Free
Education  |   February 2002
Neuromuscular Junctions in Cerebral Palsy: Presence of Extrajunctional Acetylcholine Receptors
Author Affiliations & Notes
  • Mary C. Theroux, M.D.
    *
  • Robert E. Akins, Ph.D.
  • Carol Barone, H.T.-A.S.C.P.
  • Bobbie Boyce
    §
  • Freeman Miller, M.D.
  • Kirk W. Dabney, M.D.
  • * Clinical Associate Professor, Thomas Jefferson University, Philadelphia, Pennsylvania, and Department of Anesthesiology/Critical Care, Alfred I. duPont Hospital for Children. † Research Assistant Professor, Department of Pediatrics, Thomas Jefferson University, Philadelphia, Pennsylvania, and Department of Research, Alfred I. duPont Hospital for Children. ‡ Histotechnologist, § Research Technician, Department of Research, Alfred I. duPont Hospital for Children. ∥ Assistant Professor of Orthopedic Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, and Department of Orthopedics, Alfred I. duPont Hospital for Children.
  • Received from the Departments of Anesthesiology/Critical Care and Research, Alfred I. duPont Hospital for Children, Wilmington, Delaware.
Article Information
Education
Education   |   February 2002
Neuromuscular Junctions in Cerebral Palsy: Presence of Extrajunctional Acetylcholine Receptors
Anesthesiology 2 2002, Vol.96, 330-335. doi:
Anesthesiology 2 2002, Vol.96, 330-335. doi:
CEREBRAL palsy (CP) is a disorder of the central nervous system in which an insult sustained during fetal development or early postnatal life results in spasticity and motor dysfunction. The incidence rate for CP is approximately 0.7 per 1,000 live births. The prevalence of CP is increasing, 1 which is possibly related to the increasing number of infants who survive preterm delivery. Between 5 and 15% of roughly 40,000 preterm infants, weighing less than 1,500 g, develop major spastic motor deficits grouped under the rubric CP. Almost all patients with CP require surgical intervention and anesthesia from early life, so it is important to understand any aspects of CP that may affect the practice of anesthesia in these children.
An area for concern is the potential presence of abnormal neuromuscular junctions (NMJs) in patients with CP. Despite the high incidence of the disease and the involvement of the motor nervous system in the manifestation of the disease, the NMJs of children with CP have not been studied previously. 2 Based on indirect evidence obtained from studies of neuromuscular blocking agents, a possible up-regulation of nonjunctional acetylcholine receptors (AChRs) has been postulated in children with CP. 3,4 
We conducted the current study to directly examine and compare NMJs in biopsy samples from children with and without CP. Our approach was to perform in vitro  assessments on samples of erector spinae muscle taken during spinal fusion surgeries. Assessments included the determination of AChR subunit composition using molecular–biochemical assays and the detection of junctions by histologic staining. There were two null hypotheses in the study design: (1) there is no difference in the level of expression of γ-AChR subunits in muscles from children with CP when compared with muscles from children without CP; and (2) there is no difference between children with CP and normal children in the morphologic spread of AChRs in the NMJ.
Materials and Methods
Overall Design
The study design was prospective, nonrandomized, and partially blinded. Institutional review board approval was obtained for the study. Fetal tissue was used as control for reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting experiments. Per criteria established by the institutional review board, the fetal tissue was exempt from review as this tissue was from a preexisting sample without any patient identifiers.
Sixty-four children were enrolled in the study with parental consent. Thirty-nine children had stage 3 or 4 spastic quadriplegia, 5 and 25 children were neurologically normal. The staging of children with CP was accomplished using clinical criteria, which are scored on a 5-point scale: 0 = normal with no neuromotor defect; 1 = minor impairment with evidence of neuromotor involvement, such as increased tendon reflexes, but no functional impairment; 2 = mild disability with only minor loss of function such as abnormal gait; 3 = moderate disability with some purposeful voluntary movement possible; and 4 = severe disability that prevents virtually all purposeful voluntary movement. All children were undergoing spinal fusion for scoliosis.
Biopsy material was obtained from the paraspinal muscles at the thoracolumbar junction on the concave side of the curve, as far away from the spine as possible. This site was chosen because the concave side is thought to be more affected and the thoracolumbar junction provides a consistent and easily identified area in all patients, despite their scoliosis. Muscle biopsy specimens (approximately 1 cm3) were placed in sterile specimen containers and immersed in wet ice immediately after surgical excision. The tissue was trimmed and mounted for transverse sectioning on small cork-chucks using 8% tragacanth gum at the base. Mounted samples were snap-frozen in N2chilled isopentane for 30 s and stored at −70°C until needed. Sections (8 μm thick) of the frozen material were taken for use in the histologic and biochemical assays described below.
It should be noted that the choice of the erector spinae muscle for this study was based on our need to obtain control muscles from otherwise healthy, age-matched children. Spinal fusion for the correction of scoliosis was the only surgical procedure conducted with sufficient frequency to allow the procurement of muscle samples from identical sites for both study and control groups of children.
Exclusion Criteria
Patient samples that lacked innervation sites, as judged by exhaustive sectioning and histologic staining, were excluded from the study. A total of five patients were excluded from the entire study by this criterion.
Biochemical Determination of Acetylcholine Receptor Subunit Expression
Two methods were used for the biochemical detection of AChR subunits. RT-PCR was conducted to determine the concentration of mRNA present for both the α and γ subunits. Western blotting was conducted to determine the concentration of γ-subunit protein present.
Reverse-transcription Polymerase Chain Reaction.
Messenger RNA for the analysis of α- and γ-AChR subunit expression was evaluated by RT-PCR. Samples of RNA were collected from sections of frozen material using kits from Qiagen (Valencia, CA) and analyzed using standard RT-PCR procedures from Promega (Madison, WI). Primers (Cruachem, Dulles, VA) specific for α-AChR (forward: dTCATCAACACACACCACCGCTCAC; reverse: dCCATTGCAACGTACTTCCACTCTGC) and γ-AChR (forward: dAGCTGCTGAGGATGCACGTTC; reverse: dGCCTTTCTCTAGCTTCTCCAGC) resulted in the formation of a 331 base pair and a 199 base pair product, respectively, as verified by DNA sequence analysis. Single RT reactions were run, and the products were subjected to 30 cycles of PCR. The PCR products were visualized postelectrophoretically using ethidium bromide stain and an Eagle Eye Gel Documentation System (Stratagene, La Jolla, CA).
Western Blotting.
Sections of frozen material were collected and solubilized in sodium dodecyl sulfate polyacrylamide gel electrophoresis sample buffer containing Tris-HCl buffer, sodium dodecyl sulfate, glycerol, phenylmethylsulfonyl-fluoride, aminocaproic acid, benzamidene hydrochloride, and EDTA (all reagents from Sigma Chemical Co., St. Louis, MO). The samples were heated to 100°C for 2 min then centrifuged at 8,000 g  for 5 min to pellet any insoluble material. Samples were then partially purified using sequential ultrafiltration. Samples were diluted and subjected to ultrafiltration using a 30-kd cutoff Centricon-Plus-20®membrane (Fisher Scientific, Pittsburgh, PA). The retentate was recovered and passed through a 100-kd Microcon®(Fisher) filtration membrane, which was subsequently rinsed. The filtrate and rinse were combined then collected using a 30-kd cutoff Microcon®(Fisher) filtration membrane. In this way, proteins between 30 and 100 kd were partially purified. Samples were then resuspended in sodium dodecyl sulfate polyacrylamide gel electrophoresis sample buffer with β mercaptoethanol added and reheated to 100°C. The concentration of protein was determined using a Bicinchoninic-based assay (Pierce Chemical Co., Rockford, IL), and 50 μg of each sample was loaded onto polyacrylamide gels (BioRad Laboratories, Hercules, CA). The proteins were electrophoretically separated and transferred to polyvinylidene-difluoride (BioRad) membranes using Mini-Protean II and Mini-Blot equipment (BioRad). The membranes were blocked with 3% bovine serum albumin in phosphate-buffered saline, and bands were detected by enhanced chemiluminescence (BioRad) using an antibody specific for γ-AChR (Santa Cruz Biotechnology, Santa Cruz, CA).
Histologic Evaluation of Neuromuscular Junctions
To evaluate the presence of AChRs outside of NMJs, a double-stain method was developed to examine both AChR and acetylcholinesterase. In the past, researchers relied on measuring the spread of AChR staining patterns alone. 2 In the current methodology, a second marker, namely, immunoreactive acetylcholinesterase, was used to indicate the limits of the NMJ. Acetylcholinesterase was chosen because of its well-characterized expression pattern 6,7 and its localization to the functional NMJ. 8 An antibody specific for acetylcholinesterase was used with a fluorescent secondary antibody to stain sections. In conjunction with the acetylcholinesterase detection, AChRs were stained using α bungarotoxin (α-BTX) conjugated with a second fluorophore. In this way, the limits of the NMJ, as defined by distinct acetylcholinesterase staining, and the distribution of AChR could be evaluated separately or simultaneously within a single section of biopsy material. Typically, the acetylcholinesterase was stained green using a fluorescein-conjugated secondary antibody, and the AChR was stained red using rhodamine-conjugated α-BTX. The addition of red and green fluorescence signals during simultaneous viewing of the fluorophores resulted in a yellow coloration where both molecules were present.
For microscopic localization of AChR, slides containing biopsy cryosections were fixed at room temperature for 25 min using neutral-buffered formalin. These were rinsed with phosphate-buffered saline and stained in a solution containing 2 μg/ml tetramethylrhodamine-conjugated α-BTX (Molecular Probes, Inc., Eugene, OR). After 1-h incubation at room temperature, the sections were rinsed with phosphate-buffered saline and restained using an antibody to acetylcholinesterase (AE-2; Biogenesis, Inc., Sansdown, NH) diluted 1:100 in phosphate-buffered saline and a fluorescein-conjugated secondary antibody (Jackson Labs, West Chester, PA). Samples were viewed and photographed on an Olympus BX-60 microscope (Olympus, Tokyo, Japan). The limits of the NMJ were defined as the extent of the acetylcholinesterase staining (which appeared green in the microscope), and samples were scored positive for abnormal presence of AChRs if α-BTX staining (which appeared red in the microscope) extended beyond this acetylcholinesterase-defined limit. When the acetylcholinesterase and AChR distributions were viewed simultaneously, areas of overlap appeared yellow. One investigator prepared photographs of each NMJ that was observed. Two blinded investigators evaluated the photographs for the presence or absence of abnormal AChRs. In this study, punctate signals were ignored as possible artifact or nonspecific staining. A patient was scored positive for the presence of abnormal AChRs when both scorers agreed that extrajunctional staining was present.
Statistical Analysis
Based on a pilot study of 10 patients, 40% of CP patients and 0% of non-CP patients were expected to have abnormal AChRs. Using a conservative estimate of 30% positives expected in the CP group, to achieve a power equal to 0.8, a sample size of 20 patients per group was determined. The presence or absence of abnormal AChRs, determined by histologic staining, was analyzed using chi-square analysis. The presence or absence of AChR subunits by RT-PCR or Western blotting was not analyzed because of the absence of detectable signal.
Results
Patient Demographics
The group undergoing spinal fusion surgery for idiopathic scoliosis (non-CP group) had a mean age of 14.2 yr (range, 7.8–15 yr) and a mean weight of 56.7 kg (range, 35–107 kg). The group undergoing spinal fusion surgery for scoliosis associated with CP (CP group) had a mean age of 11.5 yr (range, 7–15 yr) and a mean weight of 24.5 kg (range, 13–55 kg). All but eight patients in the CP group were receiving anticonvulsants. The most commonly used anticonvulsant was valproic acid, followed by phenobarbital, clonazepam, carbamazepine, primidone, and lamotrigine. None of the children with idiopathis scoliosis were receiving anticonvulsants.
Histologic Staining of Neuromuscular Junctions
Figure 1shows representative staining patterns from NMJs of children with idiopathic scoliosis. These NMJs were all judged to be negative for abnormal presence of AChRs. Column A is the acetylcholinesterase distribution shown in green. Column B shows the AChR distribution in red fluorescence. Column C shows both red and green fluorescence signals visualized together; where the two distributions overlap, a yellowish color was seen. In all cases, the NMJs of children with idiopathic scoliosis appeared normal. In the samples from the 20 patients with idiopathic scoliosis, no AChRs were found outside the NMJ.
Fig. 1. Histologic examination of neuromuscular junctions (NMJs) from children with idiopathic scoliosis. NMJs from seven children are shown. These represent typical staining patterns for NMJs in children with a diagnosis of idiopathic scoliosis. Column A shows the staining pattern for acetylcholine esterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. In all cases, the NMJs in these children showed AChR staining that was contained within the limits of the acetylcholinesterase staining.
Fig. 1. Histologic examination of neuromuscular junctions (NMJs) from children with idiopathic scoliosis. NMJs from seven children are shown. These represent typical staining patterns for NMJs in children with a diagnosis of idiopathic scoliosis. Column A shows the staining pattern for acetylcholine esterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. In all cases, the NMJs in these children showed AChR staining that was contained within the limits of the acetylcholinesterase staining.
Fig. 1. Histologic examination of neuromuscular junctions (NMJs) from children with idiopathic scoliosis. NMJs from seven children are shown. These represent typical staining patterns for NMJs in children with a diagnosis of idiopathic scoliosis. Column A shows the staining pattern for acetylcholine esterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. In all cases, the NMJs in these children showed AChR staining that was contained within the limits of the acetylcholinesterase staining.
×
Figure 2shows representative staining patterns from NMJs of children with CP. These NMJs were all judged to be positive for abnormal presence of AChRs. Similar to figure 1, column A shows the limits of the NMJ as indicated by acetylcholinesterase, column B shows the distribution of AChRs, and column C shows both signals together. After all three panels were viewed, the presence of abnormal AChRs was judged. The arrows in column C indicate the location of AChRs outside of the NMJ, based on red staining extending beyond the acetylcholinesterase-defined NMJ. In 11 of the 39 samples from patients with CP, abnormal spread of AChRs was found. Chi-square analysis indicated a significant difference between the CP group and the non-CP group (P  = 0.0085).
Fig. 2. Histologic examination of neuromuscular junctions (NMJs) from children with cerebral palsy (CP). Neuormuscular junctions from seven children with a diagnosis of scoliosis associated with CP are shown. Column A shows the staining pattern for acetylcholinesterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. Each of the seven NMJs shown was scored positive for the presence of extrajunctional AChR receptors.
Fig. 2. Histologic examination of neuromuscular junctions (NMJs) from children with cerebral palsy (CP). Neuormuscular junctions from seven children with a diagnosis of scoliosis associated with CP are shown. Column A shows the staining pattern for acetylcholinesterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. Each of the seven NMJs shown was scored positive for the presence of extrajunctional AChR receptors.
Fig. 2. Histologic examination of neuromuscular junctions (NMJs) from children with cerebral palsy (CP). Neuormuscular junctions from seven children with a diagnosis of scoliosis associated with CP are shown. Column A shows the staining pattern for acetylcholinesterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. Each of the seven NMJs shown was scored positive for the presence of extrajunctional AChR receptors.
×
Among the 11 patients with CP who scored positive for the presence of AChRs outside of the NMJ, six were receiving anticonvulsants. Conversely, of the 28 patients with CP who scored negative for the presence of AChRs outside of the NMJ, 25 were receiving anticonvulsants.
Expression of γ-Acetylcholine Receptor Subunits
We used two methods to look for expression of the γ subunit in these samples. RT-PCR using primers specific for the α and γ subunits was performed, and the results are summarized in figure 3A. The mRNA for α was present in each sample; however, the expression of the mRNA for γ was unexpectedly absent from all samples. These results suggested that AChRs containing the γ subunit were not present as a result of CP. Because little is known about the turnover of γ-AChR mRNA, Western blotting was also performed in an attempt to detect this subunit. Similar to the RT-PCR results, we were unable to detect γ-AChR in any of the samples from CP or non-CP patients. Figure 3Bshows a representative Western blot analysis. These RT-PCR and Western blotting results suggest that the abnormal spread of AChRs found histologically in children with CP may differ from those found during development or postdenervation in terms of subunit composition.
Fig. 3. (A  ) Representative reverse-transcription polymerase chain reaction analysis of acetylcholine receptor (AChR) subunits. Samples were assessed for the presence of the α- and γ-AChR subunits. Lane 1: positive control for both α- and γ-AChR prepared from fetal tissue. Lanes 2–5: samples from four children with idiopathic scoliosis. Lanes 6–9: samples from four children with cerebral palsy. All samples were positive for the α subunit and negative for the γ subunit. (B  ) Representative Western blot showing the absence of detectable staining for the γ-AChR subunit protein. Lane 1: positive control for γ-AChR. Lanes 2–5: samples from four children with a diagnosis of idiopathic scoliosis. Lanes 6–9: samples from four children with a diagnosis of scoliosis associated with cerebral palsy and a positive score for the abnormal spread of AChRs by histologic assessment. These results suggest that the γ subunit of the AChR is not being expressed in the erector spinae muscle of children with cerebral palsy.
Fig. 3. (A 
	) Representative reverse-transcription polymerase chain reaction analysis of acetylcholine receptor (AChR) subunits. Samples were assessed for the presence of the α- and γ-AChR subunits. Lane 1: positive control for both α- and γ-AChR prepared from fetal tissue. Lanes 2–5: samples from four children with idiopathic scoliosis. Lanes 6–9: samples from four children with cerebral palsy. All samples were positive for the α subunit and negative for the γ subunit. (B 
	) Representative Western blot showing the absence of detectable staining for the γ-AChR subunit protein. Lane 1: positive control for γ-AChR. Lanes 2–5: samples from four children with a diagnosis of idiopathic scoliosis. Lanes 6–9: samples from four children with a diagnosis of scoliosis associated with cerebral palsy and a positive score for the abnormal spread of AChRs by histologic assessment. These results suggest that the γ subunit of the AChR is not being expressed in the erector spinae muscle of children with cerebral palsy.
Fig. 3. (A  ) Representative reverse-transcription polymerase chain reaction analysis of acetylcholine receptor (AChR) subunits. Samples were assessed for the presence of the α- and γ-AChR subunits. Lane 1: positive control for both α- and γ-AChR prepared from fetal tissue. Lanes 2–5: samples from four children with idiopathic scoliosis. Lanes 6–9: samples from four children with cerebral palsy. All samples were positive for the α subunit and negative for the γ subunit. (B  ) Representative Western blot showing the absence of detectable staining for the γ-AChR subunit protein. Lane 1: positive control for γ-AChR. Lanes 2–5: samples from four children with a diagnosis of idiopathic scoliosis. Lanes 6–9: samples from four children with a diagnosis of scoliosis associated with cerebral palsy and a positive score for the abnormal spread of AChRs by histologic assessment. These results suggest that the γ subunit of the AChR is not being expressed in the erector spinae muscle of children with cerebral palsy.
×
Discussion
Denervation and immobilization are typical conditions in which AChRs are found outside the NMJ 9,10 Neurologic diseases, such as amyotrophic lateral sclerosis, peripheral neuropathy, and infantile spinal muscular atrophy, show the morphologic and histochemical changes of denervation, consisting of scattered and grouped small fibers with variable muscle fiber–type groupings. α-BTX–linked immunoperoxidase staining has indicated that in many of the muscle fibers in these disorders, AChRs are spread over the surface muscle fibers. 2 These diffusely spread AChRs have been linked to the hyperkalemia seen when these patients are exposed to succinylcholine. 10–12 
The γ subunit of the AChR, which was expected to be found in cases where nonjunctional AChRs were present, was not detected by RT-PCR in the current study. This was true even in patients who exhibited extrajunctional AChRs by histologic assessment. Because the turnover of AChR mRNA may be rapid, we attempted to verify or refute the RT-PCR results by Western blotting and obtained similar results. Based on our observations, we concluded that some children with CP possess extrajunctional AChRs but do not express the γ subunit; however, it is also possible that the mRNA for γ-AChR is significantly less stable than the mRNA for the α subunit and that the amount of γ-AChR protein in our samples is below the detection limit of the Western approach.
The protein subunit composition of skeletal muscle AChRs changes both during the course of development and in response to denervation of mature muscle. 13–16 During certain pathologic conditions, changes in the distribution of AChRs have been reported. The most common such pathologic state is denervation 17 in which there is an up-regulation of the γ-AChR subunit mediated by transcriptional activation. Another classic case in which AChR concentrations are increased is in burn victims, who show a nontranscriptionally mediated up-regulation. 18 The latter study by Nosek and Martyn 18 demonstrated a quantitative increase in AChR without expression of γ subunit. Our study demonstrates a spread of receptor beyond the junctional area without expression of γ subunit.
Clinical experience supports the notion that children with CP have extrajunctional AChRs. Children with CP have a slightly increased sensitivity to the depolarizing agent succinylcholine. The effective dose for 50% twitch depression was lower in children with spastic quadriplegic CP than for age-matched normal children. 3 Similarly, the duration of action of vecuronium, a nondepolarizing muscle relaxant, was significantly decreased 4 in children with CP when compared with children without CP. However, in this study, children with CP had also been receiving anticonvulsant therapy. The known interaction and up-regulation of AChRs when exposed to anticonvulsants 19–22 may have contributed to the vecuronium resistance. Interestingly, in our study, only 6 of the 11 children who showed histologic evidence of AChRs outside the functional NMJ were on anticonvulsants, whereas 5 of 11 children who showed histologic evidence of AChRs were not.
Other studies have demonstrated an increased requirement for nondepolarizing muscle relaxant with quantitative increase in AChR. Chronic infusion of d-tubocurarine results in proliferation of AChRs and increased requirement for d-tubocurarine. 23 This study points to the phenomenon of up-regulation of AChRs by long-term use of an agent that is an inhibitor of AChRs. There are clinical studies 3,4 pointing toward up-regulation of AChRs in CP, although the cause of this up-regulation remains unclear.
In summary, our findings of an abnormal spread of AChRs in children with CP are consistent with clinical experience and previous studies. The γ subunit was not detected either by RT-PCR or by Western blotting experiments even in children who had histologic evidence of nonjunctional AChRs. This observation is consistent with observation by Nosek and Martyn, 18 who detected a quantitative increase in AChR in a burn animal model without detecting γ subunit. These observations differ from results seen in other studies of changes after denervation 24–26 in which expression of the γ subunit correlates with both an altered distribution and an up-regulation of AChRs . 
In conclusion, children with spastic CP had histologic evidence of spread of AChRs beyond the confines of the functional NMJ when compared with children without CP. On further examination of the subunits by RT-PCR and Western blotting, no γ subunit was detected. These findings (spread of AChRs beyond the NMJs) are consistent with previous clinical findings in children with CP.
The authors thank Mary Carango, B.S., Terry McLaughlin, David DuHadaway, B.S., and Kara Gratton, M.S. (all at the Department of Biomedical Research, Alfred I. duPont Hospital for Children, Wilmington, DE), for expert technical assistance.
References
Volpe JJ: Brain injury in the premature infant: Current concepts. Prevent Med 1994; 23: 638–45Volpe, JJ
Bender AN, Ringel SP, Engel WK: The acetylcholine receptor in normal and pathologic states: Immunoperoxidase visualization of alpha-bungarotoxin binding at a light and electron-microscope level. Neurology 1976; 26: 477–83Bender, AN Ringel, SP Engel, WK
Theroux MC, Brandom BW, Zagnoev M, Kettrick RG, Miller F, Ponce C: Dose response of succinylcholine at the adductor pollicis of children with cerebral palsy during propofol and nitrous oxide anesthesia. Anesth Analg 1994; 79: 761–5Theroux, MC Brandom, BW Zagnoev, M Kettrick, RG Miller, F Ponce, C
Moorthy SS, Krishna G, Dierdorf SF: Resistence to vecuronium in patients with cerebral palsy. Anesth Analg 1991; 73: 275–7Moorthy, SS Krishna, G Dierdorf, SF
Evans PM, Alberman E: Recording motor defects of children with cerebral palsy (letter). Dev Med Child Neurol 1985; 27: 404–6Evans, PM Alberman, E
Rubin LL, Schuetz SM, Weil CL, Fischbach GD: Regulation of acetylcholinesterase appearance at neuromuscular junctions in vitro. Nature 1980; 283: 262–7Rubin, LL Schuetz, SM Weil, CL Fischbach, GD
Lomo T, Slater CR: Control of junctional acetylcholinesterase by neural and muscular influences in the rat. J Physiol 1980; 303: 191–202Lomo, T Slater, CR
Welchum L, Burgess RW, Dominguez B, Pfaff SL, Sanes JR, Lee KF: Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse. Nature 2001; 410: 1057–64Welchum, L Burgess, RW Dominguez, B Pfaff, SL Sanes, JR Lee, KF
Fambrough DM: Control of acetylcholine receptors in skeletal muscle. Physiol Rev 1979; 59: 165–227Fambrough, DM
Martyn JA, White DA, Gronert GA, Jaffe RS, Ward JM: Up and down regulation of skeletal muscle acetylcholine receptors: Effects on neuromuscular blockers. A nesthesiology 1992; 76: 822–43Martyn, JA White, DA Gronert, GA Jaffe, RS Ward, JM
Gronert GA, Theye RA: Pathophysiology of hyperkalemia induced by succinylcholine. A nesthesiology 1975; 43: 89–99Gronert, GA Theye, RA
Tobey RE, Jacobsen PM, Kahle CT, Clubb RJ, Dean MA: The serum potassium response to muscle relaxants in neural injury. A nesthesiology 1972; 37: 332–7Tobey, RE Jacobsen, PM Kahle, CT Clubb, RJ Dean, MA
Loring RH, Salpeter MM: Denervation increases turnover rate of junctional acetylcholine receptors. Proc Natl Acad Sci U S A 1980; 77: 2293–7Loring, RH Salpeter, MM
Tsay HJ, Schmidt J: Skeletal muscle denervation activates acetylcholine receptor genes. J Cell Biol 1989; 108: 1523–6Tsay, HJ Schmidt, J
Bevan S, Steinbach JH: The distribution of alpha bungarotoxin binding sites of mammalian skeletal muscle developing in vivo. J Physiol 1977; 267: 195–213Bevan, S Steinbach, JH
Burden S: Acetylcholine receptors at the neuromuscular junction: Developmental change in receptor turnover. Dev Biol 1977; 61: 79–85Burden, S
Wtzemann V, Brenner HR, Sakmann B: Neural factors regulate AchR subunit mRNA at rat neuromuscular synapses. J Cell Biol 1991; 114: 125–41Wtzemann, V Brenner, HR Sakmann, B
Nosek MT, Martyn JA: Na+ channel and acetylcholine receptor changes in muscle at sites distant from burns do not simulate denervation. J Appl Physiol 1997; 82: 1333–9Nosek, MT Martyn, JA
Melton AT, Antognini JF, Gronert GA: Prolonged duration of succinylcholine in patients receiving anticonvulsants: Evidence for mild up-regulation of acetylcholine receptors? Can J Anaesth 1993; 40: 939–42Melton, AT Antognini, JF Gronert, GA
Ornstein E, Matteo RS, Schwartz AE, Silverberg PA, Young WL, Diaz J: The effect of phenytoin on the magnitude and duration of neuromuscular block following atracurium or vecuronium. A nesthesiology 1987; 67: 191–6Ornstein, E Matteo, RS Schwartz, AE Silverberg, PA Young, WL Diaz, J
Yaari Y, Pincus JH, Argov Z: Depression of synaptic transmission by diphenylhydantoin. Ann Neurol 1977; 1: 334–8Yaari, Y Pincus, JH Argov, Z
Roth S, Ebrahim ZY: Resistence to pancuronium in patients receiving carbamazepine. A nesthesiology 1987; 66: 691–3Roth, S Ebrahim, ZY
Hogue CW, Ward JM, Itani MS, Martyn AJ: Tolerance and upregulation of acetylcholine receptors follow chronic infusion of d-tubocurarine. J Appl Physiol 1992; 72: 1326–31Hogue, CW Ward, JM Itani, MS Martyn, AJ
Goldman D, Brenner HR, Heinemann S: Acetylcholine receptor alpha-, beta-, gamma-, and delta-subunit mRNA levels are regulated by muscle activity. Neuron 1988; 1: 329–33Goldman, D Brenner, HR Heinemann, S
Shuetze SM, Role LW: Developmental regulations of nicotinic acetylcholine receptors. Ann Rev Neurosci 1987; 10: 403–57Shuetze, SM Role, LW
Lukas RJ, Bencherif M: Heterogeneity and regulation of nicotinic acetylcholine receptors. Int Rev Neurobiol 1992; 34: 25–31Lukas, RJ Bencherif, M
Fig. 1. Histologic examination of neuromuscular junctions (NMJs) from children with idiopathic scoliosis. NMJs from seven children are shown. These represent typical staining patterns for NMJs in children with a diagnosis of idiopathic scoliosis. Column A shows the staining pattern for acetylcholine esterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. In all cases, the NMJs in these children showed AChR staining that was contained within the limits of the acetylcholinesterase staining.
Fig. 1. Histologic examination of neuromuscular junctions (NMJs) from children with idiopathic scoliosis. NMJs from seven children are shown. These represent typical staining patterns for NMJs in children with a diagnosis of idiopathic scoliosis. Column A shows the staining pattern for acetylcholine esterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. In all cases, the NMJs in these children showed AChR staining that was contained within the limits of the acetylcholinesterase staining.
Fig. 1. Histologic examination of neuromuscular junctions (NMJs) from children with idiopathic scoliosis. NMJs from seven children are shown. These represent typical staining patterns for NMJs in children with a diagnosis of idiopathic scoliosis. Column A shows the staining pattern for acetylcholine esterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. In all cases, the NMJs in these children showed AChR staining that was contained within the limits of the acetylcholinesterase staining.
×
Fig. 2. Histologic examination of neuromuscular junctions (NMJs) from children with cerebral palsy (CP). Neuormuscular junctions from seven children with a diagnosis of scoliosis associated with CP are shown. Column A shows the staining pattern for acetylcholinesterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. Each of the seven NMJs shown was scored positive for the presence of extrajunctional AChR receptors.
Fig. 2. Histologic examination of neuromuscular junctions (NMJs) from children with cerebral palsy (CP). Neuormuscular junctions from seven children with a diagnosis of scoliosis associated with CP are shown. Column A shows the staining pattern for acetylcholinesterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. Each of the seven NMJs shown was scored positive for the presence of extrajunctional AChR receptors.
Fig. 2. Histologic examination of neuromuscular junctions (NMJs) from children with cerebral palsy (CP). Neuormuscular junctions from seven children with a diagnosis of scoliosis associated with CP are shown. Column A shows the staining pattern for acetylcholinesterase (AChEase), column B shows the staining pattern for acetylcholine receptor (AChR), and column C shows the combined fluorescence images. Each of the seven NMJs shown was scored positive for the presence of extrajunctional AChR receptors.
×
Fig. 3. (A  ) Representative reverse-transcription polymerase chain reaction analysis of acetylcholine receptor (AChR) subunits. Samples were assessed for the presence of the α- and γ-AChR subunits. Lane 1: positive control for both α- and γ-AChR prepared from fetal tissue. Lanes 2–5: samples from four children with idiopathic scoliosis. Lanes 6–9: samples from four children with cerebral palsy. All samples were positive for the α subunit and negative for the γ subunit. (B  ) Representative Western blot showing the absence of detectable staining for the γ-AChR subunit protein. Lane 1: positive control for γ-AChR. Lanes 2–5: samples from four children with a diagnosis of idiopathic scoliosis. Lanes 6–9: samples from four children with a diagnosis of scoliosis associated with cerebral palsy and a positive score for the abnormal spread of AChRs by histologic assessment. These results suggest that the γ subunit of the AChR is not being expressed in the erector spinae muscle of children with cerebral palsy.
Fig. 3. (A 
	) Representative reverse-transcription polymerase chain reaction analysis of acetylcholine receptor (AChR) subunits. Samples were assessed for the presence of the α- and γ-AChR subunits. Lane 1: positive control for both α- and γ-AChR prepared from fetal tissue. Lanes 2–5: samples from four children with idiopathic scoliosis. Lanes 6–9: samples from four children with cerebral palsy. All samples were positive for the α subunit and negative for the γ subunit. (B 
	) Representative Western blot showing the absence of detectable staining for the γ-AChR subunit protein. Lane 1: positive control for γ-AChR. Lanes 2–5: samples from four children with a diagnosis of idiopathic scoliosis. Lanes 6–9: samples from four children with a diagnosis of scoliosis associated with cerebral palsy and a positive score for the abnormal spread of AChRs by histologic assessment. These results suggest that the γ subunit of the AChR is not being expressed in the erector spinae muscle of children with cerebral palsy.
Fig. 3. (A  ) Representative reverse-transcription polymerase chain reaction analysis of acetylcholine receptor (AChR) subunits. Samples were assessed for the presence of the α- and γ-AChR subunits. Lane 1: positive control for both α- and γ-AChR prepared from fetal tissue. Lanes 2–5: samples from four children with idiopathic scoliosis. Lanes 6–9: samples from four children with cerebral palsy. All samples were positive for the α subunit and negative for the γ subunit. (B  ) Representative Western blot showing the absence of detectable staining for the γ-AChR subunit protein. Lane 1: positive control for γ-AChR. Lanes 2–5: samples from four children with a diagnosis of idiopathic scoliosis. Lanes 6–9: samples from four children with a diagnosis of scoliosis associated with cerebral palsy and a positive score for the abnormal spread of AChRs by histologic assessment. These results suggest that the γ subunit of the AChR is not being expressed in the erector spinae muscle of children with cerebral palsy.
×