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Clinical Science  |   February 2000
The Pharmacodynamic Effect of a Remifentanil Bolus on Ventilatory Control
Author Affiliations & Notes
  • H. Daniel Babenco, M.D.
    *
  • Pattilyn F. Conard, C.R.N.A., M.A.
  • Jeffrey B. Gross, M.D.
  • *Senior Resident in Anesthesiology. †Instructor in Anesthesiology. ‡Professor of Anesthesiology and Pharmacology.
Article Information
Clinical Science
Clinical Science   |   February 2000
The Pharmacodynamic Effect of a Remifentanil Bolus on Ventilatory Control
Anesthesiology 2 2000, Vol.92, 393. doi:
Anesthesiology 2 2000, Vol.92, 393. doi:
REMIFENTANIL is a rapidly metabolized intravenous opioid that is well-suited for situations necessitating rapid changes in anesthetic depth or when the postoperative requirement for analgesia is of limited duration. The pharmacokinetic properties of remifentanil, based on the exponential decay in plasma concentration after a continuous infusion, are well-established. 1–3 Context-sensitive half-times, based on these parameters, indicate that remifentanil concentrations decrease rapidly, regardless of the rate or duration of remifentanil infusion. 4 The decrease in analgesia and ventilatory depression after discontinuation of remifentanil parallel the decrease in plasma concentration. 4 However, when an initial intravenous bolus dose of remifentanil is administered, the onset of analgesia and ventilatory depression is not instantaneous because it takes time for remifentanil to cross the blood–brain barrier and reach the “effect sites” within the central nervous system. We designed this study to characterize the time course of ventilatory depression after a typical loading dose of remifentanil.
Materials and Methods
Eight nonsmoking healthy volunteers (two women and six men), ranging in age from 23 to 31 yr and in weight from 61 to 102 kg, consented to participate in this Internal Review Board–approved study. Subjects abstained from alcohol and caffeine for 24 h and took nothing by mouth for at least 8 h before the start of the study. To minimize the effect of auditory stimulation during ventilatory testing, the supine subjects listened to quiet classical music via  headphones. We monitored blood pressure via  forearm cuff, electrocardiography (ECG), and oxygen saturation as measured by pulse oximetry (SpO2; Ohmeda Boulder, CO; 3700, fast mode). A Datex (Helsinki, Finland) Capnomac I calibrated with three reference mixtures of carbon dioxide in oxygen continuously monitored end-tidal partial pressure of carbon dioxide (PETCO2) and fractional inspired oxygen tension (FIO2). Subjects breathed via  mask from a to-and-fro circuit with variable carbon dioxide absorption, enabling us to keep PETCO2constant to within ± 1 mmHg, as previously described. 5 A Hans-Rudolph (Kansas City, MO) 3700 heated pneumotachograph with a Validyne (Northridge, CA) DP45 differential pressure transducer and electronic integrator determined ventilatory volumes at body temperature pressure, saturated (BTPS). Before each set of measurements we performed a three-point volume calibration and linearity check using a Collins (Braintree, MA) 3200 3-l super syringe. An analog-to-digital converter and a computer recorded breath-by-breath measurements of minute ventilation (V̇E), tidal volume (VT), respiratory rate, SPO2, and PETCO2.
To determine the steady state ventilatory response to carbon dioxide, subjects breathed hyperoxic mixtures (FIO2> 0.6) of oxygen in nitrogen with PETCO2held constant at approximately 46 or 56 mmHg for alternate subjects; these carbon dioxide tensions were chosen because they typically lie on the linear portion of the carbon dioxide ventilatory response curve. After keeping PETCO2constant for a 6-min equilibration period, we recorded baseline ventilation and PETCO2for an additional 2-min. While data collection continued, we administered 0.5 μg/kg intravenous remifentanil over 5 s. We continued to record breath-by-breath changes in ventilation for the next 20 min while adjusting the flow of gas through the carbon dioxide absorber to maintain PETCO2as close as possible to the desired value (typically within ± 1 or 2 mmHg). Small volumes (≈ 300 ml/min) of oxygen were added to the circuit as needed to maintain a constant circuit volume. After a 2-h recovery period, we repeated the steady state and hypoxic ventilatory measurements at the alternate PETCO2(56 or 46 mmHg), whichever was not used previously.
Data Analysis
Because of the inherent variability of breath-by-breath ventilatory measurements, we used five-breath average values of V̇E, VT, and PETCO2throughout the data analysis. Starting at the time of injection (T = 0), we evaluated ventilation data at 30-s intervals for 20 min. For each subject, we determined two-point carbon dioxide ventilatory response curves (V̇Evs.  PETCO2) at each 30-s interval after remifentanil injection: A line was drawn between the point representing V̇Eand PETCO2at a given time during the first “run” and the point describing these variables at the corresponding time during the second “run” (fig. 1). 6 To summarize the data, we described each of these lines in terms of its slope and the V̇Eat PETCO2= 50 mmHg (V̇E50) at each time after remifentanil injection. (These values represent the “gain” and “set-point,” respectively, of the ventilatory control system.) Similar computations using VTversus  PETCO2enabled us to compute the VTat PETCO2= 50 mmHg (VT50) at each time after injection. Repeated-measures analysis of variance (subjects × times) followed by protected least significant difference tests determined the times at which ventilatory variables were significantly lower than the baseline, with P  < 0.05 indicating statistical significance.
Fig. 1. Constructed carbon dioxide response curves before (time = 0) and at selected times after injection of 0.5 μg/kg remifentanil. These curves are based on mean values for V̇Eand PETCO2at each time point.
Fig. 1. Constructed carbon dioxide response curves before (time = 0) and at selected times after injection of 0.5 μg/kg remifentanil. These curves are based on mean values for V̇Eand PETCO2at each time point.
Fig. 1. Constructed carbon dioxide response curves before (time = 0) and at selected times after injection of 0.5 μg/kg remifentanil. These curves are based on mean values for V̇Eand PETCO2at each time point.
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To model the pharmacodynamics of remifentanil-induced ventilatory depression, we used published pharmacokinetic data for remifentanil to estimate the subjects’ remifentanil blood concentration as a function of time after injection. 3 We assumed first-order transfer kinetics between the central compartment and the effect site, and a sigmoidal relation between effect-site concentration Ceand the observed effect on V̇E50, MATH 1where V̇E500is the baseline ventilation, VE50MINis the V̇Ecorresponding to maximum remifentanil-induced ventilatory depression (constrained to be ≥ 0), EC50is the plasma concentration causing 50% depression of V̇E50, and γ is a dimensionless shape factor which determines the “steepness” of the dose–response curve. By using nonlinear least-squares estimation, 7 1we minimized the least-squares difference between the ventilatory effect predicted based on the pharmacokinetic–pharmacodynamic model and observed effect (method of collapsing hysteresis loops). 3 This analysis yielded values for the onset rate constant keo, EC50, and γ.
We performed the analysis in two ways:1 On a subject-by-subject basis, we estimated the pharmacodynamic parameters; from these individual estimates we computed overall mean values with confidence limits. 2 We computed a single value for the pharmacodynamic parameters based on the mean values of the V̇E50 for all subjects versus  the time data. We used the Pearson product moment correlation for individual and pooled data to determine the degree to which our model predicted the variations in the observed ventilatory data.
In addition, we computed the offset half-time (T2) for remifentanil-induced depression of V̇E50 using nonlinear regression to estimate a standard, biexponential model:MATH 2
All data are shown as the mean [95% confidence intervals], with P  < 0.05 indicating statistical significance.
Results
After remifentanil administration, all subjects were mildly sedated, responsive to verbal command in normal tone, and had mild ptosis or a “glazed” appearance of the eyes. This corresponds to a change from 5 to 4 on the Observers Assessment of Alertness/Sedation Scale (OAA/S). 8 None of the subjects lost consciousness or became apneic during the study, and no subject had an Observers Assessment of Alertness/Sedation Scale score lower than 4 at any time.
After the injection of remifentanil, VEdecreased at low (≈ 46 mmHg) and high (≈ 56 mmHg) carbon dioxide tensions (fig. 1). The decrease in the slope of the carbon dioxide response achieved significance within 30 s, decreasing from 0.99 [0.72 to 1.26] to 0.58 l · min−1· mmHg−1[0.28 to 0.89] (P  < 0.05) before reaching its nadir of 0.27 l · min−1· mmHg−1[−0.12 to 0.66] (P  < 0.001) 2 min after injection (fig. 2). The transient increase in slope between 4 and 5 min resulted from a slightly greater increase at the high carbon dioxide level, as compared with the corresponding increase at low carbon dioxide; however the position of the carbon dioxide response remained below baseline. Slope remained significantly depressed for approximately 9 min after remifentanil injection, but, within 15 min, it returned to baseline.
Fig. 2. Time course of the slope of the ventilatory response to carbon dioxide after injection of remifentanil 0.5 μg/kg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 2. Time course of the slope of the ventilatory response to carbon dioxide after injection of remifentanil 0.5 μg/kg. Values below the dotted line differ significantly (P 
	< 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 2. Time course of the slope of the ventilatory response to carbon dioxide after injection of remifentanil 0.5 μg/kg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
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Similarly, V̇E50 decreased significantly within 30 s of injection from a baseline of 12.9 [9.8 to 15.9] to 10.5 l/min [7.8 to 13.2], reaching a nadir of 6.1 l/min [4.8 to 7.4] 2.5 min after injection. As shown in figure 3, V̇E50 remained significantly lower than baseline for approximately 12 min after remifentanil injection; within 15 min, V̇E50 returned to the preremifentanil baseline. The decrease in V̇E50 resulted primarily from a decrease in VT(VT50), as shown in figure 4. There was a small, transient decrease in respiratory rate: From its initial value of 14.1 breaths/min [11.3 to 16.9], it reached a nadir of 11.9 breaths/min [9.8 to 13.9] 1 min after remifentanil (P  < 0.05 vs.  baseline); by 2.5 min after injection, respiratory rate exceeded 13 breaths/min and no longer differed significantly from baseline.
Fig. 3. Time course of the computed ventilation at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 3. Time course of the computed ventilation at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P 
	< 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 3. Time course of the computed ventilation at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
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Fig. 4. Time course of the computed tidal volume at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 4. Time course of the computed tidal volume at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P 
	< 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 4. Time course of the computed tidal volume at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
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Comparison of figures 2 and 3revealed that the mean values of V̇E50 resulted in a much smoother curve than those of the carbon dioxide response slope. Therefore, we performed the pharmacodynamic analysis using V̇E50 to model the effect-site response. Table 1shows the mean of the subjects’ pharmacodynamic parameters (with confidence limits) and the pharmacodynamic parameters derived from the mean V̇E50 curve shown in figure 3. Figure 5Ashows how the mean values of V̇Ecompared with the plasma concentrations and pharmacodynamic effects predicted by the model, whereas figure 5Bshows the relation between computed effect-site concentration and the measured values of V̇E50 as well as those predicted by the sigmoidal model.
Table 1. Derived Pharmacodynamic Parameters for the Effect of a Single Dose of Remifentanil, 0.5 μg/kg Intravenous, on V̇E50.
Image not available
Table 1. Derived Pharmacodynamic Parameters for the Effect of a Single Dose of Remifentanil, 0.5 μg/kg Intravenous, on V̇E50.
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Fig. 5. (A  ) Time course of predicted plasma concentration (Cp), predicted pharmacodynamic effect, and the corresponding values of V̇E50 (measured effect) in the subjects. (B  ) Relation between effect-site concentration (from the pharmacokinetic–pharmacodynamic model) and the observed V̇E50 in the subjects. The dotted line is the sigmoidal function that predicts V̇E50 from the effect-site concentration.
Fig. 5. (A 
	) Time course of predicted plasma concentration (Cp), predicted pharmacodynamic effect, and the corresponding values of V̇E50 (measured effect) in the subjects. (B 
	) Relation between effect-site concentration (from the pharmacokinetic–pharmacodynamic model) and the observed V̇E50 in the subjects. The dotted line is the sigmoidal function that predicts V̇E50 from the effect-site concentration.
Fig. 5. (A  ) Time course of predicted plasma concentration (Cp), predicted pharmacodynamic effect, and the corresponding values of V̇E50 (measured effect) in the subjects. (B  ) Relation between effect-site concentration (from the pharmacokinetic–pharmacodynamic model) and the observed V̇E50 in the subjects. The dotted line is the sigmoidal function that predicts V̇E50 from the effect-site concentration.
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The time course of V̇E50 after remifentanil was closely approximated (r2= 0.99) by a biexponential function determined using nonlinear regression (fig. 6). On the basis of this regression, the T1/2for recovery from remifentanil-induced depression of V̇E50 was 6.2 min [4.3, 8.0].
Fig. 6. Nonlinear biexponential regression of V̇E50 versus  time, used to determine the offset T1/2for remifentanil. The measured values are shown to indicate the closeness of the exponential approximation.
Fig. 6. Nonlinear biexponential regression of V̇E50 versus 
	time, used to determine the offset T1/2for remifentanil. The measured values are shown to indicate the closeness of the exponential approximation.
Fig. 6. Nonlinear biexponential regression of V̇E50 versus  time, used to determine the offset T1/2for remifentanil. The measured values are shown to indicate the closeness of the exponential approximation.
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Discussion
Remifentanil is an ultra–short-acting opioid that relies on plasma esterases for rapid metabolism. Its short duration of action makes it an ideal agent to administer by continuous infusion. To most rapidly achieve steady state plasma concentrations, continuous infusions generally are initiated with a loading dose. With remifentanil, the recommended loading dose for monitored anesthesia care (0.5–1.0 μg/kg) can create a situation in which patients are apneic yet awake; they must be encouraged to breathe for a few minutes until PaCO2increases to the new apneic threshold.
To avoid this situation, the manufacturer (Glaxo Wellcome, Research Triangle Park, NC) recommends that the loading dose be administered over 30 s. Our data show that maximum ventilatory depression occurs approximately 2.5 min after a single loading dose is administered. This suggests that additional remifentanil administered within 2.5 min of the initial dose may produce further, unanticipated ventilatory depression, which is potentially dangerous in patients whose airways are not controlled. When the loading dose is followed by an infusion of remifentanil at 0.05 μg · kg−1· min−1, our pharmacodynamic model predicts that peak respiratory depression will occur approximately 5 min after the initial injection, and additional bolus doses of remifentanil during this time may be expected to exacerbate ventilatory depression.
By using spectral analysis of the EEG as a measure of the effect of remifentanil on the central nervous system, Egan et al.  observed an onset T1/2of 1.6 min. 3 This is approximately 60% of the T1/2that we observed for respiratory depression. This difference may be related in part to the fact that the surface EEG and ventilatory drive depend on different neural pathways; it also may be related to the finding that, in the previous investigation, much larger doses of remifentanil (30–60 μg/kg) were administered. Differing blood flows, blood–brain barrier characteristics, and neural responsiveness to opioids may contribute to a difference in the time necessary for pharmacodynamic effects to develop. The fact that the EC50for ventilatory depression, 1.1 ng/ml, was appreciably lower than the 19.9 ng/ml reported by Egan et al.  for EEG spectral edge depression 3 suggests that there are significant regional differences in opioid responsiveness within the central nervous system. This also corresponds to the clinical observation that patients may be apneic yet awake after intravenous administration of rapidly acting opioids.
Kapila et al.  characterized the pharmacodynamic effect of remifentanil by monitoring recovery of V̇Eat FETCO2= 7.5% after a 3-h infusion. 4 In that context, they found the offset T1/2to be 5.4 min. This is similar to the offset T1/2of 6.2 min observed in the current study, confirming that the recovery from the ventilatory effect of remifentanil essentially is independent of the duration of administration. Dershwitz et al.  also followed ventilatory drive by measuring V̇Eat FICO2= 7.5% before, during, and after a 4-h remifentanil infusion. 9 Based on their pooled data, these investigators found that EC50was 3.4 ng/ml in healthy subjects. Despite constant plasma concentrations, they observed a gradual decrease in respiratory depression during remifentanil infusion, suggesting that some degree of tolerance was developing. Acute tolerance may explain why the EC50for V̇Ethat we observed after a single dose of remifentanil was appreciably lower than the EC50observed after a 3- or 4-h infusion.
When remifentanil is used during general anesthesia with an established airway, ventilatory depression is not a major concern. However, when it is used as an analgesic in awake patients during monitored anesthesia care, the ventilatory depressant effects of remifentanil pose certain challenges. For example, ventilatory depression is not instantaneous; this mandates waiting a sufficient period of time (≈ 2.5 min) for the peak effect of first dose to develop before additional remifentanil is administered to avoid the risk of “stacking” doses and producing apnea. The finding that our subjects did not become apneic despite rapid administration of remifentanil suggests that stimulation of ventilation with a modest concentration (≈ 4%) of carbon dioxide might prevent apnea when remifentanil is administered for monitored anesthesia care.
The “dual isohypercapnic” method enabled us to determine ventilatory drive at 30-s intervals after remifentanil injection. Step-ramp rebreathing methods (e.g.  , Read rebreathing technique) 10 take several minutes to accomplish and necessitate a resting period between determinations, making them unsuitable for assessing rapid changes in ventilatory drive after a single dose of a short-acting drug. By measuring ventilatory drive in the 46- to 56-mmHg range, we reduced the likelihood of being on the “hockey stick” portion of the carbon dioxide response curve; this was confirmed by the finding that none of the subjects became apneic at either carbon dioxide tension after remifentanil.
A potential shortcoming of our study is that we did not measure plasma remifentanil levels directly; rather, we relied on previously published pharmacokinetic parameters to predict plasma concentrations in the subjects. Although there is significant interindividual variability in these parameters, the effect of this variability can be minimized by using pooled rather than individual data to perform the pharmacodynamic modeling. In fact, pharmacokinetic variability may help to explain the great variability in pharmacodynamic values that we observed when these were calculated using individual rather than pooled data.
In summary, we found that, in doses typically used during monitored anesthesia care, a single dose of remifentanil causes both a downward shift and a decrease in the slope of the ventilatory response to carbon dioxide. The onset T1/2for ventilatory depression was of longer duration than that reported for EEG depression, and peak ventilatory depression occurred approximately 2.5 min after remifentanil injection, suggesting that it is necessary to wait at least this long to assess ventilatory effects before administering additional medication. Recovery of ventilation was essentially complete within 15 min, consistent with the short elimination half-life of remifentanil. Differences between the EEG and ventilatory effects of remifentanil, as reflected in onset time and EC50help to explain the clinical observation that remifentanil may cause apnea in patients who are awake and responsive to verbal command.
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Westmoreland CL, Hoke JF, Sebel PS, Hug CC, Muir KT: Pharmacokinetics of remifentanil (GI87084B) and its major metabolite (GI90291) in patients undergoing elective inpatient surgery. A NESTHESIOLOGY 1993; 79:893–903Westmoreland, CL Hoke, JF Sebel, PS Hug, CC Muir, KT
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Dershwitz M, Hoke JF, Rosow CE, Michalowski P, Connors PM, Muir KT, Dienstag JL: Pharmacokinetics and pharmacodynamics of remifentanil in volunteer subjects with severe liver disease. A NESTHESIOLOGY 1996; 84:812–20Dershwitz, M Hoke, JF Rosow, CE Michalowski, P Connors, PM Muir, KT Dienstag, JL
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Fig. 1. Constructed carbon dioxide response curves before (time = 0) and at selected times after injection of 0.5 μg/kg remifentanil. These curves are based on mean values for V̇Eand PETCO2at each time point.
Fig. 1. Constructed carbon dioxide response curves before (time = 0) and at selected times after injection of 0.5 μg/kg remifentanil. These curves are based on mean values for V̇Eand PETCO2at each time point.
Fig. 1. Constructed carbon dioxide response curves before (time = 0) and at selected times after injection of 0.5 μg/kg remifentanil. These curves are based on mean values for V̇Eand PETCO2at each time point.
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Fig. 2. Time course of the slope of the ventilatory response to carbon dioxide after injection of remifentanil 0.5 μg/kg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 2. Time course of the slope of the ventilatory response to carbon dioxide after injection of remifentanil 0.5 μg/kg. Values below the dotted line differ significantly (P 
	< 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 2. Time course of the slope of the ventilatory response to carbon dioxide after injection of remifentanil 0.5 μg/kg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
×
Fig. 3. Time course of the computed ventilation at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 3. Time course of the computed ventilation at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P 
	< 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 3. Time course of the computed ventilation at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
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Fig. 4. Time course of the computed tidal volume at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 4. Time course of the computed tidal volume at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P 
	< 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
Fig. 4. Time course of the computed tidal volume at PETCO2= 50.0 mmHg. Values below the dotted line differ significantly (P  < 0.05) from baseline. Error bars indicate the upper 95% confidence limit.
×
Fig. 5. (A  ) Time course of predicted plasma concentration (Cp), predicted pharmacodynamic effect, and the corresponding values of V̇E50 (measured effect) in the subjects. (B  ) Relation between effect-site concentration (from the pharmacokinetic–pharmacodynamic model) and the observed V̇E50 in the subjects. The dotted line is the sigmoidal function that predicts V̇E50 from the effect-site concentration.
Fig. 5. (A 
	) Time course of predicted plasma concentration (Cp), predicted pharmacodynamic effect, and the corresponding values of V̇E50 (measured effect) in the subjects. (B 
	) Relation between effect-site concentration (from the pharmacokinetic–pharmacodynamic model) and the observed V̇E50 in the subjects. The dotted line is the sigmoidal function that predicts V̇E50 from the effect-site concentration.
Fig. 5. (A  ) Time course of predicted plasma concentration (Cp), predicted pharmacodynamic effect, and the corresponding values of V̇E50 (measured effect) in the subjects. (B  ) Relation between effect-site concentration (from the pharmacokinetic–pharmacodynamic model) and the observed V̇E50 in the subjects. The dotted line is the sigmoidal function that predicts V̇E50 from the effect-site concentration.
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Fig. 6. Nonlinear biexponential regression of V̇E50 versus  time, used to determine the offset T1/2for remifentanil. The measured values are shown to indicate the closeness of the exponential approximation.
Fig. 6. Nonlinear biexponential regression of V̇E50 versus 
	time, used to determine the offset T1/2for remifentanil. The measured values are shown to indicate the closeness of the exponential approximation.
Fig. 6. Nonlinear biexponential regression of V̇E50 versus  time, used to determine the offset T1/2for remifentanil. The measured values are shown to indicate the closeness of the exponential approximation.
×
Table 1. Derived Pharmacodynamic Parameters for the Effect of a Single Dose of Remifentanil, 0.5 μg/kg Intravenous, on V̇E50.
Image not available
Table 1. Derived Pharmacodynamic Parameters for the Effect of a Single Dose of Remifentanil, 0.5 μg/kg Intravenous, on V̇E50.
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