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Meeting Abstracts  |   October 1997
Bupivacaine Inhibition of L-Type Calcium Current in Ventricular Cardiomyocytes of Hamster 
Author Notes
  • (Rossner) Research Assistant Professor of Anesthesiology, State University of New York at Stony Brook.
  • (Freese) Professor of Clinical Anesthesiology, State University of New York at Stony Brook.
  • Received from the Departments of Anesthesiology, Winthrop-University Hospital, Mineola, New York, and Nassau County Medical Center, East Meadow, New York. Submitted for publication February 14, 1997. Accepted for publication June 19, 1997. The work was performed in the Anesthesiology Research Laboratory, Department of Anesthesiology, Nassau County Medical Center, East Meadow, New York. Supported, in part, by the Meadowbrook Medical Education and Research Foundation, Inc., East Meadow, New York. Presented in preliminary form at the American Society of Anesthesiologists 1995 Annual Meeting, Atlanta, Georgia, October 21–25, 1995.
  • Address reprint requests to Dr. Rossner: Department of Anesthesiology, Nassau County Medical Center, 2201 Hempstead Turnpike, East Meadow, New York 11554. Address electronic mail to: krossner@epo.som.sunysb.edu.
Article Information
Meeting Abstracts   |   October 1997
Bupivacaine Inhibition of L-Type Calcium Current in Ventricular Cardiomyocytes of Hamster 
Anesthesiology 10 1997, Vol.87, 926-934. doi:
Anesthesiology 10 1997, Vol.87, 926-934. doi:
When bupivacaine is accidentally injected into the circulation, cardiovascular complications such as dysrhythmias and cardiac arrest may ensue. [1 ] Total blood concentrations of bupivacaine in patients with these complications typically range from 2–4 micro gram/ml. [2 ] Cardiodepressant effects of bupivacaine are seen in vitro by reductions in contraction force of heart tissue. [3–7 ] A determinant of the cardiodepression might be an inhibition of calcium current through L-type Ca2+ channels (ICa,L). Electrophysiologic studies have examined the effect of bupivacaine on I sub Ca,L in rat sensory neurons [8 ] and in cardiac cells using calcium-dependent action potentials in myocardium of guinea pig, [6,9,10 ] double sucrose gap voltage clamp of frog atrial tissue, [11 ] and whole-cell patch clamp of isolated ventricular cardiomyocytes of guinea pig. [9,12 ] All report that bupivacaine inhibits the current, but few details are available that disclose the mechanism of inhibition. With this in mind, we have studied certain characteristics of the bupivacaine inhibition of ICa,L, such as concentration-response, steady-state inactivation, inactivation time constants, time course of recovery from inactivation, and voltage dependency.
Methods and Materials 
Preparation of Cardiomyocytes 
Cardiomyocytes were isolated from the left ventricular free wall of hearts of 16 normal male hamsters (aged 92 to 199 days) by a protocol approved by the Institutional Animal Care and Use Committee of Nassau County Medical Center. Hamsters, BIO FIB strain (BIO-Breeders, Inc., Watertown, MA), were anesthetized by intraperitoneal injection of pentobarbital sodium (60 mg/kg). When corneal and footpad-withdrawal reflexes were absent the anesthetized hamster was killed by cervical dislocation. The heart was quickly removed intact with a small length of aorta. Cardiomyocytes were isolated by an enzymatic dispersion method slightly modified from that described elsewhere. [13 ] Briefly, the aorta was cannulated on a Langendorff column and perfused for 15 min with nominally Ca2+-free (10–20 micro Meter Ca2+) modified Tyrode solution that contained (mM) 135 NaCl, 5.4 KCl, 1 MgCl2, 10 HEPES (N-[2-Hydroxyethyl]piperazine-N'-[2-ethanesulfonic acid], 0.33 NaH sub 2 PO4, 10 glucose (pH 7.83–7.39 with NaOH). Perfusion with an enzyme solution, containing 0.09% collagenase (Boehringer Mannheim, type B), and from 0.0047 to 0.019% protease (Sigma, type XIV) mixed in nominally Ca2+-free Tyrode solution, followed for 15–20 min. The softened heart was perfused for 4–5 min with a modified “KB medium”[14 ] that contained (mM) 30 KCl, 30 KH2PO4, 3 MgSO4, 50 glutamate, 20 taurine, 10 glucose, 0.5 EGTA (Ethylene Glycol-bis[b-Aminoethyl Ether]N,N,N',N'-Tetraacetic Acid), 20 HEPES (pH 7.3 with KOH). All perfusates were heated to 37 [degree sign] Celsius and oxygenated (100% O2). The heart was removed from the column, and the left ventricular free wall was dissected in modified KB solution. Gentle agitation with forceps dispersed the cells. Filtration through a 200-micro meter nylon mesh (Tetko Inc., Elmsford, NY) separated individual cardiomyocytes from tissue clumps. Myocytes were incubated for a minimum of 1 h in modified KB solution at room temperature and stored at 10 [degree sign] Celsius for use during the same day. Approximately 80–90% of the myocytes survived in modified KB solution. Survival was appraised by the retention of rod shape and distinct sarcomere striations. In contrast, dead cells were rounded and nonstriated. On reexposure to modified Tyrode solution that contained 1.8 mM CaCl2, the percentage of surviving cells decreased to 40–50%. Electrophysiology was performed on cardiomyocytes that were relaxed, rod-shaped, and distinctly striated.
Electrophysiology 
Patch microelectrodes were prepared from filamented glass tubing (1.5 mm outer diameter [OD], borosilicate, Sutter Instruments Co., Novato, CA). Tips were heat polished and filled with a solution that contained (mM) 125 cesium aspartate, 25 tetraethylammonium chloride (TEA), 1 MgCl2, 5 HEPES, 10 EGTA, and 5 MgATP. The pH was adjusted to 7.30 with CsOH. The Cs sup + and TEA minimized potassium currents. Cardiomyocytes were bathed in modified Tyrode solution with 1.8 mM CaCl sub 2 added. In experiments that used Ba2+ as the charge carrier, 1.8 mM BaCl2replaced CaCl2. The Tyrode solution was maintained at 34.7–35.3 [degree sign] Celsius in a 150-micro liter volume chamber (PDMI-2 Open Perfusion Micro-Incubator, Medical Systems Corp., Greenvale, NY) that was mounted on the stage of an inverted microscope (Diaphot, Nikon Inc., Melville, NY). The superfusion of Tyrode solution, at a rate of 4 ml/min, was continuous throughout the experiment. Electrode tip resistances ranged from 4.2–8.1 M Omega. Tip potentials were adjusted to 0 mV before high-resistance seals were established with the sarcolemma of myocytes (1–10 G Omega). Rupturing the patched membrane with further suction established the whole-cell recording. Normally, the holding potential was set to -40 mV. After a minimum of 10 min to allow the holding current to stabilize between -0.1 and -0.15 nA, ICa,L was evoked with positive potential command steps. The holding potential of -40 mV was sufficient to fully inactivate voltage-sensitive Na channels and to eliminate current through T-type Ca channels, which were not studied. In some experiments, the holding potential was set to -50 or -20 mV. When the holding potential was -50 mV, 10 micro Meter tetrodotoxin (TTX) was added to the bath, a concentration that proved sufficient to fully block INa. This was tested by adding CdCl2(0.2 mM) to the bath. Cd2+, which specifically inhibits ICa,L, completely blocked all inward currents, thereby showing that TTX inhibited INa. The holding potentials and command potential amplitudes, durations, and frequencies were determined by Clampex software (pClamp6, Axon Instruments, Inc., Foster City, CA), which was run on a Gateway 2000 computer. The computer governed the Axopatch 200A amplifier (Axon Instruments, Inc.) that was outfitted with a CV201A headstage. Current signals, partially corrected for series resistance and whole-cell capacitance, were filtered online at 5 kHz (-3 dB) and stored on hard disk for later analysis by Clampfit software (pClamp6, Axon Instruments, Inc.). The amplitude of ICa,L was measured from 0 nA. However, in experiments designed to determine current versus voltage and steady-state inactivation, the current amplitude was measured as the difference between the peak inward current and the steady-state current near the end of the 200-ms current signal. Bupivacaine HCl (Sigma Chemical Co.) was prepared to varying concentrations in modified Tyrode solution and was administered by superfusion.
To determine whether ATP in the dialyzing pipette solution altered the sensitivity of myocytes to bupivacaine, the response of I sub Ca,L to 30 micro Meter bupivacaine was measured in the absence of ATP. The presence or absence of ATP had no effect.
Rundown, or the spontaneous decrease in ICa,L amplitude, was linear with time and did not exceed 0.012 nA/min in bupivacaine-free conditions with ATP in the pipette solution. The formula used to correct for rundown was: Icorr. =(m [center dot] t)+ It, where Icorr. = peak ICa,L corrected for rundown (nA), m = rundown slope (na [center dot] s sup -1), t = elapsed time (s), and It= peak I sub Ca,L at time t (nA).
Statistical Analysis 
Curves were fit to plotted data using the Marquardt-Levenberg algorithm available in SigmaPlot (Jandel Corp.). Coefficients of determination (R2) appraised the correctness of fits. Student's t test for paired samples was used to compare control versus bupivacaine when one concentration of bupivacaine was applied to the myocyte (e.g., concentration-response data). Where noted, Student's t test for independent samples was used. When a myocyte was exposed to two concentrations of bupivacaine and washout, multiple pairwise comparisons of data were done with Bonferroni's method. Statistical significance was assumed at P < 0.05. Data are presented as mean +/- SEM.
Results 
Concentration-Response 
The peak amplitude of ICa,L was decreased by bupivacaine (Figure 1, Figure 2). The extent of inhibition that was achieved by each concentration appeared to stabilize by 100 s of application (Figure 2). A wash effect from 10 micro Meter was not seen. Two of the six cells studied in the wash from 10 micro Meter exhibited an inexplicable rapid decay in ICa,L without a concomitant change in holding current; the decay, therefore, did not appear to be the result of a loss of recording integrity (e.g., seal resistance). The rapid wash-related decay of ICa,L was not seen for any of the other 16 cells during wash from higher concentrations. Wash only partly, but rapidly, restored the control amplitudes of ICa,L in 30, 100, and 300 micro Meter. Wash from these higher concentrations restored ICa,L only to the levels of inhibition approximating that seen in 10 micro Meter of bupivacaine. In Figure 2, the rates of inhibition and washout appeared to accelerate with increasing concentrations of bupivacaine. Single exponential fits of the average data (R2> 0.99) confirmed this impression. Time constants of inhibition were 47, 46, 21, and 15 s for 10, 30, 100, and 300 micro Meter, respectively. Time constants of washout were 48, 49, and 24 s for 30, 100, and 300 micro Meter, respectively. A concentration-response curve (Figure 3) was constructed from the data of Figure 2and was used to estimate the concentration that evoked 50% inhibition (IC50). The average value of IC50was 112.0 +/- 9.1 micro Meter.
Figure 1. Representative current traces with (A) Ca2+, and (B) Ba sup 2+ as charge carriers. Currents were evoked by 200 ms (A) and 300 ms (B) command steps to +5 mV (0.2 Hz stimulus frequency) from a holding potential of -40 mV. Dashed lines are fits of the data for determining the two time constants of inactivation by the Simplex least squares fitting method available in Clampfit (Table 1, Table 2). In (A), the two traces show the control and response to 100 micro Meter bupivacaine (2 min) from the same myocyte. Bupivacaine brought the holding current closer to 0 nA. In (B), the two traces of IBa,L show the control and response to 30 micro Meter bupivacaine (2 min) from the same myocyte.
Figure 1. Representative current traces with (A) Ca2+, and (B) Ba sup 2+ as charge carriers. Currents were evoked by 200 ms (A) and 300 ms (B) command steps to +5 mV (0.2 Hz stimulus frequency) from a holding potential of -40 mV. Dashed lines are fits of the data for determining the two time constants of inactivation by the Simplex least squares fitting method available in Clampfit (Table 1, Table 2). In (A), the two traces show the control and response to 100 micro Meter bupivacaine (2 min) from the same myocyte. Bupivacaine brought the holding current closer to 0 nA. In (B), the two traces of IBa,L show the control and response to 30 micro Meter bupivacaine (2 min) from the same myocyte.
Figure 1. Representative current traces with (A) Ca2+, and (B) Ba sup 2+ as charge carriers. Currents were evoked by 200 ms (A) and 300 ms (B) command steps to +5 mV (0.2 Hz stimulus frequency) from a holding potential of -40 mV. Dashed lines are fits of the data for determining the two time constants of inactivation by the Simplex least squares fitting method available in Clampfit (Table 1, Table 2). In (A), the two traces show the control and response to 100 micro Meter bupivacaine (2 min) from the same myocyte. Bupivacaine brought the holding current closer to 0 nA. In (B), the two traces of IBa,L show the control and response to 30 micro Meter bupivacaine (2 min) from the same myocyte.
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Figure 2. Inhibition of ICa,L by four concentrations of bupivacaine. Ordinates are fractions of peak ICa,L (i.e., ICa,l /ICa,L control) after correction for rundown, which did not exceed 0.012 nA/min. Currents were evoked as in Figure 1(A). Five minutes of control (open circles) was followed by 5 min of bupivacaine (filled circles, onset at upward arrow), and 5 min of wash (open circles, onset at downward arrow). N = 6 (10 micro Meter), 5 (30 micro Meter), 5 (100 micro Meter), and 6 (300 micro Meter) myocytes. SEM bars were not shown (see Figure 3for SEM bars).
Figure 2. Inhibition of ICa,L by four concentrations of bupivacaine. Ordinates are fractions of peak ICa,L (i.e., ICa,l /ICa,L control) after correction for rundown, which did not exceed 0.012 nA/min. Currents were evoked as in Figure 1(A). Five minutes of control (open circles) was followed by 5 min of bupivacaine (filled circles, onset at upward arrow), and 5 min of wash (open circles, onset at downward arrow). N = 6 (10 micro Meter), 5 (30 micro Meter), 5 (100 micro Meter), and 6 (300 micro Meter) myocytes. SEM bars were not shown (see Figure 3for SEM bars).
Figure 2. Inhibition of ICa,L by four concentrations of bupivacaine. Ordinates are fractions of peak ICa,L (i.e., ICa,l /ICa,L control) after correction for rundown, which did not exceed 0.012 nA/min. Currents were evoked as in Figure 1(A). Five minutes of control (open circles) was followed by 5 min of bupivacaine (filled circles, onset at upward arrow), and 5 min of wash (open circles, onset at downward arrow). N = 6 (10 micro Meter), 5 (30 micro Meter), 5 (100 micro Meter), and 6 (300 micro Meter) myocytes. SEM bars were not shown (see Figure 3for SEM bars).
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Figure 3. Inhibition of peak ICa,L versus bupivacaine concentration. Data are measured from the same cells used in Figure 2, and peak currents were measured at 5 min of drug application. Extents of inhibition (Delta % of control) are given. The data points were fit (solid lines) to a logistic function (inset) for estimating IC50, where y and x are ordinate and abscissa values, respectively. IC50= 112.0 +/- 9.1 micro Meter, and the slope factor n =-0.9881 +/- 0.008 micro Meter. The filled circles are larger than the SEM bars for 10 and 300 micro Meter. The size of SEM bars are the same as for the data point averages of Figure 2.
Figure 3. Inhibition of peak ICa,L versus bupivacaine concentration. Data are measured from the same cells used in Figure 2, and peak currents were measured at 5 min of drug application. Extents of inhibition (Delta % of control) are given. The data points were fit (solid lines) to a logistic function (inset) for estimating IC50, where y and x are ordinate and abscissa values, respectively. IC50= 112.0 +/- 9.1 micro Meter, and the slope factor n =-0.9881 +/- 0.008 micro Meter. The filled circles are larger than the SEM bars for 10 and 300 micro Meter. The size of SEM bars are the same as for the data point averages of Figure 2.
Figure 3. Inhibition of peak ICa,L versus bupivacaine concentration. Data are measured from the same cells used in Figure 2, and peak currents were measured at 5 min of drug application. Extents of inhibition (Delta % of control) are given. The data points were fit (solid lines) to a logistic function (inset) for estimating IC50, where y and x are ordinate and abscissa values, respectively. IC50= 112.0 +/- 9.1 micro Meter, and the slope factor n =-0.9881 +/- 0.008 micro Meter. The filled circles are larger than the SEM bars for 10 and 300 micro Meter. The size of SEM bars are the same as for the data point averages of Figure 2.
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Table 1. Time Constants of Inactivation in Different Concentrations of Bupivacaine HCl 
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Table 1. Time Constants of Inactivation in Different Concentrations of Bupivacaine HCl 
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Table 2. Time Constants of Inactivation with Ba2+ as the Charge Carrier 
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Table 2. Time Constants of Inactivation with Ba2+ as the Charge Carrier 
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Current-Voltage Relationship 
The relationship of ICa,L to the amplitude of stimulus command voltage (I vs. V) is shown in Figure 4. Bupivacaine (10 and 100 micro Meter) significantly decreased the amplitude of ICa,L, but did not alter the overall shape of the I versus V relationship, except at -30 and -20 mV, wherein the activation component appeared to be depressed by bupivacaine and could not be reversed with wash.
Figure 4. Current versus voltage relationship. Averages of peak amplitudes of ICa,L were plotted against command voltages. Records were obtained at 2 to 2.5 min of exposure to each concentration, or 2 to 2.5 min of wash. Data are from five myocytes. ** denotes P < 0.05 for control versus 10 micro Meter, 100 micro Meter, and wash, respectively. * denotes P < 0.05 for control versus 100 micro Meter and wash, respectively (i.e., P > 0.05 for control vs. 10 micro Meter).
Figure 4. Current versus voltage relationship. Averages of peak amplitudes of ICa,L were plotted against command voltages. Records were obtained at 2 to 2.5 min of exposure to each concentration, or 2 to 2.5 min of wash. Data are from five myocytes. ** denotes P < 0.05 for control versus 10 micro Meter, 100 micro Meter, and wash, respectively. * denotes P < 0.05 for control versus 100 micro Meter and wash, respectively (i.e., P > 0.05 for control vs. 10 micro Meter).
Figure 4. Current versus voltage relationship. Averages of peak amplitudes of ICa,L were plotted against command voltages. Records were obtained at 2 to 2.5 min of exposure to each concentration, or 2 to 2.5 min of wash. Data are from five myocytes. ** denotes P < 0.05 for control versus 10 micro Meter, 100 micro Meter, and wash, respectively. * denotes P < 0.05 for control versus 100 micro Meter and wash, respectively (i.e., P > 0.05 for control vs. 10 micro Meter).
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Time to Activation and Rates of Inactivation 
The time to activation of ICa,L was appraised by the time between the start of stimulation and the current peak (time-to-peak [TTP]) from the same cells used in the concentration-response study (Figure 1(A), Figure 2, and Figure 3). TTP increased slightly, but significantly, in all concentrations of bupivacaine (10, 30, 100, and 300 micro Meter). Because the increase was dose-independent (P > 0.19 by Student's t test for independent samples), we report only the average TTP for control and bupivacaine records. These were 7.8 +/- 0.2 ms (n = 22) for control and 9.0 +/- 0.3 ms (n = 22) for bupivacaine (P < 0.001). The average increase in TTP was 1.1 +/- 0.2 ms above the control value. Wash (5 min) did not restore TTP to the control level. When Ba2+ was the charge carrier, the increase in TTP in 30 micro Meter bupivacaine was not statistically significant from control (P > 0.10). The values of TTP were 8.5 +/- 0.4 ms (n = 5) for control and 9.3 +/- 0.6 ms for 30 micro Meter bupivacaine. Also, there was no significant difference between the control and wash averages of TTP. The durations of capacitative transients were from 1.44 to 3.12 ms and for each cell did not change by more than 0.36 ms during an experiment (control-bupivacaine-washout). Therefore, it is likely that measurements of TTP were not significantly distorted by the capacitative transients that persisted after partial correction.
Inactivation, or the spontaneous decay of ICa,L, was fit to a biexponential model to extract two time constants of inactivation (tauf1and tauf2). These are reported in Table 1for those cells used in the concentration-response study (Figure 1(A), Figure 2, and Figure 3). There was a slight, but significant, decrease in tauf1in 30 micro Meter, and tauf2significantly increased in 100 micro Meter. The general impression was that the rates of inactivation of ICa,L were not substantively changed from control values. However, when Ba2+ was the charge carrier (Figure 1(B)), tauf1and tauf2were significantly reduced by 30 micro Meter bupivacaine (Table 2). Bupivacaine (30 micro Meter) inhibited the peak amplitude of IBa,L by 29.6 +/- 2.4% of control. The extent of inhibition was not significantly different (P = 0.07; Student's t test for independent samples) from that seen for ICa,L in 30 micro Meter bupivacaine, 21.1 +/- 3.2%(Figure 3).
Steady-state Inactivation 
Bupivacaine (10 and 100 micro Meter) shifted the steady-state inactivation (f [infinity]) curve leftward to negative potentials (Figure 5). The membrane voltage at which the fraction of maximum I sub Ca,L was reduced to one-half (V0.5) indexed the shift. In other words, V0.5 is the membrane voltage at which 50% of the Ca channels are in the inactivated state (i.e., unavailable to open). The negative shift was concentration-dependent, and wash did not restore V0.5 to control levels (Table 3). In a time control, V0.5 did not change more than 0.018 mV, strengthening the conclusion that the negative shift of the f [infinity] curve was caused by bupivacaine. For two myocytes, the f [infinity] curves were begun with a conditioning potential of -50 mV (data not shown). Under control conditions, the extent of inactivation of ICa,L was identical at -50 and -40 mV, i.e., the fraction of maximum peak ICa,L equaled 1 at -50 and -40 mV. This result justified using -40 mV as the starting conditioning potential for the bulk of f [infinity] experiments.
Figure 5. Steady-state inactivation (f [infinity]). The schematic depicts the stimulus protocol, which was repeated every 5 s. Variable amplitude conditioning potentials (C) were followed by a test potential (T). Amplitudes of C (shown only to -10 mV) were extended to +10 mV. As C was increased, the amplitude of ICa,L evoked by T decreased. The fraction of maximum ICa,L evoked by T was plotted against the amplitude of C. Estimates of V0.5 and slope factor (k) were obtained using the Boltzmann function (inset), and are given in Table 3. Data are from six myocytes. R2for fits were not less than 0.998.
Figure 5. Steady-state inactivation (f [infinity]). The schematic depicts the stimulus protocol, which was repeated every 5 s. Variable amplitude conditioning potentials (C) were followed by a test potential (T). Amplitudes of C (shown only to -10 mV) were extended to +10 mV. As C was increased, the amplitude of ICa,L evoked by T decreased. The fraction of maximum ICa,L evoked by T was plotted against the amplitude of C. Estimates of V0.5 and slope factor (k) were obtained using the Boltzmann function (inset), and are given in Table 3. Data are from six myocytes. R2for fits were not less than 0.998.
Figure 5. Steady-state inactivation (f [infinity]). The schematic depicts the stimulus protocol, which was repeated every 5 s. Variable amplitude conditioning potentials (C) were followed by a test potential (T). Amplitudes of C (shown only to -10 mV) were extended to +10 mV. As C was increased, the amplitude of ICa,L evoked by T decreased. The fraction of maximum ICa,L evoked by T was plotted against the amplitude of C. Estimates of V0.5 and slope factor (k) were obtained using the Boltzmann function (inset), and are given in Table 3. Data are from six myocytes. R2for fits were not less than 0.998.
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Table 3. Parameters Derived from Fits of Steady-State Inactivation Curves 
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Table 3. Parameters Derived from Fits of Steady-State Inactivation Curves 
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Time of Recovery from Inactivation 
The time of recovery from inactivation, indexed by the time constant of recovery from inactivation, taurec, was prolonged by 10 and 100 micro Meter bupivacaine (Figure 6). Bupivacaine significantly increased taurecfrom the average control of 44.2 +/- 2.2 ms to 57.5 +/- 2.8 ms (10 micro Meter), and 93.5 +/- 5.5 ms (100 micro Meter). Wash did not restore taurecto the control level. Where P < 0.05 applied to 10 versus 100 micro Meter, and 100 micro Meter versus wash, it did not apply to wash versus 10 micro Meter.
Figure 6. Time of recovery from inactivation. Data points are the means of six myocytes. The schematic summarizes the stimulus protocol, which was repeated every 5 s. A test potential (T) followed a prepotential (P) by increasing intervals of time (Delta t). Standardized ICa,L (peak amplitude ratio of ICa,L T/P) was plotted against the interpotential interval (Delta t). The data were fit to a monoexponential function (inset), where t = abscissa values, to yield the solid line curves and estimates of the time constant of recovery from inactivation, taurec. R2for fits were not less than 0.997.
Figure 6. Time of recovery from inactivation. Data points are the means of six myocytes. The schematic summarizes the stimulus protocol, which was repeated every 5 s. A test potential (T) followed a prepotential (P) by increasing intervals of time (Delta t). Standardized ICa,L (peak amplitude ratio of ICa,L T/P) was plotted against the interpotential interval (Delta t). The data were fit to a monoexponential function (inset), where t = abscissa values, to yield the solid line curves and estimates of the time constant of recovery from inactivation, taurec. R2for fits were not less than 0.997.
Figure 6. Time of recovery from inactivation. Data points are the means of six myocytes. The schematic summarizes the stimulus protocol, which was repeated every 5 s. A test potential (T) followed a prepotential (P) by increasing intervals of time (Delta t). Standardized ICa,L (peak amplitude ratio of ICa,L T/P) was plotted against the interpotential interval (Delta t). The data were fit to a monoexponential function (inset), where t = abscissa values, to yield the solid line curves and estimates of the time constant of recovery from inactivation, taurec. R2for fits were not less than 0.997.
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Voltage-dependence of Inhibition 
To test whether the extent of bupivacaine inhibition was dependent on membrane voltage, we recorded peak amplitudes of ICa,L at holding potentials of -50 and -20 mV. The data are presented in Figure 7. At -50 mV, bupivacaine (30 micro Meter) inhibited ICa,L by 30.2 +/- 4.1%(Delta % of control). The change to -20 mV increased the inhibition by 55.8 +/- 6.0%(P < 0.008 for -50 vs. -20 mV data). Note that the amplitude of ICa,L was stable by 6–8 s. A monoexponential function was fit to the -20 mV data between 2–10 s with R2values between from 0.977 to 0.996. The average time constant equalled 1.40 +/- 0.08 s. The time constant indexed the rate of increased inhibition by bupivacaine established after the transition from -50 to -20 mV. In the absence of bupivacaine, the shift in holding potential, by itself, decreased the average amplitude of ICa,L from -1.05 +/- 0.05 nA (-50 mV) to -0.50 +/- 0.05 nA (-20 mV) by 20 s.
Figure 7. Voltage-dependence of bupivacaine inhibition (30 micro Meter). ICa,L was evoked by 200 ms command steps to +5 mV from a holding potential of -50 mV (0.5 Hz)(solid triangle). After the peak amplitude of ICa,L was stable, the holding potential was abruptly changed to -20 mV and 200 ms command steps to +5 mV were continued at 0.5 Hz (solid circles). ICa,L was recorded in the presence of 10 micro Meter TIX, which was sufficient to eliminate INaduring the step from -50 to +5 mV. Data are the average of three cardiomyocytes.
Figure 7. Voltage-dependence of bupivacaine inhibition (30 micro Meter). ICa,L was evoked by 200 ms command steps to +5 mV from a holding potential of -50 mV (0.5 Hz)(solid triangle). After the peak amplitude of ICa,L was stable, the holding potential was abruptly changed to -20 mV and 200 ms command steps to +5 mV were continued at 0.5 Hz (solid circles). ICa,L was recorded in the presence of 10 micro Meter TIX, which was sufficient to eliminate INaduring the step from -50 to +5 mV. Data are the average of three cardiomyocytes.
Figure 7. Voltage-dependence of bupivacaine inhibition (30 micro Meter). ICa,L was evoked by 200 ms command steps to +5 mV from a holding potential of -50 mV (0.5 Hz)(solid triangle). After the peak amplitude of ICa,L was stable, the holding potential was abruptly changed to -20 mV and 200 ms command steps to +5 mV were continued at 0.5 Hz (solid circles). ICa,L was recorded in the presence of 10 micro Meter TIX, which was sufficient to eliminate INaduring the step from -50 to +5 mV. Data are the average of three cardiomyocytes.
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The average extent of inhibition at -50 mV was greater but not statistically different (P = 0.15) than the inhibition at -40 mV by 30 micro Meter bupivacaine that was seen for the concentration-response plot of Figure 3.
Discussion 
In a concentration-dependent manner, bupivacaine tonically inhibited cardiac ICa,L. The average IC50was 112.0 micro Meter, as compared with an estimated 80.1 micro Meter for guinea pig ventricular myocytes at room temperature, [12 ] and 156 micro Meter for rat dorsal root ganglion (DRG) neurons in culture, also at room temperature. [8 ] The mid-range of our value suggests that temperature has little effect in altering the sensitivity of Ca channels to bupivacaine. The local anesthetic might be acting to increase the probability that L-type Ca channels reside in the inactivated state. The evidence for this comes from the negative-voltage shift in the steady-state inactivation curve (f [infinity]) and the increase in time of recovery from inactivation (taurec). The former suggests that fewer channels are available to open, whereas the latter indicates that it takes longer for the inactivated channels to recover before they become available to open. It is possible that tonic and use-dependent (phasic) inhibition may be occurring together in the normally functioning heart because bupivacaine may extend the time of recovery from inactivation to a point that the diastolic duration is too short to allow for a complete recovery from inactivation. However, bupivacaine is reported not to have a significant use-dependent effect. [12 ] It is also reported that the f [infinity] curve is not shifted by 50 micro Meter bupivacaine in ventricular cardiomyocytes of guinea pig, [12 ] a discrepancy which we are unable to explain.
In the present study, the Ca2+ chelator, EGTA, in the pipette dialyzing solution reduced but did not eliminate the effect of Ca-dependent inactivation on ICa,L. [15 ] The decreased influx of Ca2+ caused by bupivacaine might also have increased the inactivation time constants and masked an accelerating effect on the rates of inactivation. This idea is supported by the report that in frog atrial fibers, bupivacaine increased the inactivation time constant using a voltage clamp technique that did not buffer intracellular Ca2+. [11 ] The possibility of bupivacaine accelerating inactivation was tested by using Ba2+ as the charge carrier, thereby eliminating Ca-dependent inactivation as a factor. Under this condition, bupivacaine accelerated inactivation, although its contribution to decreasing the amplitude of ICa,L when Ca- and voltage-dependent inactivation are operative remains uncertain. The time to channel opening was slightly increased by bupivacaine, as suggested by the increase in TTP current. A bupivacaine-induced increase of TTP for ICa,L also is reported in cultured sensory neurons of rat [8 ] and in frog atrial fibers. [11 ]
If a voltage-dependence to the inhibition of ICa,L by bupivacaine could be shown, an explanation of the mechanism of inhibition would be consistent with the modulated receptor hypothesis. [16,17 ] Accordingly, bupivacaine would bind to channels differently depending on whether they were open, closed, or inactivated. If bupivacaine predisposes Ca channels to the inactivated state, it might be because it binds with greater affinity to the inactivated channels. For example, a greater proportion of Ca channels would be expected to be in the inactivated state at -20 mV (40% inactivated by Figure 5) than at a more hyperpolarized value, and our results showed that bupivacaine inhibited ICa,L more at -20 than -50 mV. Two studies that used ventricular cardiomyocytes of guinea pig report different results in this regard. One shows no difference in the extent of tonic inhibition at -40 and -70 mV holding potentials, [12 ] whereas the other demonstrates much more inhibition at -40 mV than at -90 mV. [9 ]
The characteristics of inhibition of ICa,L by bupivacaine have similarities and differences with that by dihydropyridine antagonists. Nisoldipine and nitrendipine, for example, prolong the time of recovery from inactivation of ICa,L. [18,19 ] Nevertheless, use-dependency of dihydropyridine block is much milder than that of diarylalkylamines (verapamil and D600). [20,21 ] The voltage-dependency of Ca channel block by dihydropyridines is marked compared with bupivacaine, and there is a rapid and complete washout of nitrendipine. [22 ] Nisoldipine causes a hyperpolarizing shift in the steady-state inactivation curve of ICa,L, [19 ] suggesting strongly that dihydropyridines interact with the inactivated state of the Ca channel. Finally, inhibition of cardiac ICa,L by bupivacaine is more potent in the presence of nifedipine, although it is uncertain which drug potentiates the other. [9 ]
Other local anesthetics inhibit ICa,L in neurons [8,23,24 ] and ventricular cardiomyocytes of chicks [25 ] and guinea pigs. [26 ] Tetracaine decreases the time constant of the slowly inactivating component of ICa,L in ventricular myocytes of guinea pig when Sr2+ or Ba2+ is the charge carrier. [26 ] In a comparison of the inhibitory effects of local anesthetics on ICa,L of rat DRG neurons, potencies are ranked as dibucaine > tetracaine > bupivacaine > > procaine = lidocaine; a sequence, the authors note, that closely matches the respective lipid solubilities. [8 ] In addition, other types of Ca channels have been examined for their sensitivity to local anesthetics. Tetracaine inhibits ICain N- and T-type Ca channels of rat DRG neurons. [8 ] Also, N-type ICain frog DRG neurons is inhibited by a derivative of bupivacaine, HS37, the S(-) being more potent than the R(+) isomer. [27 ]
The lowest concentration of bupivacaine HCl used in this study, 10 micro Meter (3.25 mg/ml), appears to be of dubious clinical significance because bupivacaine is reported to be 95.6%(0.4 mg/ml) to 87.7%(5.0 mg/ml) bound to human plasma protein. [28 ] However, 3.25 mg/ml may approach clinical relevance when free bupivacaine is increased by other drugs that displace it from plasma protein [29 ] or when bupivacaine potency is increased by combining it with calcium antagonists. [5,9 ] Also, fetuses may be more susceptible to bupivacaine because the percent of bound bupivacaine in fetal blood averages 66% at 1 mg/ml and 51% at 5 mg/ml. [30 ] Although the umbilical vein to maternal arterial concentration ratio is low for bupivacaine (0.25–0.31), [31 ] the ratio may increase during fetal acidemia, as it does with lidocaine by ion trapping. [32 ]
Interestingly, wash from 10 micro Meter bupivacaine was ineffective. A similar irreversibility of inhibition of ICa,L by 10 micro Meter bupivacaine is reported for guinea pig ventricular myocytes. [33 ] In addition, pretreatment with isoproterenol or dibutyryl cAMP appears to restore the reversibility of bupivacaine inhibition. [33 ] The prospect of a metabolic component being involved in bupivacaine-induced inhibition of ICa,L is further strengthened by the findings that bupivacaine reduces the production of cyclic AMP in human lymphocytes [34 ] and depletes high-energy phosphates in fibroblasts. [35 ] To explain the concentration versus wash effect, there may be two distinct mechanisms by which bupivacaine inhibits ICa,L. One is easily reversible and seen in higher concentrations, possibly the consequence of a low affinity binding to the Ca channel. The other is difficult to reverse and may be the result of either a higher affinity binding to the channel, or a less direct effect such as a reduced extent of channel phosphorylation. Clearly, more experimentation is needed to test these hypotheses. Because of its resistance to washout, the inhibition by 10 micro Meter bupivacaine might be of interest from a clinical perspective because bupivacaine cardiotoxicity is difficult to reverse. [1 ] In this sense, the in vitro result may be mimicking what is seen clinically.
In summary, bupivacaine tonically inhibits ICa,L in hamster ventricular cardiomyocytes. The changes in steady-state inactivation and time course of recovery from inactivation suggest that the local anesthetic predisposes L-type Ca channels to the inactivated state. Because wash from the low concentration is ineffective, a second mechanism of inhibition may be involved.
The authors thank Maida Liu-Barnett, M.T., for technical assistance and Dr. Patrick L. Ross and Dr. Erich Hirschberg for their helpful suggestions.
References 
References 
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Figure 1. Representative current traces with (A) Ca2+, and (B) Ba sup 2+ as charge carriers. Currents were evoked by 200 ms (A) and 300 ms (B) command steps to +5 mV (0.2 Hz stimulus frequency) from a holding potential of -40 mV. Dashed lines are fits of the data for determining the two time constants of inactivation by the Simplex least squares fitting method available in Clampfit (Table 1, Table 2). In (A), the two traces show the control and response to 100 micro Meter bupivacaine (2 min) from the same myocyte. Bupivacaine brought the holding current closer to 0 nA. In (B), the two traces of IBa,L show the control and response to 30 micro Meter bupivacaine (2 min) from the same myocyte.
Figure 1. Representative current traces with (A) Ca2+, and (B) Ba sup 2+ as charge carriers. Currents were evoked by 200 ms (A) and 300 ms (B) command steps to +5 mV (0.2 Hz stimulus frequency) from a holding potential of -40 mV. Dashed lines are fits of the data for determining the two time constants of inactivation by the Simplex least squares fitting method available in Clampfit (Table 1, Table 2). In (A), the two traces show the control and response to 100 micro Meter bupivacaine (2 min) from the same myocyte. Bupivacaine brought the holding current closer to 0 nA. In (B), the two traces of IBa,L show the control and response to 30 micro Meter bupivacaine (2 min) from the same myocyte.
Figure 1. Representative current traces with (A) Ca2+, and (B) Ba sup 2+ as charge carriers. Currents were evoked by 200 ms (A) and 300 ms (B) command steps to +5 mV (0.2 Hz stimulus frequency) from a holding potential of -40 mV. Dashed lines are fits of the data for determining the two time constants of inactivation by the Simplex least squares fitting method available in Clampfit (Table 1, Table 2). In (A), the two traces show the control and response to 100 micro Meter bupivacaine (2 min) from the same myocyte. Bupivacaine brought the holding current closer to 0 nA. In (B), the two traces of IBa,L show the control and response to 30 micro Meter bupivacaine (2 min) from the same myocyte.
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Figure 2. Inhibition of ICa,L by four concentrations of bupivacaine. Ordinates are fractions of peak ICa,L (i.e., ICa,l /ICa,L control) after correction for rundown, which did not exceed 0.012 nA/min. Currents were evoked as in Figure 1(A). Five minutes of control (open circles) was followed by 5 min of bupivacaine (filled circles, onset at upward arrow), and 5 min of wash (open circles, onset at downward arrow). N = 6 (10 micro Meter), 5 (30 micro Meter), 5 (100 micro Meter), and 6 (300 micro Meter) myocytes. SEM bars were not shown (see Figure 3for SEM bars).
Figure 2. Inhibition of ICa,L by four concentrations of bupivacaine. Ordinates are fractions of peak ICa,L (i.e., ICa,l /ICa,L control) after correction for rundown, which did not exceed 0.012 nA/min. Currents were evoked as in Figure 1(A). Five minutes of control (open circles) was followed by 5 min of bupivacaine (filled circles, onset at upward arrow), and 5 min of wash (open circles, onset at downward arrow). N = 6 (10 micro Meter), 5 (30 micro Meter), 5 (100 micro Meter), and 6 (300 micro Meter) myocytes. SEM bars were not shown (see Figure 3for SEM bars).
Figure 2. Inhibition of ICa,L by four concentrations of bupivacaine. Ordinates are fractions of peak ICa,L (i.e., ICa,l /ICa,L control) after correction for rundown, which did not exceed 0.012 nA/min. Currents were evoked as in Figure 1(A). Five minutes of control (open circles) was followed by 5 min of bupivacaine (filled circles, onset at upward arrow), and 5 min of wash (open circles, onset at downward arrow). N = 6 (10 micro Meter), 5 (30 micro Meter), 5 (100 micro Meter), and 6 (300 micro Meter) myocytes. SEM bars were not shown (see Figure 3for SEM bars).
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Figure 3. Inhibition of peak ICa,L versus bupivacaine concentration. Data are measured from the same cells used in Figure 2, and peak currents were measured at 5 min of drug application. Extents of inhibition (Delta % of control) are given. The data points were fit (solid lines) to a logistic function (inset) for estimating IC50, where y and x are ordinate and abscissa values, respectively. IC50= 112.0 +/- 9.1 micro Meter, and the slope factor n =-0.9881 +/- 0.008 micro Meter. The filled circles are larger than the SEM bars for 10 and 300 micro Meter. The size of SEM bars are the same as for the data point averages of Figure 2.
Figure 3. Inhibition of peak ICa,L versus bupivacaine concentration. Data are measured from the same cells used in Figure 2, and peak currents were measured at 5 min of drug application. Extents of inhibition (Delta % of control) are given. The data points were fit (solid lines) to a logistic function (inset) for estimating IC50, where y and x are ordinate and abscissa values, respectively. IC50= 112.0 +/- 9.1 micro Meter, and the slope factor n =-0.9881 +/- 0.008 micro Meter. The filled circles are larger than the SEM bars for 10 and 300 micro Meter. The size of SEM bars are the same as for the data point averages of Figure 2.
Figure 3. Inhibition of peak ICa,L versus bupivacaine concentration. Data are measured from the same cells used in Figure 2, and peak currents were measured at 5 min of drug application. Extents of inhibition (Delta % of control) are given. The data points were fit (solid lines) to a logistic function (inset) for estimating IC50, where y and x are ordinate and abscissa values, respectively. IC50= 112.0 +/- 9.1 micro Meter, and the slope factor n =-0.9881 +/- 0.008 micro Meter. The filled circles are larger than the SEM bars for 10 and 300 micro Meter. The size of SEM bars are the same as for the data point averages of Figure 2.
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Figure 4. Current versus voltage relationship. Averages of peak amplitudes of ICa,L were plotted against command voltages. Records were obtained at 2 to 2.5 min of exposure to each concentration, or 2 to 2.5 min of wash. Data are from five myocytes. ** denotes P < 0.05 for control versus 10 micro Meter, 100 micro Meter, and wash, respectively. * denotes P < 0.05 for control versus 100 micro Meter and wash, respectively (i.e., P > 0.05 for control vs. 10 micro Meter).
Figure 4. Current versus voltage relationship. Averages of peak amplitudes of ICa,L were plotted against command voltages. Records were obtained at 2 to 2.5 min of exposure to each concentration, or 2 to 2.5 min of wash. Data are from five myocytes. ** denotes P < 0.05 for control versus 10 micro Meter, 100 micro Meter, and wash, respectively. * denotes P < 0.05 for control versus 100 micro Meter and wash, respectively (i.e., P > 0.05 for control vs. 10 micro Meter).
Figure 4. Current versus voltage relationship. Averages of peak amplitudes of ICa,L were plotted against command voltages. Records were obtained at 2 to 2.5 min of exposure to each concentration, or 2 to 2.5 min of wash. Data are from five myocytes. ** denotes P < 0.05 for control versus 10 micro Meter, 100 micro Meter, and wash, respectively. * denotes P < 0.05 for control versus 100 micro Meter and wash, respectively (i.e., P > 0.05 for control vs. 10 micro Meter).
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Figure 5. Steady-state inactivation (f [infinity]). The schematic depicts the stimulus protocol, which was repeated every 5 s. Variable amplitude conditioning potentials (C) were followed by a test potential (T). Amplitudes of C (shown only to -10 mV) were extended to +10 mV. As C was increased, the amplitude of ICa,L evoked by T decreased. The fraction of maximum ICa,L evoked by T was plotted against the amplitude of C. Estimates of V0.5 and slope factor (k) were obtained using the Boltzmann function (inset), and are given in Table 3. Data are from six myocytes. R2for fits were not less than 0.998.
Figure 5. Steady-state inactivation (f [infinity]). The schematic depicts the stimulus protocol, which was repeated every 5 s. Variable amplitude conditioning potentials (C) were followed by a test potential (T). Amplitudes of C (shown only to -10 mV) were extended to +10 mV. As C was increased, the amplitude of ICa,L evoked by T decreased. The fraction of maximum ICa,L evoked by T was plotted against the amplitude of C. Estimates of V0.5 and slope factor (k) were obtained using the Boltzmann function (inset), and are given in Table 3. Data are from six myocytes. R2for fits were not less than 0.998.
Figure 5. Steady-state inactivation (f [infinity]). The schematic depicts the stimulus protocol, which was repeated every 5 s. Variable amplitude conditioning potentials (C) were followed by a test potential (T). Amplitudes of C (shown only to -10 mV) were extended to +10 mV. As C was increased, the amplitude of ICa,L evoked by T decreased. The fraction of maximum ICa,L evoked by T was plotted against the amplitude of C. Estimates of V0.5 and slope factor (k) were obtained using the Boltzmann function (inset), and are given in Table 3. Data are from six myocytes. R2for fits were not less than 0.998.
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Figure 6. Time of recovery from inactivation. Data points are the means of six myocytes. The schematic summarizes the stimulus protocol, which was repeated every 5 s. A test potential (T) followed a prepotential (P) by increasing intervals of time (Delta t). Standardized ICa,L (peak amplitude ratio of ICa,L T/P) was plotted against the interpotential interval (Delta t). The data were fit to a monoexponential function (inset), where t = abscissa values, to yield the solid line curves and estimates of the time constant of recovery from inactivation, taurec. R2for fits were not less than 0.997.
Figure 6. Time of recovery from inactivation. Data points are the means of six myocytes. The schematic summarizes the stimulus protocol, which was repeated every 5 s. A test potential (T) followed a prepotential (P) by increasing intervals of time (Delta t). Standardized ICa,L (peak amplitude ratio of ICa,L T/P) was plotted against the interpotential interval (Delta t). The data were fit to a monoexponential function (inset), where t = abscissa values, to yield the solid line curves and estimates of the time constant of recovery from inactivation, taurec. R2for fits were not less than 0.997.
Figure 6. Time of recovery from inactivation. Data points are the means of six myocytes. The schematic summarizes the stimulus protocol, which was repeated every 5 s. A test potential (T) followed a prepotential (P) by increasing intervals of time (Delta t). Standardized ICa,L (peak amplitude ratio of ICa,L T/P) was plotted against the interpotential interval (Delta t). The data were fit to a monoexponential function (inset), where t = abscissa values, to yield the solid line curves and estimates of the time constant of recovery from inactivation, taurec. R2for fits were not less than 0.997.
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Figure 7. Voltage-dependence of bupivacaine inhibition (30 micro Meter). ICa,L was evoked by 200 ms command steps to +5 mV from a holding potential of -50 mV (0.5 Hz)(solid triangle). After the peak amplitude of ICa,L was stable, the holding potential was abruptly changed to -20 mV and 200 ms command steps to +5 mV were continued at 0.5 Hz (solid circles). ICa,L was recorded in the presence of 10 micro Meter TIX, which was sufficient to eliminate INaduring the step from -50 to +5 mV. Data are the average of three cardiomyocytes.
Figure 7. Voltage-dependence of bupivacaine inhibition (30 micro Meter). ICa,L was evoked by 200 ms command steps to +5 mV from a holding potential of -50 mV (0.5 Hz)(solid triangle). After the peak amplitude of ICa,L was stable, the holding potential was abruptly changed to -20 mV and 200 ms command steps to +5 mV were continued at 0.5 Hz (solid circles). ICa,L was recorded in the presence of 10 micro Meter TIX, which was sufficient to eliminate INaduring the step from -50 to +5 mV. Data are the average of three cardiomyocytes.
Figure 7. Voltage-dependence of bupivacaine inhibition (30 micro Meter). ICa,L was evoked by 200 ms command steps to +5 mV from a holding potential of -50 mV (0.5 Hz)(solid triangle). After the peak amplitude of ICa,L was stable, the holding potential was abruptly changed to -20 mV and 200 ms command steps to +5 mV were continued at 0.5 Hz (solid circles). ICa,L was recorded in the presence of 10 micro Meter TIX, which was sufficient to eliminate INaduring the step from -50 to +5 mV. Data are the average of three cardiomyocytes.
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Table 1. Time Constants of Inactivation in Different Concentrations of Bupivacaine HCl 
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Table 1. Time Constants of Inactivation in Different Concentrations of Bupivacaine HCl 
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Table 2. Time Constants of Inactivation with Ba2+ as the Charge Carrier 
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Table 2. Time Constants of Inactivation with Ba2+ as the Charge Carrier 
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Table 3. Parameters Derived from Fits of Steady-State Inactivation Curves 
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Table 3. Parameters Derived from Fits of Steady-State Inactivation Curves 
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