;  A  ), calphostin C (Calp C;  B  ), the Rho-kinase inhibitor Y 27632 (Y;  C  ), and the p44/42 MAPK inhibitor PD 098059 (PD;  D  ) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with the above-mentioned inhibitors at different concentrations for 15 min and were then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 4. Effects of the protein kinase C inhibitor bisindolylmaleimide I (Bis I;  A  ), calphostin C (Calp C;  B  ), the Rho-kinase inhibitor Y 27632 (Y;  C  ), and the p44/42 MAPK inhibitor PD 098059 (PD;  D  ) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with the above-mentioned inhibitors at different concentrations for 15 min and were then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 4. Effects of the protein kinase C inhibitor bisindolylmaleimide I (Bis I;  A  ), calphostin C (Calp C;  B  ), the Rho-kinase inhibitor Y 27632 (Y;  C  ), and the p44/42 MAPK inhibitor PD 098059 (PD;  D  ) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with the above-mentioned inhibitors at different concentrations for 15 min and were then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
×
Fig. 5. Effects of combining bisindolylmaleimide I (Bis I; 10−6m), Y 27632 (Y; 5 × 10−7m), and PD 098059 (PD; 10−5m) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with one of the four combinations for 15 min and then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 5. Effects of combining bisindolylmaleimide I (Bis I; 10−6m), Y 27632 (Y; 5 × 10−7m), and PD 098059 (PD; 10−5m) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with one of the four combinations for 15 min and then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
Fig. 5. Effects of combining bisindolylmaleimide I (Bis I; 10−6m), Y 27632 (Y; 5 × 10−7m), and PD 098059 (PD; 10−5m) on the ropivacaine (Rop)–induced, dose-dependent, cumulative contraction of rat aortic smooth muscle. Endothelium-denuded rings were randomly pretreated with one of the four combinations for 15 min and then challenged by ropivacaine in a cumulative dose manner. The tension was measured using isometric force transducers and was expressed as a percent of the 30 mm potassium chloride–induced contraction. *  P  < 0.05, **  P  < 0.01  versus  control (n = 6). 
×
Fig. 6. Ropivacaine (Rop)–induced phosphorylation of protein kinase C (PKC) and p44/42 mitogen-activated protein kinase (p44/42 MAPK) and Rho-kinase membrane translocation in rat aortic smooth muscle. Endothelium-denuded strips were randomly challenged by different concentrations of ropivacaine for 20 min or were first pretreated with bisindolylmaleimide I (Bis I; 10−5m) (  A  ), calphostin C (Calp C; 10−6m) (  B  ), Y 27632 (Y; 5 × 10−6m) (  C  ), or PD 098059 (PD; 10−4m) (  D  ) for 15 min and then challenged with ropivacaine (3 × 10−4m) for 20 min. PKC and p44/42 MAPK phosphorylation and Rho-kinase (Rock-2) membrane translocation were detected using Western blotting with specific antibodies. Values for phosphorylation of PKC and p44/42 MAPK were expressed as percentages of the total PKC and total p44/42 MAPK, respectively. The level of Rock-2 in the membrane fraction was expressed as a percentage of the total Rock-2 (  i.e.  , membrane fraction plus cytosolic fraction). *  P  < 0.05,  P  < 0.01  versus  control (0 m) (n = 4). C = cytosolic fraction; M = membrane fraction; p-p44/42 MAPK = phosphorylated p44/42 mitogen-activated protein kinase; p-PKC = phosphorylated protein kinase C. 
Fig. 6. Ropivacaine (Rop)–induced phosphorylation of protein kinase C (PKC) and p44/42 mitogen-activated protein kinase (p44/42 MAPK) and Rho-kinase membrane translocation in rat aortic smooth muscle. Endothelium-denuded strips were randomly challenged by different concentrations of ropivacaine for 20 min or were first pretreated with bisindolylmaleimide I (Bis I; 10−5m) (  A  ), calphostin C (Calp C; 10−6m) (  B  ), Y 27632 (Y; 5 × 10−6m) (  C  ), or PD 098059 (PD; 10−4m) (  D  ) for 15 min and then challenged with ropivacaine (3 × 10−4m) for 20 min. PKC and p44/42 MAPK phosphorylation and Rho-kinase (Rock-2) membrane translocation were detected using Western blotting with specific antibodies. Values for phosphorylation of PKC and p44/42 MAPK were expressed as percentages of the total PKC and total p44/42 MAPK, respectively. The level of Rock-2 in the membrane fraction was expressed as a percentage of the total Rock-2 (  i.e.  , membrane fraction plus cytosolic fraction). *  P  < 0.05,  P  < 0.01  versus  control (0 m) (n = 4). C = cytosolic fraction; M = membrane fraction; p-p44/42 MAPK = phosphorylated p44/42 mitogen-activated protein kinase; p-PKC = phosphorylated protein kinase C. 
Fig. 6. Ropivacaine (Rop)–induced phosphorylation of protein kinase C (PKC) and p44/42 mitogen-activated protein kinase (p44/42 MAPK) and Rho-kinase membrane translocation in rat aortic smooth muscle. Endothelium-denuded strips were randomly challenged by different concentrations of ropivacaine for 20 min or were first pretreated with bisindolylmaleimide I (Bis I; 10−5m) (  A  ), calphostin C (Calp C; 10−6m) (  B  ), Y 27632 (Y; 5 × 10−6m) (  C  ), or PD 098059 (PD; 10−4m) (  D  ) for 15 min and then challenged with ropivacaine (3 × 10−4m) for 20 min. PKC and p44/42 MAPK phosphorylation and Rho-kinase (Rock-2) membrane translocation were detected using Western blotting with specific antibodies. Values for phosphorylation of PKC and p44/42 MAPK were expressed as percentages of the total PKC and total p44/42 MAPK, respectively. The level of Rock-2 in the membrane fraction was expressed as a percentage of the total Rock-2 (  i.e.  , membrane fraction plus cytosolic fraction). *  P  < 0.05,  P  < 0.01  versus  control (0 m) (n = 4). C = cytosolic fraction; M = membrane fraction; p-p44/42 MAPK = phosphorylated p44/42 mitogen-activated protein kinase; p-PKC = phosphorylated protein kinase C. 
×