Free
Meeting Abstracts  |   July 1998
Anesthetic-induced Alteration of Ca2+Homeostasis in Neural Cells  : A Temperature-sensitive Process That Is Enhanced by Blockade of Plasma Membrane Ca2+-ATPaseIsoforms
Author Notes
  • (J. J. Franks) Professor of Anesthesiology, Vanderbilt University.
  • (Wamil) Research Assistant Professor of Anesthesiology, Vanderbilt University.
  • (Janicki) Resident in Anesthesiology, Vanderbilt University.
  • (Horn) Assistant Professor of Anesthesiology, Vanderbilt University.
  • (W. T. Franks, Janson) Research Assistant, Vanderbilt University.
  • (Vanaman) Professor of Biochemistry, University of Kentucky.
  • (Brandt) Research Assistant Professor of Biochemistry, University of Kentucky.
Article Information
Meeting Abstracts   |   July 1998
Anesthetic-induced Alteration of Ca2+Homeostasis in Neural Cells  : A Temperature-sensitive Process That Is Enhanced by Blockade of Plasma Membrane Ca2+-ATPaseIsoforms
Anesthesiology 7 1998, Vol.89, 149-164. doi:
Anesthesiology 7 1998, Vol.89, 149-164. doi:
VARIOUS mechanisms of action for inhalation anesthetics have been postulated, and often the explanations center on alterations of central synaptic transmission, but proof remains elusive. It is, of course, a fundamental tenet of modern biology that small diffusible second messengers act within the cell to provide a link between stimulus and output, with cyclic nucleotides and their phosphorylation systems serving as classic examples. Second messenger functions for Ca2+are well established, [1] and the role of intracellular Ca2+in coupling neuronal excitation to the release of neurotransmitters into the synaptic space [2] makes anesthetic modulation of calcium homeostasis an attractive, although disputed, potential mechanism for anesthetic action.
Recently we observed that many inhalation anesthetics, in clinically relevant concentrations, inhibit plasma membrane Ca2+-adenosinetriphosphatase (ATPase; PMCA) ion pumping in brain synaptic membranes [3–5] and in isolated membranes of cultured cells of neural origin. [6] Parallel decreases or increases in requirements for anesthetics have been demonstrated in rat models with incidentally decreased [7–10] or increased [11] PMCA pumping activity. In addition, electrophysiologic and microfluorimetric studies of cultured mouse central neurons and dorsal root ganglion sensory cells revealed delayed repolarization and delayed restoration of intracellular calcium ([Ca2+](i)) to basal levels with exposure to halothane at 37 [degree sign]C, [12] changes that are consistent with anesthetic inhibition of PMCA.
In this study, we offer further evidence that anesthetics inhibit the plasma membrane calcium pump in cultured neural cells and thereby perturb Ca2+homeostasis. First, we report the effects of pharmacologically relevant concentrations of halothane, isoflurane, and sevoflurane on cytosolic Ca2+dynamics in cultured neurons and pheochromocytoma cells at physiologic and room temperatures. Second, we note halothane's effects on Ca2+dynamics in cells of neural origin with antisense blockade of specific PMCA isoform expression. Third, we report the effects of xenon, an inert element that serves as a clinically effective anesthetic, [13–14] on neuronal Ca2+homeostasis.
Materials and Methods
Mouse Neuron Preparation and Culture
Cerebral cortices were removed from mouse embryos [12] on gestational day 13 or 14. Tissue from 8–12 embryos was pooled, minced, and mechanically dissociated in a Ca2+-and Mg2+-freebalanced salt solution. Dissociated cells were suspended in plating medium consisting of equal parts (vol/vol) of Eagle's minimal essential medium (supplemented with 1.5 g/l sodium bicarbonate and 5.5 g/l glucose) and Hank's balanced salt solution to which the following was added: 5%(vol/vol) fetal calf serum, 10% horse serum, 10 ng/ml nerve growth factor, and 1 ml/l Mito Serum Extender (Life Technologies, Grand Island, NY). Aliquots of the suspension were placed in sterile collagen-coated, 35-mm, glass-bottom plastic dishes and kept at 37 [degree sign]C in an incubator with an atmosphere of 90% room air and 10% carbon dioxide to maintain pH near 7.4. [15] Growth of rapidly dividing non-neuronal cells in cortical cell cultures was suppressed by adding 5-fluoro-2'deoxyuridine and uridine (Sigma Chemical Co., St. Louis, MO; 50 [micro sign]g/ml each) to the culture medium. Subsequently, the medium was changed two or three times a week. Cultures were maintained for 4–20 weeks before the experiments.
Blockade of PMCA2 Expression in Cultured Mouse Embryonic Cortical Cells by Antisense PMCA2 Oligodeoxyribonucleotide
Cortical neurons were removed from mouse embryos on gestational day 13, before expression of PMCA2 occurs, [16] and cultured as just described here. Added to the medium was 10 [micro sign]g/ml of either a 21 base antisense PMCA2 oligodeoxyribonucleotide, 5'-CAT ATC ACC CAT GTT TGC TGA-3', or a 21 base oligodeoxyribonucleotide comprised of the same bases arranged in random order (scrambled bases), 5'-TCA GAC TTC GAT CCT TAA GCT-3', or neither. Media containing freshly thawed oligodeoxyribonucleotides were replaced daily. These oligonucleotides were prepared by the University of Kentucky Macromolecular Structure Analysis Facility using the solid-phase phosphoramidite method with incorporation of a phosphorothioate backbone. Each oligonucleotide preparation was purified chromatographically on either PD-10 gel filtration columns or C18 SepPak column cartridges (Waters, Marlboro, MA). (Neither preparation showed detectable cytotoxicity in pheochromocytoma cells at concentrations up to 50 [micro sign]g/ml.) After cortical embryonic neurons had been cultured in the presence of oligonucleotides for 8–14 days, by which time PMCA2 is ordinarily expressed, immunocytochemical analysis was done with PMCA2-specific antiserum and a fluorescent second antibody.
Rat Pheochromocytoma Cells with Blockade of PMCA1 Expression
The rat pheochromocytoma cell line, PC6, was used to prepare stably transfected cell lines in which all PMCA1 isoforms were blocked by constitutively expressed PMCA1-specific antisense RNA. The details of preparation and maintenance of these cell lines has been described. [17] Briefly, cDNA encoding the first 446 nucleotides of the human PMCA1 mRNA was inserted behind the RSV promoter in an orientation to produce antisense PMCA1 RNA or a short-sense control RNA and then transfected into PC6 cells. Cell lines stably expressing the antisense RNA with no detectable PMCA1 protein were selected and propagated in Dulbecco's minimum essential medium (Life Technologies) containing 10% horse serum and 5% fetal bovine serum. Cell lines were periodically tested for expression of the antisense RNA. It is noteworthy that cells with blocked PMCA1 production express 30% less total PMCA compared with controls, the remaining 70% coming from expression of PMCA2 and PMCA4 gene products. [17] 
Immunocytochemical Analysis
A modification of the method of Preiano et al. [18] was used. The medium was aspirated from culture plates, and cold fixative was added (3% paraformaldehyde [Sigma Chemical Co.] in phosphate-buffered saline [PBS]). After 30 min at room temperature, cells were washed twice and quickly frozen on dry ice. For staining, thawed cells were washed with PBS and then 0.1 M glycine (Sigma Chemical Co.) was added. After 20 min at room temperature and two additional washes, the cells were incubated for 1 h in a blocking solution containing 5%(vol/vol) goat serum (Sigma Chemical Co.), 0.1% bovine serum albumin (Sigma Chemical Co.), and 5%(vol/vol) glycerol (Sigma Chemical Co.) in PBS. After aspiration of the blocking solution, the appropriate dilution of the primary antibody serum in PBS (1:200–1:300) was added. The primary antibodies used in this procedure (and subsequent Western blot studies) were polyclonal antibodies raised in rabbits against recombinant human PMCA isoform fragments (first 80 amino acids from N terminal) and designated 1N, 2N, and 3N for human PMCA1, PMCA2, and PMCA3, respectively. [19] (Antiserum 4N, raised against PMCA4, was not used in this study because it does not react with rodent PMCA4.) After 1 h at room temperature, the cells were washed twice in PBS and the second, fluorescent antibody (1:200–1:300 of fluorescein isothiocyanate-conjugated polyclonal goat anti-rabbit antiserum; Sigma Chemical Co) in PBS was added. After 1 h, the cells were washed twice in PBS and placed on glass slides with Sigma mounting medium. Immunofluorescence was evaluated under an Olympus BHA microscope equipped with a fluorescence vertical illuminator (model BH-RFL, Melville, NY). Selected areas were photographed on ISO 400 Kodak color print film using a 1:400 objective. Exposure time for cell cultures was 45–60 s. The film images were scanned with a Kodak PhotoCD laser scanncer (Rochester, NY) with a resolution of 2048 x 3742 pixels, and image files were stored on CD-ROM discs.
Western Immunoblotting
Proteins were separated by electrophoresis on 7.5% polyacrylamide gel by the method of Laemmli. [20] Resolved proteins were transferred to 0.45 [micro sign]m Trans-Blot nitrocellulose filters (Bio-Rad, Rockville Center, NY) by electroblotting according to the method of Towbin et al. [21] These nitrocellulose filters were treated with blocking buffer (10 mM Tris-HCl, pH 7.8, 150 mM NaCl, and 0.1% Tween-20 with 1% bovine serum albumin) overnight at 4 [degree sign]C, washed twice at room temperature with wash buffer (blocking buffer with albumin reduced to 0.1%), and treated consecutively with 1N, 2N, and 3N antisera for 30 min. After three additional washes in wash buffer, the nitrocellulose filters were incubated for 30 min with goat anti-rabbit immunoglobulin G conjugated to horseradish peroxidase (Sigma Chemical Co.; final dilution, 1:2,000), followed by incubation for 2 or 3 min with a solution of diaminobenzidine-hydrogen peroxide as the chromogen.
Confocal Laser Scanning Microfluorimetry
Calcium imaging was done in the Vanderbilt University Cell Imaging Resource. A laser scanning confocal microscope (Zeiss LSM410, Seiles Instruments, St. Louis, MO), with diffraction-limited focusing of a laser beam, coupled with a special filter placed “confocal” to the sample, provides rejection of the out-of-focus background that is a thousand times better than a conventional microscope. The system is equipped with an argon - krypton laser for excitation. Neurons in dissociated monolayer cultures were loaded with fluo-3-AM (Molecular Probes, Eugene, OR) 40 min before the study. Culture dishes, 35 mm with glass bottoms for transmission of laser beams (MatTek, Ashland, MA), were placed in a microincubation system (Medical Systems Corp., Greenvale, NY) on the stage of the confocal microscope. The microincubation system allowed precise control of media temperature and was able to change the media temperature from 21 [degree sign]C to 37 [degree sign]C, and (in conjunction with superfusion) back to 21 [degree sign]C in <3 min. Dulbecco's modified PBS containing 1 mM CaCl2, 1 mM MgCl2, and 5.5 mM D-glucose served as control superfusate, delivered through Tygon (Norton Performance Plastics Corp., Akron, OH) tubing via a peristaltic pump [12] at a rate of 1 ml/min. Inhalation anesthetic solutions in the buffer in a 250-ml plastic bag (designed for intravenous infusions; Baxter, Deerfield, IL) were delivered in a similar way. Buffer volume in the dish was maintained at 1.5 ml by appropriate positioning of the outflow tubing. This buffer volume and superfusion rate, in conjunction with the microincubation system, allowed expeditious wash in and wash out of anesthetics in 3 or 4 min while the medium was maintained at the specified temperatures of 21 [degree sign]C or 37 [degree sign]C. For preparation of volatile anesthetic solutions, appropriate volumes were injected from a glass syringe into buffer in plastic bags from which the air had been expelled. The bags containing the anesthetic solutions were equilibrated for 2 h on a rocking device at 4 [degree sign]C and then allowed to warm to room temperature. Anesthetic concentrations were measured by high-performance liquid chromatography, gas chromatography, or both. [22–23] Concentrations were measured in samples taken from the bag (room temperature) and from the center of the microincubation dish (37 [degree sign]C). Losses from solutions in plastic bags could not be detected during a 4-h period after initial equilibration. Consistent, reproducible volatile anesthetic losses of about 50% were observed during passage of the solution through the delivery tubing and into the incubation dish. Initial volatile anesthetic solutions thus could be prepared so that the measured dish concentrations referred to throughout this report were equal to or just less than concentrations equivalent to 1 minimum alveolar concentration (MAC), except in experiments wherein significantly lower concentrations of anesthetic were needed. The MAC-equivalent concentrations at 37 [degree sign]C were 0.30 mM for halothane and 0.35 mM for isoflurane. [24] The MAC-equivalent for sevoflurane (MAC reported as 2.7% for young rats [25]) was estimated conservatively as 0.50 mM based on a series of experiments. Sevoflurane, 2.5% in air, was bubled through Dulbecco's modified phosphate buffer at 37 [degree sign]C for 1 h, and concentrations were compared with standard solutions. Buffer concentrations averaged 0.533 +/- 0.062 (SD) mM (n = 10).
Preparation of superfusion buffer containing xenon was done as follows. Dulbecco's modified buffer (100 ml) was placed in a 250-ml intravenous infusion bag. Air was expelled and replaced with 60 ml of a gas mixture composed of 20% oxygen and xenon-nitrogen in ratios providing 20%, 40%, 60%, and 80% xenon. (These gas volumes would appear to be adequate for solution preparation, because the relative solubilities of nitrogen, oxygen, and xenon are approximately 18, 32, and 119 ml per liter of water at room temperature and ambient pressure. [26]) Thus all xenon concentrations used in these experiments were less than the MAC, which was reported as 95% in mice. [27] Bags containing the buffer and gas mixtures were placed on a rocking device, maintained at 4 [degree sign]C overnight, and used the next day for microfluorimetric analysis. Bags were warmed to 37 [degree sign]C before use to avoid the possibility of xenon effervescence when the superfusate was transferred to the incubation dish. Taking into account the vapor pressure of water, xenon concentrations in the superfusate would have been equivalent to 0.2, 0.4, 0.6, and 0.8 MAC if there was no loss in transit. We could not measure dish concentrations of xenon, but the observed effects of xenon superfusates on neuronal [Ca2+]isuggest that loss rates during delivery to the cells were smaller than those seen with volatile anesthetics.
Influx of Ca2+into cells was induced by application of a ligand via a patch-clamp pipette within 3 s of starting the laser beams. Fluorescence intensity in sequential images (1 or 2 per second) of selected microscopic fields was digitally recorded with a resolution of 256 x 256 pixels and stored on zip-drive cartridges. (Control experiments done at 21 [degree sign]C and 37 [degree sign]C without ligand application showed no artifactual bleaching with this procedure. Nor was there any significant change in Ca2+-reducedfluorescence in the absence of ligand and anesthetic.) Microscopic fields in three or more culture dishes were selected so that imaged cells lay within 100 [micro sign]m of the tip of the patch-clamp pipette. The time-courses of Ca2+-associatedfluorescence in magnified images of randomly selected individual cells in a field were subsequently analyzed and converted to scalar values of fluorescence with the LSM410-associated software. Measurement of fluorescence in a fixed field containing a single cell allowed accurate measurement of relative changes in [Ca2+]iconsequent to ligand stimulation and anesthetic exposure.
(Figure 1) illustrates this process of analysis. Sixty-four sequential images of a microscopic field of cells were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA). Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated mouse embryonic cortical neuron and the upper from that same neuron after a 10-min anesthetic exposure. Each curve is derived from computer conversion of fluorescence intensity of 64 sequential images to arbitrary units of fluorescence. Selected images are shown in the Figure toillustrate how points on the graph were obtained. The abscissa shows time in seconds, and the ordinate indicates fluorescence in arbitrary units. As is clear in both sequences of images, Ca2+-associatedfluorescence increased after NMDA application, and there is subsequent clearance of calcium back to prestimulation levels. This analog output derived from digitized images of Ca2+-associatedfluorescence provides three parameters of interest: baseline fluorescence, observed before each application of NMDA or other ligand; peak fluorescence achieved by ligand-stimulated calcium influx; and Ca2+clearance time (i.e., the time required for return of Ca2+-associatedfluorescence to the prestimulus level). For clarity in illustrating anesthetic effects on [Ca2+]ifluorescence shown in summary figures in the Results section has been normalized:
Figure 1. Analysis of the time course of Ca2+-relatedfluorescence in a mouse embryonic cortical neuron. Sequential digitized images of a microscopic field of cells previously loaded with Flu3-AM (a calcium-sensitive dye) were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA; 10-4M) and stored on a zip drive. Subsequently, single cells in the field were chosen at random and magnified for analysis. Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated (control) neuron and the upper from the same cell after a 10-min superfusion with an inhalation anesthetic solution (0.3 mM halothane). Each curve is derived from a computer conversion of fluorescence intensity of 64 sequential images to scalar values of fluorescence. Typical images are shown in the Figure toillustrate how points on the graph are obtained. The abscissa shows time in seconds (s), and the ordinate indicates arbitrary units (au) of fluorescence provided by the LSM410-associated software. As is clear in both sequences of images, Ca2+-associatedfluorescence is increased after NMDA application, and calcium clearance subsequently returns to prestimulation levels.
Figure 1. Analysis of the time course of Ca2+-relatedfluorescence in a mouse embryonic cortical neuron. Sequential digitized images of a microscopic field of cells previously loaded with Flu3-AM (a calcium-sensitive dye) were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA; 10-4M) and stored on a zip drive. Subsequently, single cells in the field were chosen at random and magnified for analysis. Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated (control) neuron and the upper from the same cell after a 10-min superfusion with an inhalation anesthetic solution (0.3 mM halothane). Each curve is derived from a computer conversion of fluorescence intensity of 64 sequential images to scalar values of fluorescence. Typical images are shown in the Figure toillustrate how points on the graph are obtained. The abscissa shows time in seconds (s), and the ordinate indicates arbitrary units (au) of fluorescence provided by the LSM410-associated software. As is clear in both sequences of images, Ca2+-associatedfluorescence is increased after NMDA application, and calcium clearance subsequently returns to prestimulation levels.
Figure 1. Analysis of the time course of Ca2+-relatedfluorescence in a mouse embryonic cortical neuron. Sequential digitized images of a microscopic field of cells previously loaded with Flu3-AM (a calcium-sensitive dye) were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA; 10-4M) and stored on a zip drive. Subsequently, single cells in the field were chosen at random and magnified for analysis. Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated (control) neuron and the upper from the same cell after a 10-min superfusion with an inhalation anesthetic solution (0.3 mM halothane). Each curve is derived from a computer conversion of fluorescence intensity of 64 sequential images to scalar values of fluorescence. Typical images are shown in the Figure toillustrate how points on the graph are obtained. The abscissa shows time in seconds (s), and the ordinate indicates arbitrary units (au) of fluorescence provided by the LSM410-associated software. As is clear in both sequences of images, Ca2+-associatedfluorescence is increased after NMDA application, and calcium clearance subsequently returns to prestimulation levels.
×
relative fluorescence =(fluorescence in arbitrary units at time t)/(mean initial baseline fluorescence in arbitrary units at time 0).
Thus initial, control baseline fluorescence is normalized to unity, and subsequent measures of fluorescence, whether baseline or peak, are given as fractional increases above control baseline.
All chemicals and reagents used were of the highest grade available and supplied by Sigma Chemical Company unless otherwise specified.
Statistical Analysis
Data were examined by (1) multifactorial analysis of variance and the Student-Newman-Keuls procedure for multiple comparison of means and (2) the Kruskal-Wallis nonparametric procedure, with box-and-whiskers plots to determine which medians differed significantly.
Results
Temperature-specific Anesthetic Effects on Neuronal Calcium Dynamics
(Figure 2) illustrates the effect of a clinically relevant concentration of halothane at 37 [degree sign]C on the Ca2+homeostatic response of a cultured embryonic mouse cortical neuron when Ca2+influx was induced by application of NMDA. Figure 3summarizes these experiments and gives results obtained when cortical neurons were exposed to halothane at 21 [degree sign]C. As indicated in both figures and reported previously, [12] halothane, at concentrations at the lower end of the usual pharmacologic dose, prolonged the clearance time of intracellular Ca2+threefold compared with pre-exposure control responses (Figure 3A). In addition, these experiments showed that exposure to halothane for 10 min produced a 2.5-fold upward shift of baseline Ca2+-associatedfluorescence before a second application of NMDA and an increase in the peak fluorescence attained after NMDA application (Figure 3B). These parameters returned to normal after a 10-min halothane washout. In contrast, no significant changes in clearance time or in baseline and peak [Ca2+]ioccurred with halothane exposure at 21 [degree sign]C. Figure 4shows the effects of isoflurane and sevoflurane on Ca2+dynamics in cortical neurons. As with halothane, prolongation of elapsed time to baseline (Figure 4A) and elevation of baseline and peak fluorescence (Figure 4B) occurred with exposure of neurons at 37 [degree sign]C. No significant isoflurane (n = 10) or sevoflurane (n = 8) effects on Ca2+dynamics were seen in cells maintained at 21 [degree sign]C.
Figure 2. Halothane alteration of Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response of a representative cultured mouse embryonic cortical neuron before, during, and after exposure to 0.30 mM halothane. The abscissa shows seconds (s), and the ordinate shows Ca2+-relatedfluorescence in arbitrary units (au). NMDA (10-4M) was applied for 3 s, indicated by horizontal bars, after 10 min of superfusion with Dulbecco's modified buffer (see text), buffer with halothane, or buffer alone. Cells were maintained at 37 [degree sign]C.
Figure 2. Halothane alteration of Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response of a representative cultured mouse embryonic cortical neuron before, during, and after exposure to 0.30 mM halothane. The abscissa shows seconds (s), and the ordinate shows Ca2+-relatedfluorescence in arbitrary units (au). NMDA (10-4M) was applied for 3 s, indicated by horizontal bars, after 10 min of superfusion with Dulbecco's modified buffer (see text), buffer with halothane, or buffer alone. Cells were maintained at 37 [degree sign]C.
Figure 2. Halothane alteration of Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response of a representative cultured mouse embryonic cortical neuron before, during, and after exposure to 0.30 mM halothane. The abscissa shows seconds (s), and the ordinate shows Ca2+-relatedfluorescence in arbitrary units (au). NMDA (10-4M) was applied for 3 s, indicated by horizontal bars, after 10 min of superfusion with Dulbecco's modified buffer (see text), buffer with halothane, or buffer alone. Cells were maintained at 37 [degree sign]C.
×
Figure 3. Summary of the temperature-dependent effects of halothane on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cortical neurons. Each cell was stimulated by N-methyl-D-aspartate (NMDA) after sequential superfusion for 10 min with buffer (control 1), buffer with 0.30 mM halothane, and buffer (control 2). Experimental conditions were as described in Figure 2. The ordinates give time and relative Ca2+-associated fluorescence, the latter normalized so that initial, prehalothane baseline is unity. Subsequent pre-NMDA baseline (BL) and post-NMDA peak (PK) fluorescence levels are shown as fractional increases above initial baseline. Columns show mean values with 99% confidence limits. Asterisks denote significant prolongation of Ca2+clearance time and elevation of baseline and peak [Ca2+]iin cells exposed to halothane at 37 [degree sign]C (P < 0.01, n = 22). No halothane effects were seen at 21 [degree sign]C (n = 8).
Figure 3. Summary of the temperature-dependent effects of halothane on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cortical neurons. Each cell was stimulated by N-methyl-D-aspartate (NMDA) after sequential superfusion for 10 min with buffer (control 1), buffer with 0.30 mM halothane, and buffer (control 2). Experimental conditions were as described in Figure 2. The ordinates give time and relative Ca2+-associated fluorescence, the latter normalized so that initial, prehalothane baseline is unity. Subsequent pre-NMDA baseline (BL) and post-NMDA peak (PK) fluorescence levels are shown as fractional increases above initial baseline. Columns show mean values with 99% confidence limits. Asterisks denote significant prolongation of Ca2+clearance time and elevation of baseline and peak [Ca2+]iin cells exposed to halothane at 37 [degree sign]C (P < 0.01, n = 22). No halothane effects were seen at 21 [degree sign]C (n = 8).
Figure 3. Summary of the temperature-dependent effects of halothane on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cortical neurons. Each cell was stimulated by N-methyl-D-aspartate (NMDA) after sequential superfusion for 10 min with buffer (control 1), buffer with 0.30 mM halothane, and buffer (control 2). Experimental conditions were as described in Figure 2. The ordinates give time and relative Ca2+-associated fluorescence, the latter normalized so that initial, prehalothane baseline is unity. Subsequent pre-NMDA baseline (BL) and post-NMDA peak (PK) fluorescence levels are shown as fractional increases above initial baseline. Columns show mean values with 99% confidence limits. Asterisks denote significant prolongation of Ca2+clearance time and elevation of baseline and peak [Ca2+]iin cells exposed to halothane at 37 [degree sign]C (P < 0.01, n = 22). No halothane effects were seen at 21 [degree sign]C (n = 8).
×
Figure 4. Ca2+clearance (A) and baseline and peak relative [Ca2+]i(B) in cortical neurons exposed at 37 [degree sign]C to isoflurane or sevoflurane. N-methyl-D-aspartate was applied after sequential superfusion for 10 min or more with buffer (control 1), buffer with either 0.35 mM isoflurane or 0.50 mM sevoflurane, and buffer (control 2). Both agents prolonged Ca2+clearance and elevated baseline and peak [Ca2+]i(P < 0.01, n = 10 for isoflurane and n = 8 for sevoflurane).
Figure 4. Ca2+clearance (A) and baseline and peak relative [Ca2+]i(B) in cortical neurons exposed at 37 [degree sign]C to isoflurane or sevoflurane. N-methyl-D-aspartate was applied after sequential superfusion for 10 min or more with buffer (control 1), buffer with either 0.35 mM isoflurane or 0.50 mM sevoflurane, and buffer (control 2). Both agents prolonged Ca2+clearance and elevated baseline and peak [Ca2+]i(P < 0.01, n = 10 for isoflurane and n = 8 for sevoflurane).
Figure 4. Ca2+clearance (A) and baseline and peak relative [Ca2+]i(B) in cortical neurons exposed at 37 [degree sign]C to isoflurane or sevoflurane. N-methyl-D-aspartate was applied after sequential superfusion for 10 min or more with buffer (control 1), buffer with either 0.35 mM isoflurane or 0.50 mM sevoflurane, and buffer (control 2). Both agents prolonged Ca2+clearance and elevated baseline and peak [Ca2+]i(P < 0.01, n = 10 for isoflurane and n = 8 for sevoflurane).
×
Blockade of PMCA2 Production in Cultured Mouse Embryonic Cortical Cells by Antisense PMCA2 Oligodeoxyribonucleotide
Cortical neurons in culture were treated with either an antisense PMCA2 oligodeoxyribonucleotide, an oligodeoxyribonucleotide with the bases arranged in random order (scrambled bases), or neither one. Immunocytochemical analyses were done with PMCA2-specific antiserum and a fluorescent second antibody. Fluorescence of scrambled base-treated and -untreated (not shown) cells indicated the presence of PMCA2, whereas only background fluorescence was observed in antisense-treated neurons (Figure 5).
Figure 5. Immunofluorescent staining of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Cells were immunostained with isoform-specific rabbit antibody, which reacts only with PMCA2. (Left) Control neurons showing PMCA2 specific immunostaining. (Right) Reduced immunostaining intensity resulting from deficiency in PMCA2 (x1,200).
Figure 5. Immunofluorescent staining of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Cells were immunostained with isoform-specific rabbit antibody, which reacts only with PMCA2. (Left) Control neurons showing PMCA2 specific immunostaining. (Right) Reduced immunostaining intensity resulting from deficiency in PMCA2 (x1,200).
Figure 5. Immunofluorescent staining of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Cells were immunostained with isoform-specific rabbit antibody, which reacts only with PMCA2. (Left) Control neurons showing PMCA2 specific immunostaining. (Right) Reduced immunostaining intensity resulting from deficiency in PMCA2 (x1,200).
×
Increased Halothane Sensitivity of Cultured Mouse Embryonic Cortical Cells with Blocked PMCA2 Production
When examined by confocal microfluorimetry, antisense-treated neurons manifested significantly increased sensitivity to halothane, compared with wild-type and scrambled base-treated cells. Figure 6shows the results from a typical experiment. Each row shows the Ca2+-dependentfluorescence response in cells of each type when subjected to different treatments. The recordings in the first column indicate that application of NMDA resulted in a brisk influx of Ca2+in all cell types. Low-dose halothane (<or= to 0.15 mM, recordings in the second column) had little effect on Ca2+dynamics in wild-type and scrambled base-treated cells, in contrast to antisense-treated cells. Moderate doses of halothane (<or= to 0.30 mM, recordings in the fourth column) affected all three cell types. Figure 7summarizes the results in these cells. On the average, Ca2+clearance time in antisense-treated neurons was doubled by exposure to low-dose halothane, whereas no effect was seen in wild-type and scrambled base-treated cells (Figure 7A). Moderate doses of halothane prolonged Ca (2+) clearance in all three types, especially in antisense cells. A 10-min exposure to 0.15 mM halothane also shifted pre-NMDA baseline fluorescence upward by an average of 2.5 times and significantly increased peak fluorescence after NMDA (Figure 7B). This lower concentration had no effect on these parameters in wild-type and scrambled base-treated cells. In contrast, 0.30 mM halothane prolonged clearance time and increased baseline and peak fluorescence in all three cell types. No significant halothane effects on Ca2+dynamics were observed in antisense-treated cells maintained at 21 [degree sign]C (n = 10).
Figure 6. Increased anesthetic sensitivity of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Experimental conditions were the same as described in Figure 2. Shown are microfluorimetric recordings of the Ca2+response in wild-type (top row), antisense oligodeoxyribonucleotide-treated (middle row), and scrambled base-treated (bottom row) neurons. N-methyl-D-aspartate was applied after 10 min of sequential superfusion with buffer, buffer with 0.15 mM halothane, buffer, buffer with 0.30 mM halothane, and again with buffer.
Figure 6. Increased anesthetic sensitivity of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Experimental conditions were the same as described in Figure 2. Shown are microfluorimetric recordings of the Ca2+response in wild-type (top row), antisense oligodeoxyribonucleotide-treated (middle row), and scrambled base-treated (bottom row) neurons. N-methyl-D-aspartate was applied after 10 min of sequential superfusion with buffer, buffer with 0.15 mM halothane, buffer, buffer with 0.30 mM halothane, and again with buffer.
Figure 6. Increased anesthetic sensitivity of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Experimental conditions were the same as described in Figure 2. Shown are microfluorimetric recordings of the Ca2+response in wild-type (top row), antisense oligodeoxyribonucleotide-treated (middle row), and scrambled base-treated (bottom row) neurons. N-methyl-D-aspartate was applied after 10 min of sequential superfusion with buffer, buffer with 0.15 mM halothane, buffer, buffer with 0.30 mM halothane, and again with buffer.
×
Figure 7. Summary of effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-treated neurons. These same Ca2+parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).
Figure 7. Summary of effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-treated neurons. These same Ca2+parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).
Figure 7. Summary of effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-treated neurons. These same Ca2+parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).
×
PMCA1 Isoform Expression in Pheochromocytoma Cells Stably Transfected with Sense- and Antisense-producing Constructs
(Figure 8) shows the results of Western immunoblotting with PMCA isoform-specific antisera of (1) purified human erythrocytic PMCA (isoforms 1 and 4; provided by Dr. John Penniston), (2) rat brain synaptic plasma membrane, (3) crude plasma membrane fractions of sense-transfected (RSV14) pheochromocytoma cells, (4) antisense-transfected (RSV9) pheochromocytoma cells, and (5) wild-type (PC6) pheochromocytoma cells. Figure 8indicates that isoform 1 was present in all samples except antisense-transfected pheochromocytoma cells, whereas isoform 2 was present in all samples except erythrocytic PMCA, as expected. Isoform 3, confined in the brain to the choroid plexus, was not found in any of these samples. As noted before, antiserum against rodent PMCA4 was not available. Immunocytochemical analysis (not shown) confirmed the absence of PMCA1 in antisense-transfected cells.
Figure 8. Western immunoblot analysis of PMCA isoforms in plasma membranes of transfected pheochromocytoma cells. Column 1 shows purified erythrocytic PMCA1 and PMCA4, and column 2 shows rat cortical synaptic plasma membranes, for reference. Column 3 shows membrane isolates from cells transfected with PMCA1 sense cDNA (RSV14), column 4 shows isolates from cells transfected with PMCA1 antisense cDNA (RSV9), and column 5 shows isolates from wild-type (PC6). Rabbit antisera 1N, 2N, and 3N react with PMCA isoforms 1, 2, and 3. Marker protein positions are indicated along the left margin.
Figure 8. Western immunoblot analysis of PMCA isoforms in plasma membranes of transfected pheochromocytoma cells. Column 1 shows purified erythrocytic PMCA1 and PMCA4, and column 2 shows rat cortical synaptic plasma membranes, for reference. Column 3 shows membrane isolates from cells transfected with PMCA1 sense cDNA (RSV14), column 4 shows isolates from cells transfected with PMCA1 antisense cDNA (RSV9), and column 5 shows isolates from wild-type (PC6). Rabbit antisera 1N, 2N, and 3N react with PMCA isoforms 1, 2, and 3. Marker protein positions are indicated along the left margin.
Figure 8. Western immunoblot analysis of PMCA isoforms in plasma membranes of transfected pheochromocytoma cells. Column 1 shows purified erythrocytic PMCA1 and PMCA4, and column 2 shows rat cortical synaptic plasma membranes, for reference. Column 3 shows membrane isolates from cells transfected with PMCA1 sense cDNA (RSV14), column 4 shows isolates from cells transfected with PMCA1 antisense cDNA (RSV9), and column 5 shows isolates from wild-type (PC6). Rabbit antisera 1N, 2N, and 3N react with PMCA isoforms 1, 2, and 3. Marker protein positions are indicated along the left margin.
×
Increased Halothane Sensitivity of Transfected Pheochromocytoma Cells with Blocked Production of PMCA1
Using confocal laser scanning microfluorimetric analysis, we examined the effect of halothane on cytosolic Ca2+dynamics in antisense-transfected cells compared with wild-type and sense-transfected controls. Ca2+influx into the cytoplasm was induced by bradykinin. [17] Because bradykinin mobilizes internal Ca2+, applications were separated by at least 20 min to allow restoration of internal Ca2+stores. Pheochromocytoma cells transfected with PMCA1 antisense proved to be even more sensitive to halothane than antisense PMCA2 oligodeoxyribonucleotide-treated cortical neurons, as shown in Figure 9. Very low dose halothane (<or= to 0.03 mM) increased Ca2+clearance time nearly threefold, compared with unexposed controls, but had no effect on Ca (2+) clearance in wild-type and sense-transfected cells (Figure 9A). A moderate dose halothane (<or= to 0.3 mM) prolonged Ca2+clearance in all three cell types. In addition, very low dose halothane substantially increased resting baseline and postligand peak Ca2+in antisense-transfected cells, in contrast to wild-type and sense-transfected cells (Figure 9B). A moderate dose of halothane increased pre- and poststimulation [Ca2+]iin all three pheochromocytoma cell types.
Figure 9. Effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca2+parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).
Figure 9. Effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca2+parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).
Figure 9. Effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca2+parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).
×
Xenon Effects on Neuronal Calcium Dynamics
Exposure to xenon produced striking alterations in Ca2+homeostasis in cultured cortical neurons. Figure 10shows these effects on a cell exposed successively to xenon solutions that were, at the maximum, equivalent to 0.2, 0.4, 0.6, and 0.8 MAC, and Figure 11summarizes the results from 10 such studies. Significant differences in Ca2+responses were seen with exposure to 0.6 and 0.8 MAC. Ca2+clearance time was prolonged more than two and five times, respectively, compared with the prexenon treatment period (Figure 11A). A small but significant prolongation of clearance time occurred with 0.40 MAC xenon. Pre-NMDA baseline [Ca2+]iwas increased 1.7 times with 0.60 and more than two times with 0.80 MAC xenon, compared with the prexenon control response, whereas post-NMDA peak [Ca2+]iincreased to 1.5 and 1.8 times that of control (Figure 11B).
Figure 10. Xenon effects on Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response in a representative cultured mouse embryonic cortical neuron exposed to increasing concentrations of xenon (all less than the minimum alveolar concentration [MAC]). NMDA was applied after 10 min of sequential superfusion with buffers equilibrated with dry gas mixtures of 20% oxygen and xenon-nitrogen in ratios providing xenon partial pressures ranging from 0–80% of 1 atm. Calculated maximum MAC-equivalent values of xenon solutions were 0.20, 0.40, 0.60, and 0.80, as described in the text.
Figure 10. Xenon effects on Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response in a representative cultured mouse embryonic cortical neuron exposed to increasing concentrations of xenon (all less than the minimum alveolar concentration [MAC]). NMDA was applied after 10 min of sequential superfusion with buffers equilibrated with dry gas mixtures of 20% oxygen and xenon-nitrogen in ratios providing xenon partial pressures ranging from 0–80% of 1 atm. Calculated maximum MAC-equivalent values of xenon solutions were 0.20, 0.40, 0.60, and 0.80, as described in the text.
Figure 10. Xenon effects on Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response in a representative cultured mouse embryonic cortical neuron exposed to increasing concentrations of xenon (all less than the minimum alveolar concentration [MAC]). NMDA was applied after 10 min of sequential superfusion with buffers equilibrated with dry gas mixtures of 20% oxygen and xenon-nitrogen in ratios providing xenon partial pressures ranging from 0–80% of 1 atm. Calculated maximum MAC-equivalent values of xenon solutions were 0.20, 0.40, 0.60, and 0.80, as described in the text.
×
Figure 11. Summary of effects of different concentrations of xenon on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cultured cortical neurons. Exposure conditions and procedures are described in Figure 10. Columns in part A give 95% confidence limits, whereas those in part B give 99% confidence limits. Differences among responses to varying xenon concentrations are defined by line segments adjacent to each response parameter. Statistical significance, based on multifactor analysis of variance, is indicated when segments are discontinuous and do not overlap (P < 0.05 for panel A and P < 0.01 for panel B, n = 10). Nonparametric testing (Kruskal-Wallis) confirmed the differences observed in Ca+-associatedfluorescence (panel B) and indicated that median clearance times in cells exposed to xenon minimum alveolar concentration-equivalent values of 0.40, 0.60, and 0.80 differed significantly from each other and from 0 and 0.20 (P < 0.01). Thus a clear pattern of increased perturbation of Ca2+dynamics with increasing xenon concentrations was observed.
Figure 11. Summary of effects of different concentrations of xenon on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cultured cortical neurons. Exposure conditions and procedures are described in Figure 10. Columns in part A give 95% confidence limits, whereas those in part B give 99% confidence limits. Differences among responses to varying xenon concentrations are defined by line segments adjacent to each response parameter. Statistical significance, based on multifactor analysis of variance, is indicated when segments are discontinuous and do not overlap (P < 0.05 for panel A and P < 0.01 for panel B, n = 10). Nonparametric testing (Kruskal-Wallis) confirmed the differences observed in Ca+-associatedfluorescence (panel B) and indicated that median clearance times in cells exposed to xenon minimum alveolar concentration-equivalent values of 0.40, 0.60, and 0.80 differed significantly from each other and from 0 and 0.20 (P < 0.01). Thus a clear pattern of increased perturbation of Ca2+dynamics with increasing xenon concentrations was observed.
Figure 11. Summary of effects of different concentrations of xenon on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cultured cortical neurons. Exposure conditions and procedures are described in Figure 10. Columns in part A give 95% confidence limits, whereas those in part B give 99% confidence limits. Differences among responses to varying xenon concentrations are defined by line segments adjacent to each response parameter. Statistical significance, based on multifactor analysis of variance, is indicated when segments are discontinuous and do not overlap (P < 0.05 for panel A and P < 0.01 for panel B, n = 10). Nonparametric testing (Kruskal-Wallis) confirmed the differences observed in Ca+-associatedfluorescence (panel B) and indicated that median clearance times in cells exposed to xenon minimum alveolar concentration-equivalent values of 0.40, 0.60, and 0.80 differed significantly from each other and from 0 and 0.20 (P < 0.01). Thus a clear pattern of increased perturbation of Ca2+dynamics with increasing xenon concentrations was observed.
×
Discussion
Regulation of cytosolic Ca2+concentration ([Ca2+]i) is critical for maintaining a balance between rapid Ca2+signaling [28] and Ca2+-dependenttoxicity [29] and must be done against a 10,000 to 1 gradient of extracellular to intracellular [Ca2+]. Ca2+channels in the plasma membrane and intracellular release channels in the endoplasmic reticulum can increase [Ca2+]irapidly with appropriate stimulation. Three principal systems serve to reduce or maintain [Ca2+](i) at low levels: a plasma membrane Na+/Ca2+exchanger, a plasma membrane Ca2+-ATPasepump (PMCA), and a smooth endoplasmic reticulum Ca (2+-ATPase) pump. Mitochondrial uptake and release functions are thought to operate principally at high [Ca2+]i. [30] 
The development in the last decade of powerful methods for recording changes in [Ca2+]iin single cells, based on electrophysiologic and Ca2+-specificmicrofluorimetric techniques, has led to a substantial advance in knowledge of the complexities of neuronal [Ca (2+)]iregulation and signaling. Ca2+influx via channels and release from intracellular stores have been studied extensively. [31] So also, although to a lesser extent, have been the modulation of processes that remove Ca2+from neuronal cytosol [32] and their relative importance in neuronal function. The consensus has been that the high capacity and low affinity of the Na+/Ca2+exchanger relegates its role to removal of Ca2+when concentrations are 1 [micro sign]M or more, whereas high-affinity PMCA serves as a precise regulator that maintains [Ca2+](i) at low resting levels. [33] 
Several interesting studies have shed light on just how important PMCA is in removing Ca2+loads in neurons. Benham et al., [34] using patch-clamp and microfluorimetric techniques in cultured rat dorsal root ganglion cells, showed that whereas Na+/Ca2+exchange played only a small part in removing physiologic Ca2+loads (approximately 500 nM) and no apparent part in maintaining resting [Ca2+]i, inactivation of PMCA with orthovanadate or high pH dramatically showed Ca2+removal and significantly increased baseline [Ca2+]i. They concluded that PMCA is critical for the removal of Ca2+loads of similar amplitude to those generated by the firing of action potentials. Using similar techniques, Bleakman et al. [35] showed that mitochondria, endoplasmic reticulum stores, and the Na+/Ca2+exchanger have little effect on short-term clearance of modest Ca2+loads in cultured rat septal neurons. More recently, Werth et al., [36] in an extensive study, evaluated Ca2+efflux systems in rat dorsal root ganglion cells and confirmed that PMCA-mediated Ca2+extrusion is the primary process responsible for recovery to basal [Ca2+]iafter stimulation. They showed that sequestration of Ca2+in intracellular stores or extrusion of Ca2+from the cell via the Na+/Ca2+exchanger contributed minimally to recovery of [Ca2+]ito baseline. On the other hand, treatment of neurons with C28R2, a synthetic peptide representing the autoinhibitory domain of PMCA, both slowed Ca2+removal and raised baseline [Ca2+](i).
Although present in very low amounts in most plasma membranes (<0.1% of total membrane protein), [37] PMCA is highly conserved across species, indicating the essential role this pump plays in eukaryocytic function. Comparison of PMCA2 human and rat protein sequences, for example, shows >98% homology. [38] PMCA isomer [19] and splice variant expression [39] is strikingly regional in the rat brain. Splicing options in different isoforms change the affinities for Ca2+and calmodulin [40] and present unique sites for phosphorylation. Alternate splicing thus may be a tool for regional regulation of the Ca2+pump. Interestingly, the two splice variants of PMCA2 show a very high affinity for Ca2+and thus tend to maintain a lower free cytosolic Ca2+level in cells where they are expressed. [40] This isomer, PMCA2, is confined to the central nervous system where it is strategically located in nerve terminals and synapses. [41,42] These findings suggest specific functions for enzyme isoforms and splice variants in neurons and may even augur isoform-splice variant differences in the susceptibility to anesthetic depression that may be enhanced by strategic location within the brain.
These observations may be relevant to the pharmacodynamics of inhalation anesthetics, because both gaseous and volatile agents specifically inhibit PMCA in brain synaptic membranes [3–5] and cultured cells of neural origin. [6] Our laboratory has also observed parallel decreases or increases in the anesthetic partial pressures needed to prevent movement in response to pain in animal models with synaptic PMCA pumping activity that is incidentally decreased (in diabetic, spontaneously hypertension, and aged rats)[7–10] or increased (in obese Zucker rats). [11] To determine if inhalation anesthetics actually interfere with neuronal Ca2+homeostasis, we did electrophysiologic and microfluorimetric studies in cultured mouse central neurons (cortical, spinal, and dorsal root ganglion). [12] We observed delayed repolarization and delayed restoration of [Ca2+]ito basal levels after NMDA stimulation with exposure to halothane, <or= to 1 MAC equivalent, at 37 [degree sign]C. In contrast, Bleakman et al. [43] reported that Ca2+dynamics were unchanged in septal neurons exposed to halothane, isoflurane, enflurane, or desflurane. The substantive difference between their study and ours was the experimental temperature (room vs. physiologic). Our finding reported here that effects on intracellular Ca2+dynamics induced by halothane, isoflurane, and sevoflurane occurred at 37 [degree sign]C but not at 21 [degree sign]C raises the issue of the importance of physiologic temperature in the study of anesthetic effects. It is interesting that other treatments (orthovanadate, high pH, and C28R2) do inhibit PMCA at 21 [degree sign]C. [34] 
Given the now-established role of PMCA in fine tuning resting [Ca (2+)]iand in removing physiologic Ca2+loads in neurons, [34–36] it seems likely that the increases in resting and postligand [Ca (2+)]iand the delay in Ca2+clearance that we observed with anesthetic exposure were in fact due to PMCA inhibition. Therefore it is of interest that the pharmacologic concentrations of halothane had no effect on neuronal smooth endoplasmic reticulum Ca2+-ATPaseactivity or the Na+-Ca2+exchanger, two regulators of [Ca2+]i. [4] Although Kosk-Kosicka and Roszczynska [44] and Fomitcheva and Kosk-Kosicka [45] observed complete inhibition of PMCA nucleotide hydrolysis in erythrocytes and brain synaptic membranes, their results are not incompatible with the much smaller degree of PMCA pump inhibition that we noted, given the high concentrations of anesthetics they used (see their letter of erratum [46]). We also used a different analytic technique, measurement of ion transport. In our hands, ATP hydrolysis was an unsatisfactory measure of PMCA activity in neural tissue, confounded perhaps by the presence of significant amounts of nontransporting calcium-dependent ATPase (ecto-ATPases). [47–49] 
Other important systems that effect neuronal [Ca2+]iare voltage- and ligand-gated calcium channels and intracellular calcium release channels. Evidence has accumulated indicating depression by volatile anesthetics of Ca2+influx through the plasma membrane. [50,51] For example, Puil et al. [52] found that isoflurane reduced the increase in [Ca2+]iresulting from glutamate-linked Ca2+influx, and Daniell [53] observed that halothane and enflurane (at 2 or 3 MAC concentrations) inhibited hippocampal NMDA-receptor responses. Takenoshita and Steinbach [54] noted reduction by halothane of low-voltage-activated Ca2+currents in rat sensory neurons, and Study [55] reported inhibition by isoflurane of multiple voltage-gated Ca2+currents in hippocampal neurons. Miao et al. [56] found that isoflurane, enflurane, and halothane diminished Ca2+transients and calcium concentration in isolated brain synaptosomes, effects they concluded were compatible with depression of voltage-gated channels. MacIver et al. [57] observed temperature-independent anesthetic depression of glutamate-mediated excitatory postsynaptic potential amplitudes in hippocampal slices and proposed depression of Ca2+entry via voltage-gated channels as a likely mechanism of this effect. On the other hand, Pearce [58] provided evidence that halothane did not block voltage-sensitive channels in hippocampal slices. A general finding of studies of Ca2+channels is that anesthetics reduce Ca2+currents and Ca2+influx into cells. Thus a predicted effect would be reduction of [Ca2+]i, [24] a change contrary to that seen by Daniell. [53] Similarly, Mody et al. [59] provided evidence that halothane increases [Ca2+]iin hippocampal brain slices, possibly by stimulating release of intraneuronally stored calcium. Bickler et al. [60] studied isoflurane effects on Ca2+dynamics in hippocampal slices and noted depression of NMDA-mediated Ca2+influx at temperatures ranging from 28–39 [degree sign]C. They also noted, however, that isoflurane increased basal [Ca2+]iand did so to an increasing degree at higher brain slice temperatures, with mean basal [Ca2+]imore than three times greater at 37 [degree sign]C than at 28 [degree sign]C.
It is clear that among the multiple effects reported for volatile anesthetics there is a subset of multiple effects reported on cytosolic calcium regulators, along with certain inconsistencies as we have noted. It is also clear that anesthetics inhibit PMCA in neural cells, but can this effect be translated into the perturbations of [Ca2+]ithat we have observed? Our studies show that the net effect of anesthetics on intact neural cells maintained at 37 [degree sign]C is to increase [Ca2+]i, a finding that is consistent with inhibition of PMCA and in keeping with the findings of Benham et al. [34] and Werth et al. [36] 
What about reports indicating that anesthetics inhibit some Ca2+channels, sometimes with the expected associated result of a decrease in [Ca2+]i? Our studies support the idea that in circumstances wherein the anesthetic effects on PMCA are operational (available energy source, appropriate ionic constituents and physiologic temperature), effects on PMCA predominate, and [Ca2+]iwill increase rather than decrease. In the experiments of Miao et al., [56] PMCA was not a factor because no ATP was added. On the other hand, Winlow et al., [61] in a study of cultured molluscan neurons, found that although halothane and isoflurane depressed calcium currents, the unexpected net effect on [Ca2+]iwas an increase, and these results are compatible with our observations. Franks and Lieb, [24] noting that the literature indicates that nearly all systems studied are affected by anesthetics, emphasized the need for attention to pharmacologic concentrations and physiologic temperatures. We would add two other conditions, noted already: available energy source and ionic constituents.
If inhalation anesthetic interference with Ca2+dynamics in cultured primary neurons is a result of PMCA inhibition, a similar effect of anesthetics and a specific PMCA antagonist on Ca2+homeostasis should be demonstrable. Eosin may be such an antagonist, [62,63] and we have observed that eosin and halothane both prolong capsaicin-mediated Ca2+-dependentdepolarization in mouse adult dorsal root ganglion neurons. [12] Further, we found that infusion of eosin into the cerebroventricular system of rats produced a significant, reversible decrease in anesthetic requirements. [64] However, eosin has intrinsic fluorescence that interferes with microfluorimetric analyses. Because of this problem and a desire for greater specificity, we studied cells with specific blockade of PMCA isoform expression.
Cultured mouse embryonic cortical neurons maintained in media containing a 21 base antisense PMCA2 oligodeoxyribonucleotide, and with histochemical evidence of suppression of PMCA2 production, showed perturbation of Ca2+dynamics at one half the concentration of halothane required to produce a similar effect in control cells. (No halothane effect was observed at 21 [degree sign]C, however, even in these supersensitive neurons.) Rat pheochromocytoma cells transfected with an antisense-producing cDNA construct that blocked PMCA1 production manifested intracellular Ca2+perturbation when exposed to one tenth the concentration of halothane required to affect control cells. The fact that neural crest cells that are (1) of different tissue origin, (2) deficient in a different isoform of PMCA, (3) made deficient in isoform expression by different techniques, and (4) stimulated by a different ligand showed increased anesthetic sensitivity is especially noteworthy. These differences underscore the likelihood of a common site of anesthetic disturbance of calcium homeostasis in pheochromocytoma cells and neurons that is independent of these factors. It is interesting that pheochromocytoma cells transfected with antisense PMCA1 were particularly sensitive to halothane, compared with neurons with blocked PMCA2 production. Although extrapolation from one cell type to another is problematic, it is possible that the so-called housekeeping isoform, PMCA1, [19] may be more resistant to anesthetics than PMCA2, an isoform confined to the central nervous system. It is clear, however, that the results we obtained with these two cell models provide further evidence that anesthetic perturbation of intracellular Ca2+homeostasis is a result of effects on PMCA.
Finally, a fundamental test of any proposed site of general anesthetic action is its response to inhalation agents of widely different structure. For this reason, xenon, an inert element, is very important in the construction of any theory of the mechanism of anesthetics. Previously we reported that xenon inhibited PMCA activity in isolated neural membranes. [3,5,6] We have now examined the effect of this unusual anesthetic on Ca (2+) homeostasis in cultured neurons. If our postulate is correct, that inhibition of PMCA is an important mechanism of inhalation anesthetic action, we would expect to see perturbation of neuronal Ca2+dynamics with xenon exposure that is similar to that observed with volatile agents. Figure 10and Figure 11show such perturbation by xenon. This common Ca2+response of neurons to xenon and to potent anesthetics, along with the findings of Benham et al. [34] and Werth et al., [36] would also appear to dismiss a systematic, nonspecific, toxic effect of volatile agents on cell membranes as the cause of [Ca2+]ichanges.
Together the observations reported in this and preceding publications, along with recent information regarding the function and location of PMCA in the central nervous system, provide consistent evidence for an important role for this calcium pump in the production of the anesthetic state. Proposing a single molecule as a principal anesthetic target may appear simplistic given current knowledge of the complexity of the central nervous system and the multiple effects of anesthetics. We offer our experimental evidence fully aware that the complete story is still untold.
The authors thank Professor Ernesto Carafoli for providing anti-PMCA antibodies and Dr. David Piston for his assistance with experiments requiring use of the laser confocal microscope in the Vanderbilt University Medical Center Cell Imaging Core Resource, supported by CA68485 and DK20593.
REFERENCES
Rasmussen H, Barrett P, Zawalick W, Isales C, Stein P, Smallwood J, McCarthy R, Bollag W: Cycling of Ca2+across the plasma membrane as a mechanism for generating a Ca2+signal for cell activation. Ann N Y Acad Sci 1989; 568:73-80
Katz B, Miledi R: The release of acetylcholine from nerve endings by graded electric pulses. Proc R Soc Lond (Series B) 1967; 167:23-38
Franks JJ, Horn JL, Janicki PK, Singh G: Halothane, isoflurane, xenon, and nitrous oxide inhibit calcium, ATPase pump activity in rat brain synaptic plasma membranes. Anesthesiology 1995; 82:108-17
Franks JJ, Horn JL, Janicki PK, Singh G: Stable inhibition of brain synaptic plasma membrane calcium ATPase in rats anesthetized with halothane. Anesthesiology 1995; 82:118-28
Horn JL, Janicki PK, Franks JJ: Nitrous oxide and xenon enhance phospholipid-N-methylation in rat brain synaptic plasma membranes. Life Sci 1995; 56:PL455-60
Singh G, Janicki PK, Horn JL, Janson VE, Franks JJ: Inhibition of plasma membrane Ca2+-ATPasepump activity in cultured C6 glioma cells by halothane and xenon. Life Sci 1995; 56:PL219-24
Janicki PK, Horn JL, Singh G, Franks WT, Franks WT, Franks JJ: Diminished brain synaptic plasma membrane Ca2+-ATPaseactivity in rats with streptozocin-induced diabetes: Association with reduced anesthetic requirements. Life Sci 1994; 55:PL359-64
Janicki PK, Horn JL, Singh G, Janson VE, Franks WT, Franks JJ: Reduced anesthetic requirements, diminished brain plasma membrane Ca2+-ATPasepumping, and enhanced brain synaptic plasma membrane phospholipid methylation in diabetic rats: Effects of insulin. Life Sci 1995; 56:PL357-63
Horn JL, Janicki PK, Franks JJ: Diminished brain synaptic plasma membrane Ca2+-ATPaseactivity in spontaneously hypertensive rats: association with reduced anesthetic requirements. Life Sci 1995; 56:PL427-32
Horn JL, Janicki PK, Singh G, Wamil AW, Franks JJ: Reduced anesthetic requirements in aged rats: Association with altered brain synaptic plasma membrane Ca2+-ATPasepump with phospholipid methyltransferase I activities. Life Sci 1996; 59:PL263-8
Janicki PK, Horn JL, Singh G, Franks, WT, Janson VE, Franks JJ: Increased anesthetic requirements for isoflurane, halothane, enflurane and desflurane in obese Zucker rats are associated with insulin-induced stimulation of plasma membrane Ca2+-ATPase. Life Sci 1996; 59:PL269-75
Wamil AW, Franks JJ, Janicki PK, Horn JL, Franks WT: Halothane alters electrical activity and calcium dynamics in cultured mouse cortical, spinal cord, and dorsal root ganglion neurons. Neurosci Lett 1996; 216:93-6
Lachmann B, Armbruster S, Schairer W, Landstra M, Trouwborst A, van Daal GJ, Kusuma A, Erdmann W: Safety and efficacy of xenon in routine use as an inhalational anaesthetic. The Lancet 1990; 335:1413-5
Luttropp HH, Thomassoin R, Werner O: Clinical experience with minimal flow xenon anesthesia. Acta Anesthesiol Scand 1994;38:121-5
Wamil AW, McLean, MJ: Effects of temperature on limitation by MK-801 of firing of action potentials by spinal cord neurons in cell culture. Eur J Pharmacol 1993; 230:263-9
Brandt P, Neve RL: Expression of plasma membrane calcium-pumping ATPase mRNAs in developing rat brain and adult brain subregions: Evidence for stage-specific expression. J Neurochem 1992; 59:1566-9
Brandt P, Sisken J, Neve R, Vanaman TC: Blockade of plasma membrane calcium pumping ATPase isoform I impairs NGF-induced neurite extension in pheochromocytoma cells. Proc Natl Acad Sci U S A 1996; 93:13843-8
Preiano BS, Guerini D, Carafoli E: Expression and functional characterization of isoforms 4 of the plasma membrane calcium pump. Biochem 1996; 35:7946-53
Stauffer TP, Guerini D, Carafoli E: Tissue distribution of the four gene products of the plasma membrane Ca2+pump-A study using specific antibodies. J Biol Chem 1995; 270:12184-90
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 277:6010-25
Towbin H, Staehlin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci U S A 1979; 9:4350-4
Janicki PK, Erskine WAR, James MFM: High-performance liquid chromatographic method for the volatile anaesthetics halothane, isoflurane and enflurane in water and in physiological buffer solutions. J Chromatogr 1990; 518:250-3
Horn JL, Johnson R, Janicki PK, Franks JJ: Simplified method for measurement of halothane in solution: Comparison with gas chromatographic techniques [Abstract]. Anesthesiology 1995; 83:A936
Franks NP, Lieb WR: Selective actions of volatile general anaesthetics at molecular and cellular levels. Br J Anaesth 1993; 71:65-76
Kashimoto S, Furuya A, Oguchi T, Koshimizu M, Kumazawa T: The minimum alveolar concentration of sevoflurane in rats. Eur J Anaesth 1997; 14:359-61
Weast RC, Selby SM, Hodgman CD: Physical constants in inorganic compounds, CRC Handbook of Chemistry and Physics. Boca Raton, FL, CRC Press, 1964, pp B-200, B201, B-237
Miller KW, Paton WD, Smith EB: Physicochemical approaches to the mode of action of general anesthetics. Anesthesiology 1972; 36:339-51
Miller RJ: Calcium signaling in neurons. Trends Neurosci 1988; 11:415-9
Randall RD, Thayer SA: Glutamate-induced calcium transient triggers delayed calcium overload and neurotoxicity in rat hippocampal neurons. J Neurosci 1992; 12:1882-95
Thayer SA, Miller R: Regulation of the intracellular free calcium concentration in single rat dorsal root ganglion neurones in vitro. J Physiol 1990; 425:85-115
Tsien RW, Tsien RY: Calcium channels, stores, and oscillations. Annu Rev Cell Biol 1990; 6:715-60
Miller RJ: The control of neuronal Ca2+homeostasis. Prog Neurobiol 1991; 37:255-85
Carafoli E: Calcium pump of the plasma membrane. Phys Rev 1991; 71:129-53
Benham CD, Evans ML, McBain CJ: Ca2+efflux mechanisms following depolarization evoked calcium transients in cultured rat sensory neurones. J Physiol (Lond) 1992; 455:567-83
Bleakman D, Roback JD, Wainer BH, Miller RJ, Harrison NL: Calcium homeostasis in rat septal neurons in tissue culture. Brain Res 1993; 600:257-67
Werth JL, Usachev YM, Thayer SA: Modulation of calcium efflux from cultured rat dorsal root ganglion neurons. J Neurosci 1996; 16:1008-15
Knauf P, Proverbio F, Hoffman JF: Electrophoretic separation of different phosphoproteins associated with Ca-ATPase and Na, K-ATPase in human red cell ghosts. J Gen Physiol 1974; 63:324-36
Brandt P, Ibrahim E, Bruns GAP, Neve RL: Determination of the nucleotide sequence and chromosomal localization of the ATP2B2 gene encoding human Ca2+-pumpingATPase isoform PMCA2. Genomics 1992; 14:484-7
Filoteo AF, Elwess NL, Enyedi A, Caride A, Aung HH, Penniston JT: Plasma membrane Ca2+pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 1997; 272:23741-7
Elwess NL, Filoteo AG, Enyedi A, Penniston JT: Plasma Membrane Ca2+Pump Isoforms 2a and 2b are unusually responsive to calmodulin and Ca2+. J Biol Chem 1997; 272:17981-6
Fujii JT, Su FT, Woodbury DJ, Kurpakus M, Hu XJ, Pourcho R: Plasma membrane calcium ATPase in synaptic terminals of chick Edinger-Westphal neurons. Brain Res 1996; 734:193-202
Hillman DE, Chen S, Bing R, Penniston JT, Llinas R: Ultrastructural localization of the plasmalemmal calcium pump in cerebellar neurons. Neuroscience 1996; 72:315-24
Bleakman D, Jones MV, Harrison NL: The effects of four general anesthetic on intracellular [Ca2+]iin cultured rat hippocampal neurons. Neuropharmacol 1995; 34:541-51
Kosk-Kosicka D, Roszczynska G: Inhibition of plasma membrane Ca2+-ATPaseby volatile anesthetics. Anesthesiology 1993; 79:774-80
Fomitcheva I, Kosk-Kosicka D: Volatile anesthetics selectively inhibit the Ca2+transporting ATPase in neuronal and erythrocyte plasma membranes. Anesthesiology 1996; 84:1189-95
Kosk-Kosicka D: Inhibition of plasma membrane Ca2+ATPase by volatile anesthetics [Letter]. Anesthesiology 1996; 85:1211-2
Nagy AK, Shuster TA, Delgado-Escueta AV: Ecto-ATPase of mammalian synaptosomes: Identification and enzymic characterization. J Neurochem 1986; 47:976-86
Cardy JD, Firth JA: Adenosine triphosphate-lead histochemical reactions in ependymal epithelia of murine brains do not represent calcium transport adenosine triphosphatase. Histochem J 1993; 25:319-24
Kittel A, Bacsy E: Presynaptic ecto- and postsynaptic endocalcium-adenosine-triphosphatases in synaptosomes: Doubts about biochemical interpretation of localization. Int J Dev Neurosci 1994; 12:207-11
Hirota K, Lambert DG: Voltage-sensitive Ca2+channels and anaesthesia [Editorial]. Br J Anaesth 1996; 76:344-6
Pocock G, Richards CD: Excitatory and inhibitory synaptic mechanisms in anaesthesia. Br J Anaesth 1996; 71:134-47
Puil E, el-Beheiry H, Baimbridge KG: Anesthetic effects on glutamate-stimulated increase in intraneuronal calcium. J Pharmacol Exp Ther 1990; 255:955-61
Daniell LC: Effect of volatile general anesthetics and n-alcohols on glutamate-stimulated increases in calcium ion flux in hippocampal membrane vesicles. Pharmacology 1995; 50:154-61
Takenoshita M, Steinbach JH: Halothane blocks low voltage-activated calcium current in rat sensory neurons. J Neurosci 1991; 11:1404-12
Study RE: Isoflurane inhibits multiple voltage-gated calcium currents in hippocampal pyramidal neurons. Anesthesiology 1994; 81:104-16
Miao N, Frazer MJ, Lynch C III: Volatile anesthetics depress Ca2+transients and glutamate release in isolated cerebral synaptosomes. Anesthesiology 1995; 83:593-603
MacIver MB, Mikulec AA, Amagasu SM, Monroe FA: Volatile anesthetics depress glutamate transmission via presynaptic actions. Anesthesiology 1996; 85:823-34
Pearce RA: Volatile anaesthetic enhancement of paired-pulse depression investigated in the rate hippocampus in vitro. J Physiol (Lond) 1996; 492:823-40
Mody I, Tanelian DL, MacIver B: Halothane enhances tonic neuronal inhibition by elevating intracellular calcium, Brain Res 1991; 538:319-23
Bickler PE, Buck LT, Hansen BM: Effects of isoflurane and hypothermia on glutamate receptor-mediated calcium influx in brain slices. Anesthesiology 1994; 81:1461-9
Winlow W, Hopkins PM, Moghadam HF, Ahmed IA, Yat T: Multiple cellular and subcellular actions of general anaesthetics on cultured molluscan neurones. Acta Biol Hung 1995; 46:381-93
Gatto C, Malonic MA: Inhibition of the red blood cell calcium pump by eosin and other fluorescein analogues. Am J Physiol 1993; 264:C1577-86
Gatto C, Hale CC, Xu W, Malonic MA: Eosin, a potent inhibitor of the plasma membrane Ca pump, does not inhibit the cardiac NaCa exchanger. Biochemistry 1995; 34:965-72
Horn J-L, Janicki PK, Wamil A, Franks JJ: Eosin, a plasma membrane Ca2+-ATPasepump inhibitor, reduces anesthetic requirements in rats [Abstract]. Anesthesiology 1996; 85:A682
Figure 1. Analysis of the time course of Ca2+-relatedfluorescence in a mouse embryonic cortical neuron. Sequential digitized images of a microscopic field of cells previously loaded with Flu3-AM (a calcium-sensitive dye) were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA; 10-4M) and stored on a zip drive. Subsequently, single cells in the field were chosen at random and magnified for analysis. Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated (control) neuron and the upper from the same cell after a 10-min superfusion with an inhalation anesthetic solution (0.3 mM halothane). Each curve is derived from a computer conversion of fluorescence intensity of 64 sequential images to scalar values of fluorescence. Typical images are shown in the Figure toillustrate how points on the graph are obtained. The abscissa shows time in seconds (s), and the ordinate indicates arbitrary units (au) of fluorescence provided by the LSM410-associated software. As is clear in both sequences of images, Ca2+-associatedfluorescence is increased after NMDA application, and calcium clearance subsequently returns to prestimulation levels.
Figure 1. Analysis of the time course of Ca2+-relatedfluorescence in a mouse embryonic cortical neuron. Sequential digitized images of a microscopic field of cells previously loaded with Flu3-AM (a calcium-sensitive dye) were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA; 10-4M) and stored on a zip drive. Subsequently, single cells in the field were chosen at random and magnified for analysis. Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated (control) neuron and the upper from the same cell after a 10-min superfusion with an inhalation anesthetic solution (0.3 mM halothane). Each curve is derived from a computer conversion of fluorescence intensity of 64 sequential images to scalar values of fluorescence. Typical images are shown in the Figure toillustrate how points on the graph are obtained. The abscissa shows time in seconds (s), and the ordinate indicates arbitrary units (au) of fluorescence provided by the LSM410-associated software. As is clear in both sequences of images, Ca2+-associatedfluorescence is increased after NMDA application, and calcium clearance subsequently returns to prestimulation levels.
Figure 1. Analysis of the time course of Ca2+-relatedfluorescence in a mouse embryonic cortical neuron. Sequential digitized images of a microscopic field of cells previously loaded with Flu3-AM (a calcium-sensitive dye) were captured just before and after stimulation of calcium influx by N-methyl-D-aspartate (NMDA; 10-4M) and stored on a zip drive. Subsequently, single cells in the field were chosen at random and magnified for analysis. Shown are two time courses of Ca2+-associatedfluorescence, the lower obtained from an untreated (control) neuron and the upper from the same cell after a 10-min superfusion with an inhalation anesthetic solution (0.3 mM halothane). Each curve is derived from a computer conversion of fluorescence intensity of 64 sequential images to scalar values of fluorescence. Typical images are shown in the Figure toillustrate how points on the graph are obtained. The abscissa shows time in seconds (s), and the ordinate indicates arbitrary units (au) of fluorescence provided by the LSM410-associated software. As is clear in both sequences of images, Ca2+-associatedfluorescence is increased after NMDA application, and calcium clearance subsequently returns to prestimulation levels.
×
Figure 2. Halothane alteration of Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response of a representative cultured mouse embryonic cortical neuron before, during, and after exposure to 0.30 mM halothane. The abscissa shows seconds (s), and the ordinate shows Ca2+-relatedfluorescence in arbitrary units (au). NMDA (10-4M) was applied for 3 s, indicated by horizontal bars, after 10 min of superfusion with Dulbecco's modified buffer (see text), buffer with halothane, or buffer alone. Cells were maintained at 37 [degree sign]C.
Figure 2. Halothane alteration of Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response of a representative cultured mouse embryonic cortical neuron before, during, and after exposure to 0.30 mM halothane. The abscissa shows seconds (s), and the ordinate shows Ca2+-relatedfluorescence in arbitrary units (au). NMDA (10-4M) was applied for 3 s, indicated by horizontal bars, after 10 min of superfusion with Dulbecco's modified buffer (see text), buffer with halothane, or buffer alone. Cells were maintained at 37 [degree sign]C.
Figure 2. Halothane alteration of Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response of a representative cultured mouse embryonic cortical neuron before, during, and after exposure to 0.30 mM halothane. The abscissa shows seconds (s), and the ordinate shows Ca2+-relatedfluorescence in arbitrary units (au). NMDA (10-4M) was applied for 3 s, indicated by horizontal bars, after 10 min of superfusion with Dulbecco's modified buffer (see text), buffer with halothane, or buffer alone. Cells were maintained at 37 [degree sign]C.
×
Figure 3. Summary of the temperature-dependent effects of halothane on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cortical neurons. Each cell was stimulated by N-methyl-D-aspartate (NMDA) after sequential superfusion for 10 min with buffer (control 1), buffer with 0.30 mM halothane, and buffer (control 2). Experimental conditions were as described in Figure 2. The ordinates give time and relative Ca2+-associated fluorescence, the latter normalized so that initial, prehalothane baseline is unity. Subsequent pre-NMDA baseline (BL) and post-NMDA peak (PK) fluorescence levels are shown as fractional increases above initial baseline. Columns show mean values with 99% confidence limits. Asterisks denote significant prolongation of Ca2+clearance time and elevation of baseline and peak [Ca2+]iin cells exposed to halothane at 37 [degree sign]C (P < 0.01, n = 22). No halothane effects were seen at 21 [degree sign]C (n = 8).
Figure 3. Summary of the temperature-dependent effects of halothane on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cortical neurons. Each cell was stimulated by N-methyl-D-aspartate (NMDA) after sequential superfusion for 10 min with buffer (control 1), buffer with 0.30 mM halothane, and buffer (control 2). Experimental conditions were as described in Figure 2. The ordinates give time and relative Ca2+-associated fluorescence, the latter normalized so that initial, prehalothane baseline is unity. Subsequent pre-NMDA baseline (BL) and post-NMDA peak (PK) fluorescence levels are shown as fractional increases above initial baseline. Columns show mean values with 99% confidence limits. Asterisks denote significant prolongation of Ca2+clearance time and elevation of baseline and peak [Ca2+]iin cells exposed to halothane at 37 [degree sign]C (P < 0.01, n = 22). No halothane effects were seen at 21 [degree sign]C (n = 8).
Figure 3. Summary of the temperature-dependent effects of halothane on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cortical neurons. Each cell was stimulated by N-methyl-D-aspartate (NMDA) after sequential superfusion for 10 min with buffer (control 1), buffer with 0.30 mM halothane, and buffer (control 2). Experimental conditions were as described in Figure 2. The ordinates give time and relative Ca2+-associated fluorescence, the latter normalized so that initial, prehalothane baseline is unity. Subsequent pre-NMDA baseline (BL) and post-NMDA peak (PK) fluorescence levels are shown as fractional increases above initial baseline. Columns show mean values with 99% confidence limits. Asterisks denote significant prolongation of Ca2+clearance time and elevation of baseline and peak [Ca2+]iin cells exposed to halothane at 37 [degree sign]C (P < 0.01, n = 22). No halothane effects were seen at 21 [degree sign]C (n = 8).
×
Figure 4. Ca2+clearance (A) and baseline and peak relative [Ca2+]i(B) in cortical neurons exposed at 37 [degree sign]C to isoflurane or sevoflurane. N-methyl-D-aspartate was applied after sequential superfusion for 10 min or more with buffer (control 1), buffer with either 0.35 mM isoflurane or 0.50 mM sevoflurane, and buffer (control 2). Both agents prolonged Ca2+clearance and elevated baseline and peak [Ca2+]i(P < 0.01, n = 10 for isoflurane and n = 8 for sevoflurane).
Figure 4. Ca2+clearance (A) and baseline and peak relative [Ca2+]i(B) in cortical neurons exposed at 37 [degree sign]C to isoflurane or sevoflurane. N-methyl-D-aspartate was applied after sequential superfusion for 10 min or more with buffer (control 1), buffer with either 0.35 mM isoflurane or 0.50 mM sevoflurane, and buffer (control 2). Both agents prolonged Ca2+clearance and elevated baseline and peak [Ca2+]i(P < 0.01, n = 10 for isoflurane and n = 8 for sevoflurane).
Figure 4. Ca2+clearance (A) and baseline and peak relative [Ca2+]i(B) in cortical neurons exposed at 37 [degree sign]C to isoflurane or sevoflurane. N-methyl-D-aspartate was applied after sequential superfusion for 10 min or more with buffer (control 1), buffer with either 0.35 mM isoflurane or 0.50 mM sevoflurane, and buffer (control 2). Both agents prolonged Ca2+clearance and elevated baseline and peak [Ca2+]i(P < 0.01, n = 10 for isoflurane and n = 8 for sevoflurane).
×
Figure 5. Immunofluorescent staining of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Cells were immunostained with isoform-specific rabbit antibody, which reacts only with PMCA2. (Left) Control neurons showing PMCA2 specific immunostaining. (Right) Reduced immunostaining intensity resulting from deficiency in PMCA2 (x1,200).
Figure 5. Immunofluorescent staining of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Cells were immunostained with isoform-specific rabbit antibody, which reacts only with PMCA2. (Left) Control neurons showing PMCA2 specific immunostaining. (Right) Reduced immunostaining intensity resulting from deficiency in PMCA2 (x1,200).
Figure 5. Immunofluorescent staining of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Cells were immunostained with isoform-specific rabbit antibody, which reacts only with PMCA2. (Left) Control neurons showing PMCA2 specific immunostaining. (Right) Reduced immunostaining intensity resulting from deficiency in PMCA2 (x1,200).
×
Figure 6. Increased anesthetic sensitivity of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Experimental conditions were the same as described in Figure 2. Shown are microfluorimetric recordings of the Ca2+response in wild-type (top row), antisense oligodeoxyribonucleotide-treated (middle row), and scrambled base-treated (bottom row) neurons. N-methyl-D-aspartate was applied after 10 min of sequential superfusion with buffer, buffer with 0.15 mM halothane, buffer, buffer with 0.30 mM halothane, and again with buffer.
Figure 6. Increased anesthetic sensitivity of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Experimental conditions were the same as described in Figure 2. Shown are microfluorimetric recordings of the Ca2+response in wild-type (top row), antisense oligodeoxyribonucleotide-treated (middle row), and scrambled base-treated (bottom row) neurons. N-methyl-D-aspartate was applied after 10 min of sequential superfusion with buffer, buffer with 0.15 mM halothane, buffer, buffer with 0.30 mM halothane, and again with buffer.
Figure 6. Increased anesthetic sensitivity of embryonic mouse cortical neurons with oligodeoxyribonucleotide blockade of PMCA2 expression. Experimental conditions were the same as described in Figure 2. Shown are microfluorimetric recordings of the Ca2+response in wild-type (top row), antisense oligodeoxyribonucleotide-treated (middle row), and scrambled base-treated (bottom row) neurons. N-methyl-D-aspartate was applied after 10 min of sequential superfusion with buffer, buffer with 0.15 mM halothane, buffer, buffer with 0.30 mM halothane, and again with buffer.
×
Figure 7. Summary of effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-treated neurons. These same Ca2+parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).
Figure 7. Summary of effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-treated neurons. These same Ca2+parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).
Figure 7. Summary of effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (n = 6), antisense PMCA2 oligodeoxyribonucleotide-treated (n = 8), and scrambled base-treated (n = 6) neurons. Each cell was stimulated with N-methyl-D-aspartate after sequential superfusion for 10 min or more with buffer (c1), buffer with 0.15 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.5 and 1 minimum alveolar concentration prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-treated neurons. These same Ca2+parameters were affected in wild-type and scrambled base-treated neurons by the higher but not the low halothane concentration. *Values different from all other groups within each cell type. dagger A value different from all other groups within the antisense-treated cells (P < 0.01).
×
Figure 8. Western immunoblot analysis of PMCA isoforms in plasma membranes of transfected pheochromocytoma cells. Column 1 shows purified erythrocytic PMCA1 and PMCA4, and column 2 shows rat cortical synaptic plasma membranes, for reference. Column 3 shows membrane isolates from cells transfected with PMCA1 sense cDNA (RSV14), column 4 shows isolates from cells transfected with PMCA1 antisense cDNA (RSV9), and column 5 shows isolates from wild-type (PC6). Rabbit antisera 1N, 2N, and 3N react with PMCA isoforms 1, 2, and 3. Marker protein positions are indicated along the left margin.
Figure 8. Western immunoblot analysis of PMCA isoforms in plasma membranes of transfected pheochromocytoma cells. Column 1 shows purified erythrocytic PMCA1 and PMCA4, and column 2 shows rat cortical synaptic plasma membranes, for reference. Column 3 shows membrane isolates from cells transfected with PMCA1 sense cDNA (RSV14), column 4 shows isolates from cells transfected with PMCA1 antisense cDNA (RSV9), and column 5 shows isolates from wild-type (PC6). Rabbit antisera 1N, 2N, and 3N react with PMCA isoforms 1, 2, and 3. Marker protein positions are indicated along the left margin.
Figure 8. Western immunoblot analysis of PMCA isoforms in plasma membranes of transfected pheochromocytoma cells. Column 1 shows purified erythrocytic PMCA1 and PMCA4, and column 2 shows rat cortical synaptic plasma membranes, for reference. Column 3 shows membrane isolates from cells transfected with PMCA1 sense cDNA (RSV14), column 4 shows isolates from cells transfected with PMCA1 antisense cDNA (RSV9), and column 5 shows isolates from wild-type (PC6). Rabbit antisera 1N, 2N, and 3N react with PMCA isoforms 1, 2, and 3. Marker protein positions are indicated along the left margin.
×
Figure 9. Effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca2+parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).
Figure 9. Effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca2+parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).
Figure 9. Effects of halothane on Ca2+clearance (A) and on baseline and peak relative [Ca2+]i(B) in wild-type (PC6, n = 6), antisense PMCA1-transfected (RSV-9, n = 8) pheochromocytoma cells and sense PMCA1-transfected (RSV-14, n = 6) pheochromocytoma cells. Each cell was stimulated with bradykinin (10-3M applied for 3 s) after sequential superfusion for 20 min with buffer (c1), buffer with 0.03 mM halothane (h1), buffer (c2), buffer with 0.30 mM halothane (h2), and buffer (c3). Halothane at concentrations equivalent to 0.1 MAC prolonged Ca2+clearance and elevated baseline and peak [Ca2+]iin antisense-transfected cells with blocked production of PMCA1, but not in wild-type and sense-transfected cells. These same Ca2+parameters were altered in all three cell types by 0.30 mM halothane. *Values different from all other groups within each cell type. [dagger]A value different from all other groups within the antisense-transfected cells (P < 0.01).
×
Figure 10. Xenon effects on Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response in a representative cultured mouse embryonic cortical neuron exposed to increasing concentrations of xenon (all less than the minimum alveolar concentration [MAC]). NMDA was applied after 10 min of sequential superfusion with buffers equilibrated with dry gas mixtures of 20% oxygen and xenon-nitrogen in ratios providing xenon partial pressures ranging from 0–80% of 1 atm. Calculated maximum MAC-equivalent values of xenon solutions were 0.20, 0.40, 0.60, and 0.80, as described in the text.
Figure 10. Xenon effects on Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response in a representative cultured mouse embryonic cortical neuron exposed to increasing concentrations of xenon (all less than the minimum alveolar concentration [MAC]). NMDA was applied after 10 min of sequential superfusion with buffers equilibrated with dry gas mixtures of 20% oxygen and xenon-nitrogen in ratios providing xenon partial pressures ranging from 0–80% of 1 atm. Calculated maximum MAC-equivalent values of xenon solutions were 0.20, 0.40, 0.60, and 0.80, as described in the text.
Figure 10. Xenon effects on Ca2+homeostasis in cortical neurons. Shown are confocal microfluorimetric tracings of the N-methyl-D-aspartate (NMDA) response in a representative cultured mouse embryonic cortical neuron exposed to increasing concentrations of xenon (all less than the minimum alveolar concentration [MAC]). NMDA was applied after 10 min of sequential superfusion with buffers equilibrated with dry gas mixtures of 20% oxygen and xenon-nitrogen in ratios providing xenon partial pressures ranging from 0–80% of 1 atm. Calculated maximum MAC-equivalent values of xenon solutions were 0.20, 0.40, 0.60, and 0.80, as described in the text.
×
Figure 11. Summary of effects of different concentrations of xenon on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cultured cortical neurons. Exposure conditions and procedures are described in Figure 10. Columns in part A give 95% confidence limits, whereas those in part B give 99% confidence limits. Differences among responses to varying xenon concentrations are defined by line segments adjacent to each response parameter. Statistical significance, based on multifactor analysis of variance, is indicated when segments are discontinuous and do not overlap (P < 0.05 for panel A and P < 0.01 for panel B, n = 10). Nonparametric testing (Kruskal-Wallis) confirmed the differences observed in Ca+-associatedfluorescence (panel B) and indicated that median clearance times in cells exposed to xenon minimum alveolar concentration-equivalent values of 0.40, 0.60, and 0.80 differed significantly from each other and from 0 and 0.20 (P < 0.01). Thus a clear pattern of increased perturbation of Ca2+dynamics with increasing xenon concentrations was observed.
Figure 11. Summary of effects of different concentrations of xenon on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cultured cortical neurons. Exposure conditions and procedures are described in Figure 10. Columns in part A give 95% confidence limits, whereas those in part B give 99% confidence limits. Differences among responses to varying xenon concentrations are defined by line segments adjacent to each response parameter. Statistical significance, based on multifactor analysis of variance, is indicated when segments are discontinuous and do not overlap (P < 0.05 for panel A and P < 0.01 for panel B, n = 10). Nonparametric testing (Kruskal-Wallis) confirmed the differences observed in Ca+-associatedfluorescence (panel B) and indicated that median clearance times in cells exposed to xenon minimum alveolar concentration-equivalent values of 0.40, 0.60, and 0.80 differed significantly from each other and from 0 and 0.20 (P < 0.01). Thus a clear pattern of increased perturbation of Ca2+dynamics with increasing xenon concentrations was observed.
Figure 11. Summary of effects of different concentrations of xenon on Ca (2+) clearance (A) and on baseline and peak Ca2+-relatedfluorescence (B) in cultured cortical neurons. Exposure conditions and procedures are described in Figure 10. Columns in part A give 95% confidence limits, whereas those in part B give 99% confidence limits. Differences among responses to varying xenon concentrations are defined by line segments adjacent to each response parameter. Statistical significance, based on multifactor analysis of variance, is indicated when segments are discontinuous and do not overlap (P < 0.05 for panel A and P < 0.01 for panel B, n = 10). Nonparametric testing (Kruskal-Wallis) confirmed the differences observed in Ca+-associatedfluorescence (panel B) and indicated that median clearance times in cells exposed to xenon minimum alveolar concentration-equivalent values of 0.40, 0.60, and 0.80 differed significantly from each other and from 0 and 0.20 (P < 0.01). Thus a clear pattern of increased perturbation of Ca2+dynamics with increasing xenon concentrations was observed.
×