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Pain Medicine  |   February 2003
Isoflurane Decreases ATP Sensitivity of Guinea Pig Cardiac Sarcolemmal KATPChannel at Reduced Intracellular pH
Author Affiliations & Notes
  • Anna Stadnicka, Ph.D.
    *
  • Zeljko J. Bosnjak, Ph.D.
  • * Assistant Professor of Anesthesiology, † Professor of Anesthesiology and Physiology.
  • Received from the Department of Anesthesiology, the Medical College of Wisconsin, Milwaukee, Wisconsin.
Article Information
Pain Medicine
Pain Medicine   |   February 2003
Isoflurane Decreases ATP Sensitivity of Guinea Pig Cardiac Sarcolemmal KATPChannel at Reduced Intracellular pH
Anesthesiology 2 2003, Vol.98, 396-403. doi:0000542-200302000-00020
Anesthesiology 2 2003, Vol.98, 396-403. doi:0000542-200302000-00020
THE adenosine triphosphate (ATP)-sensitive potassium (KATP) channels are thought to play an important role in anesthetic preconditioning, a protection afforded by volatile anesthetics against ischemia and reperfusion injury. 1–6 Although cellular mechanisms of this protection remain the focus of many investigations, direct evidence for interaction of volatile anesthetics with the KATPchannel is still limited. 7,8 Such an interaction is clinically important because volatile anesthetics may mimic or enhance the protective mechanism of KATPchannel opening.
A characteristic property of KATPchannels is their sensitivity to inhibition by physiologic concentrations of intracellular ATP ([ATP]i) that is controlled by a number of cytosolic factors including nucleotide diphosphates (ADP), phospholipids such as phosphatidylinositol 4,5-biphosphate (PIP2), and intracellular protons. Intracellular pH (pHi) modulates the activity of native KATPchannels in pancreatic β cells, 9 skeletal muscle, 10,11 heart, 12–16 and cloned KATPchannels in Xenopus  oocyte expression system. 17–19 The acidic species of KATPchannel modulators have been implicated in the mechanism of pH-dependent regulation of sensitivity to ATP. 20 An interaction of intracellular protons with ATP in regulating channel activity has been also suggested recently. 21 High sensitivity to activation by intracellular protons implies an important role for KATPchannels in regulation of cellular excitability during various metabolic stresses that often are accompanied by a decrease in pHi. However, whether pHi is a factor in volatile anesthetic–KATPchannel interaction has not been established.
We have recently reported that during whole cell or cell-attached patch clamp conditions isoflurane potentiates the cardiac KATPchannel current (IKATP) and increases open probability (Po) of channels previously activated by an uncoupler of oxidative phosphorylation, 2,4-dinitrophenol, and the KATPchannel opener, pinacidil. 8,22 Potentiation, however, did not occur in the inside-out patches where at pHi 7.4 isoflurane either had no effect or decreased channel activity. 7,8 Differential on-cell versus  cell-free effects of isoflurane suggested that other intracellular factors might be involved in anesthetic potentiation.
In the present study, we tested the hypothesis that pHi is one of the endogenous factors modulating isoflurane interaction with the cardiac KATPchannel. Because intracellular acidosis, an effect characteristic of early ischemia, may also occur perioperatively before or during administration of the volatile anesthetic agents, we investigated whether decreasing pHi alters interaction of isoflurane with the KATPchannel regarding modulation of channel Po and sensitivity to inhibition by [ATP]i.
Materials and Methods
Cell Isolation
After approval by the Institutional Animal Use and Care Committee of the Medical College of Wisconsin, single ventricular myocytes were isolated from guinea pig hearts by enzymatic dissociation with collagenase Type II, (Gibco/Invitrogen, Life Technologies, Grand Island, NY) and protease Type XIV, (Sigma, St Louis, MO) as reported previously. 23 Ventricular myocytes were stored in modified Tyrode solution, and only calcium-tolerant, rod-shaped cells with distinct cross-striations were used for experiments within 8 h after isolation.
Solutions
The modified Tyrode solution contained NaCl, 132 mm; KCl, 4.8 mm; MgCl2, 1.2 mm; CaCl2, 1 mm; HEPES, 10 mm; and glucose, 5 mm; at pH adjusted to 7.4 with NaOH.
For single channel recordings in the inside-out patch clamp configuration, the bath solution facing the intracellular side of membrane patches contained KCl, 140 mm; MgCl2, 0.5 mm; EGTA, 1 mm; HEPES, 10 mm; and variable 0–1 mm K2-ATP, at pH 7.4 or pH 6.8 adjusted with KOH or HCl. The pipette solution facing the extracellular side of membrane patches contained KCl, 140 mm; MgCl2, 0.5 mm; CaCl2, 0.5 mm; and HEPES, 10 mm at pH 7.4 adjusted with KOH. All chemicals were purchased from Sigma (St. Louis, MO).
Isoflurane (Baxter Healthcare, Deerfield, IL) was delivered to the recording chamber in the bath solution. Anesthetic solution was prepared by adding a measured aliquot of isoflurane to a known volume of bath solution and dispersing it by sonication. This solution was then transferred into a gas-tight glass syringe reservoir to be delivered to the recording chamber by a gravity-fed perfusion system. Isoflurane was used at a clinically relevant concentration of 0.53 ± 0.04 mm (n = 94) equivalent to 1.06 vol% at 20–23°C, or 0.9 minimum alveolar concentration (MAC) in guinea pigs and humans. This concentration was chosen because at 1.0 MAC, isoflurane has been shown to protect the myocardium in vivo  and in vitro  3,6 and to enhance activity of whole cell IKATPand single KATPchannels. 8,22 Isoflurane concentrations were determined by the headspace analysis method using a Shimadzu GC 8A flame ionization detection gas chromatograph (Shimadzu, Kyoto, Japan) from aliquots of the bath solution sampled directly from the recording chamber.
Single Channel Recordings and Analysis
Ventricular cells were placed in a RC-16 recording chamber (Warner, Hamden, CT) on the stage of an inverted IMT-2 microscope (Olympus, Tokyo, Japan). Single KATPchannel activity was monitored in the inside-out configuration of the patch clamp technique at 20–23°C. Patch pipettes were pulled from borosilicate glass tubing (Garner Glass, Claremont, CA) with a horizontal PC-84 micropipette puller (Sutter Instruments, Novato, CA). The tips were heat-polished with an MF-83 microforge (Narishige, Tokyo, Japan). Pipettes had resistances of 7–12 MΩ when filled with the extracellular solution. After gigaseal formation, the inside-out patches were excised by rapidly pulling the pipette away from the cell. In this configuration, the intracellular side of the membrane patch was directly exposed to the intracellular bath solution. Channel activity was recorded using a List EPC-7 amplifier (ALA Scientific Instruments, Westbury, NY) interfaced to a personal computer through a Digiata 1200B (Axon Instruments, Foster City, CA). Data were acquired using pClamp8 software (Axon Instruments, Foster City, CA). The current signal was filtered at 500 Hz through an 8-pole Bessel low-pass filter and sampled at 1 kHz. Single channel data were analyzed with pClamp8 software (Axon Instruments, Foster City, CA) and Origin6 software (OriginLab, Northampton, MA).
At symmetric 140 mm K+concentration, unitary outward current through single KATPchannels was monitored at the transmembrane patch potential of +40 mV. The 60-s recordings were made at each experimental step. The KATPchannels were identified by the single channel conductance, sensitivity to inhibition by [ATP]i, and blockade by glibenclamide (1 μm). A 50% threshold criterion was used for detecting the open state. Amplitude of single channel current was determined from the all-points amplitude histograms constructed from data segments of 60-s duration. Channel Po was determined from the ratios of the area under the peaks in the all-points amplitude histograms fitted with a Gaussian function. The number (N) of channels in each patch was estimated during brief exposure to ATP-free internal solution (0 ATP) at the end of the experiments. Po was calculated using the equation Po =[1 − (Pc)1/n] where Pc is the channel closed state probability. For measurements of ATP sensitivity, Po of each patch was normalized to Po determined at 0 ATP to control for variations in Po among patches. The sensitivity to ATP (1, 10, 50, 100, and 1,000 μm) was determined at pHi 7.4 and pHi 6.8 in the absence or presence of isoflurane. The experimental protocols were completed within 10–12 min after patch excision. To minimize channel rundown, we used the Ca2+-free and low Mg2+intracellular solution and exposed each patch to 0 ATP only at the end of experimental protocols because even a brief exposure to ATP-free solution immediately after patch excision could accelerate rundown. Therefore, the number of channels in patches could have been underestimated in our study. Channel rundown occurred more frequently at pHi 7.4. Decreasing pHi to 6.8 tended to stabilize the channels and slow rundown. Recordings from patches exhibiting a significant rundown were excluded from analysis. For measurement of ATP sensitivity, the relationship between [ATP]i and Po was fitted by Hill equation:EQUATION
where Po is channel open probability at any test [ATP]i; Pomaxis open probability at 0 [ATP]i; IC50is ATP concentration for half-maximal effect; and nH is Hill coefficient.
Statistical Analysis
Data are presented as mean ± SEM. Comparisons were made using paired or unpaired Student t  test. Multiple group means were compared by analysis of variance with a Student–Newman–Keuls test. Differences with a two-tailed P  < 0.05 were accepted as significant.
Results
The effects of isoflurane on the outward current through KATPchannels were investigated at pHi 7.4 and pHi 6.8 in the inside-out patches from guinea pig ventricular myocytes at a symmetric 140 mm K+concentration and the patch potential of +40 mV.
ATP Sensitivity of Single KATPChannels and Isoflurane Effects at pHi 7.4
Membrane patches were excised into the intracellular solution containing 0.2 mm ATP. Multiple channel openings that appeared on patch excision decreased within 30–40 s, and thereafter only the activity of spontaneously operative channels was recorded. To assess ATP dependence of isoflurane effects, we first evaluated ATP sensitivity of the channel during control conditions at pHi 7.4. The following protocol was carried out at each tested [ATP]i: control at pHi 7.4, isoflurane at pHi 7.4, washout of isoflurane at pHi 7.4, and 0 ATP at pHi 7.4. Only one concentration of ATP was tested per patch. ATPi inhibited channel activity and decreased Po in a concentration-dependent manner. Figure 1shows summary data for [ATP]i–normalized Po relationship at pHi 7.4 in the control and during application of 0.5 mm isoflurane. Each data point is a mean from four patches. During control conditions, increasing [ATP]i caused a concentration-dependent decrease in Po. Fitting mean data to the Hill equation yielded an IC50for ATP inhibition of 8 ± 1.5 μm and a Hill coefficient (nH) of 0.6 ± 0.05. When applied to the internal side of patches at pHi 7.4, isoflurane decreased Po at [ATP]i less than 50 μm but had no marked effect on Po at [ATP]i greater than 50 μm. Figure 2shows the sample traces of single KATPchannel activity recorded at pHi 7.4 in the control and during application of 0.5 mm isoflurane at 50 μm [ATP]i. In a patch containing five channels, isoflurane decreased channel activity in a reversible manner. As shown in figure 3, at 100 μm [ATP]i channel activity was much lower and little affected by isoflurane. The unitary amplitudes of 2.2 ± 0.1 pA (control) and 2.1 ± 0.1 pA (isoflurane) and the conductance were not altered by the anesthetic at pHi 7.4.
Fig. 1. Summary data for adenosine triphosphate (ATP) sensitivity of single KATPchannels at intracellular pH (pHi) 7.4. Symbols and error bars are means ± SEM. Each data point is a mean from four inside-out patches. Normalized mean open probability (Po) data obtained in control (open symbols) and in the presence of 0.5 mm isoflurane (closed symbols) are plotted against intracellular ATP ([ATP]i) concentrations. Solid line is a Hill fit (see Methods) to mean control data. The IC50for ATP inhibition and the Hill coefficient are reported in Results. At pHi 7.4, isoflurane decreased Po independently of [ATP]i. The dotted line connects mean isoflurane data points.
Fig. 1. Summary data for adenosine triphosphate (ATP) sensitivity of single KATPchannels at intracellular pH (pHi) 7.4. Symbols and error bars are means ± SEM. Each data point is a mean from four inside-out patches. Normalized mean open probability (Po) data obtained in control (open symbols) and in the presence of 0.5 mm isoflurane (closed symbols) are plotted against intracellular ATP ([ATP]i) concentrations. Solid line is a Hill fit (see Methods) to mean control data. The IC50for ATP inhibition and the Hill coefficient are reported in Results. At pHi 7.4, isoflurane decreased Po independently of [ATP]i. The dotted line connects mean isoflurane data points.
Fig. 1. Summary data for adenosine triphosphate (ATP) sensitivity of single KATPchannels at intracellular pH (pHi) 7.4. Symbols and error bars are means ± SEM. Each data point is a mean from four inside-out patches. Normalized mean open probability (Po) data obtained in control (open symbols) and in the presence of 0.5 mm isoflurane (closed symbols) are plotted against intracellular ATP ([ATP]i) concentrations. Solid line is a Hill fit (see Methods) to mean control data. The IC50for ATP inhibition and the Hill coefficient are reported in Results. At pHi 7.4, isoflurane decreased Po independently of [ATP]i. The dotted line connects mean isoflurane data points.
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Fig. 2. Effect of isoflurane on single KATPchannels at intracellular pH (pHi) 7.4. (Upper  ) Current traces recorded from an inside-out patch in the presence of 50 μm intracellular ATP ([ATP]i) at patch potential of +40 mV. The patch contained five channels, as determined at 0 ATP. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. Isoflurane (0.5 mm) applied in the intracellular solution reversibly inhibited channel activity and decreased open probability (Po) (Lower  ) All-points amplitude histograms from the recordings above demonstrate that isoflurane did not affect the amplitude of unitary current.
Fig. 2. Effect of isoflurane on single KATPchannels at intracellular pH (pHi) 7.4. (Upper 
	) Current traces recorded from an inside-out patch in the presence of 50 μm intracellular ATP ([ATP]i) at patch potential of +40 mV. The patch contained five channels, as determined at 0 ATP. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. Isoflurane (0.5 mm) applied in the intracellular solution reversibly inhibited channel activity and decreased open probability (Po) (Lower 
	) All-points amplitude histograms from the recordings above demonstrate that isoflurane did not affect the amplitude of unitary current.
Fig. 2. Effect of isoflurane on single KATPchannels at intracellular pH (pHi) 7.4. (Upper  ) Current traces recorded from an inside-out patch in the presence of 50 μm intracellular ATP ([ATP]i) at patch potential of +40 mV. The patch contained five channels, as determined at 0 ATP. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. Isoflurane (0.5 mm) applied in the intracellular solution reversibly inhibited channel activity and decreased open probability (Po) (Lower  ) All-points amplitude histograms from the recordings above demonstrate that isoflurane did not affect the amplitude of unitary current.
×
Fig. 3. Effect of isoflurane on KATPchannel activity at intracellular pH (pHi) 7.4 in the presence of 100 μm intracellular ATP ([ATP]i). (Upper  ) Shown are recordings from a patch containing two active channels as determined at 0 ATP. (C) denotes channel closed state, and upward deflection indicates the open state. At 100 μm [ATP]i, isoflurane (0.5 mm) had negligible effect on channel activity. Open probability (Po) values are shown at each experimental step. (Lower  ) All-points amplitude histograms from recordings above show no changes in unitary current amplitude with isoflurane.
Fig. 3. Effect of isoflurane on KATPchannel activity at intracellular pH (pHi) 7.4 in the presence of 100 μm intracellular ATP ([ATP]i). (Upper 
	) Shown are recordings from a patch containing two active channels as determined at 0 ATP. (C) denotes channel closed state, and upward deflection indicates the open state. At 100 μm [ATP]i, isoflurane (0.5 mm) had negligible effect on channel activity. Open probability (Po) values are shown at each experimental step. (Lower 
	) All-points amplitude histograms from recordings above show no changes in unitary current amplitude with isoflurane.
Fig. 3. Effect of isoflurane on KATPchannel activity at intracellular pH (pHi) 7.4 in the presence of 100 μm intracellular ATP ([ATP]i). (Upper  ) Shown are recordings from a patch containing two active channels as determined at 0 ATP. (C) denotes channel closed state, and upward deflection indicates the open state. At 100 μm [ATP]i, isoflurane (0.5 mm) had negligible effect on channel activity. Open probability (Po) values are shown at each experimental step. (Lower  ) All-points amplitude histograms from recordings above show no changes in unitary current amplitude with isoflurane.
×
Isoflurane Effects on ATP Sensitivity at pHi 6.8
To test whether anesthetic–KATPchannel interaction is modulated by pHi, the effects of isoflurane were examined by decreasing pHi from 7.4 to 6.8. The following protocol was carried out at each tested [ATP]i: control at pHi 7.4, control at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. This protocol also included a control baseline at pHi 7.4 because decreasing pHi itself is known to enhance opening of the cardiac KATPchannels. 12–14,16 Only one concentration of ATP was tested per patch. Figure 4shows recordings at 100 μm [ATP]i from a patch containing three active channels. Infrequent at pHi 7.4, channel opening increased markedly when decreasing pHi to 6.8. Application of 0.5 mm isoflurane at pHi 6.8 enhanced channel activity and further increased Po. Isoflurane effects were reversible during washout. Figure 5shows summary data for [ATP]i–Po relationship obtained during the above condition where each patch was sequentially exposed to the internal solution at pHi 7.4 and pHi 6.8 and to 0.5 mm isoflurane at pHi 6.8. Each data point is a mean from six patches. At pHi 7.4, fitting the [ATP]i–Po relationship to the Hill equation yielded an IC50of 8 ± 1.2 μm and nH of 0.6 ± 0.04. Decreasing pHi from 7.4 to 6.8 caused a rightward shift of the curve with IC50of 45 ± 5.6 μm and nH of 0.8 ± 0.1. The IC50value was approximately fivefold greater than that at pHi 7.4, and both values were different from each other at P  < 0.05. When applied at pHi 6.8, isoflurane further decreased ATP sensitivity, and the curve shifted further to the right, yielding an IC50of 110 ± 10.0 μm and nH of 1.05 ± 0.12. The IC50value was more than twofold greater than that at pHi 6.8 alone, and the values were significantly different at P  < 0.05. Neither decreasing pHi to 6.8 nor application of isoflurane at pHi 6.8 altered the amplitude of unitary current, which was 2.2 ± 0.1 pA at pHi 7.4, 2.3 ± 0.1 pA at pHi 6.8, and 2.3 ± 0.1 at pHi 6.8 with isoflurane. Single channel conductance remained in a range of 55–57 pS.
Fig. 4. Effect of decreasing intracellular pH (pHi) from 7.4 to 6.8 on KATPchannel activity in the absence and presence of isoflurane. (Upper  ) Sixty-second recordings of channel activity at 100 μm intracellular ATP ([ATP]i) from an inside-out patch that was exposed sequentially to intracellular solution at pHi 7.4, at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. Decreasing pHi to 6.8 increased channel activity, and during these conditions, isoflurane further enhanced channel activity. Open probability (Po) values determined at each step of the experimental protocol are shown below traces. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. (Lower  ) All-points amplitude histograms from recordings above. The amplitude of unitary outward current was not changed when decreasing pHi or during application of isoflurane at pHi 6.8.
Fig. 4. Effect of decreasing intracellular pH (pHi) from 7.4 to 6.8 on KATPchannel activity in the absence and presence of isoflurane. (Upper 
	) Sixty-second recordings of channel activity at 100 μm intracellular ATP ([ATP]i) from an inside-out patch that was exposed sequentially to intracellular solution at pHi 7.4, at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. Decreasing pHi to 6.8 increased channel activity, and during these conditions, isoflurane further enhanced channel activity. Open probability (Po) values determined at each step of the experimental protocol are shown below traces. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. (Lower 
	) All-points amplitude histograms from recordings above. The amplitude of unitary outward current was not changed when decreasing pHi or during application of isoflurane at pHi 6.8.
Fig. 4. Effect of decreasing intracellular pH (pHi) from 7.4 to 6.8 on KATPchannel activity in the absence and presence of isoflurane. (Upper  ) Sixty-second recordings of channel activity at 100 μm intracellular ATP ([ATP]i) from an inside-out patch that was exposed sequentially to intracellular solution at pHi 7.4, at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. Decreasing pHi to 6.8 increased channel activity, and during these conditions, isoflurane further enhanced channel activity. Open probability (Po) values determined at each step of the experimental protocol are shown below traces. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. (Lower  ) All-points amplitude histograms from recordings above. The amplitude of unitary outward current was not changed when decreasing pHi or during application of isoflurane at pHi 6.8.
×
Fig. 5. Summary data for intracellular ATP ([ATP]i)–normalized open probability (Po) relationship obtained at intracellular pH (pHi) 7.4, pHi 6.8, and during isoflurane application at pHi 6.8. Each data point is a mean ± SEM from six patches. Solid lines are Hill fits to the normalized Po data. Decreasing pHi from 7.4 to 6.8 shifted the curve to the right, suggesting a decrease in ATP sensitivity. At pHi 6.8, isoflurane caused further rightward shift in [ATP]i–Po relationship, suggesting further decrease in ATP sensitivity. IC50and Hill coefficient values are reported in Results.
Fig. 5. Summary data for intracellular ATP ([ATP]i)–normalized open probability (Po) relationship obtained at intracellular pH (pHi) 7.4, pHi 6.8, and during isoflurane application at pHi 6.8. Each data point is a mean ± SEM from six patches. Solid lines are Hill fits to the normalized Po data. Decreasing pHi from 7.4 to 6.8 shifted the curve to the right, suggesting a decrease in ATP sensitivity. At pHi 6.8, isoflurane caused further rightward shift in [ATP]i–Po relationship, suggesting further decrease in ATP sensitivity. IC50and Hill coefficient values are reported in Results.
Fig. 5. Summary data for intracellular ATP ([ATP]i)–normalized open probability (Po) relationship obtained at intracellular pH (pHi) 7.4, pHi 6.8, and during isoflurane application at pHi 6.8. Each data point is a mean ± SEM from six patches. Solid lines are Hill fits to the normalized Po data. Decreasing pHi from 7.4 to 6.8 shifted the curve to the right, suggesting a decrease in ATP sensitivity. At pHi 6.8, isoflurane caused further rightward shift in [ATP]i–Po relationship, suggesting further decrease in ATP sensitivity. IC50and Hill coefficient values are reported in Results.
×
Discussion
Results from this study provide direct evidence for isoflurane inhibition of ATP sensitivity of cardiac KATPchannels at reduced pHi. In the inside-out patches at pHi 6.8, isoflurane potentiates single KATPchannels by increasing Po and reduces channel sensitivity to ATP without affecting amplitude of unitary outward current and conductance.
Isoflurane alone did not activate whole cell IKATPin human atrial 6 and guinea pig ventricular myocytes;22 however, isoflurane potentiated the whole cell IKATPpreactivated by pinacidil or 2,4-dinitrophenol. 22 In guinea pig ventricular myocytes, isoflurane increased Po of KATPchannels in the cell-attached but not in the inside-out membrane patches. 8 By contrast, isoflurane decreased the activity of single KATPchannels in the inside-out patches from rabbit ventricular myocytes. 7 The pHi in all of these studies was kept at 7.4.
In the present study, in the inside-out patches and at pHi 7.4, isoflurane inhibited single KATPchannel activity and decreased Po at [ATP]i less than 50 μm in a concentration-independent manner but had no effect on channel activity at [ATP]i greater than 50 μm. This finding confirms the results of Fujimoto et al.  8, who reported lack of isoflurane effects on KATPchannel activity in the inside-out patches at 300 μm [ATP]i. We also found that isoflurane does not decrease ATP sensitivity of the channel at pHi 7.4. This observation differs from that of Han et al.  7, who reported a decrease in ATP sensitivity after exposure of inside-out patches to isoflurane at pHi 7.4. Reasons for this discrepancy are not clear, but taking aside species (rabbit vs.  guinea pig) differences, they could be related to differences in the experimental design. First, Han et al.  7 examined the effects of isoflurane at the membrane potential of −70 mV and thus investigated the inward current through KATPchannels, whereas our studies focused on the unitary outward current at the membrane potential of 0 mV (Fujimoto et al.  8) and +40 mV (present study). This raises the question whether anesthetics may differentially modulate the inward versus  outward conductance of the KATPchannel. Second, our measurements were taken during the exposure to isoflurane, whereas Han et al.  7 measured ATP sensitivity before and after application of isoflurane, as shown in their figure 1(middle and lower) and figure 3A and B. Third, from their figures 1, 2A, and 3Ait appears that ATP was absent during the patch exposure to isoflurane. Regardless of these differences, both studies have demonstrated that a volatile anesthetic, isoflurane, may directly interact with the sarcolemmal KATPchannel.
Our results suggest that pHi may be one of the factors that modulate isoflurane–channel interaction. The KATPchannels are sensitive to changes in pHi, and a decrease in pHi is a potent stimulus for their activation by the mechanism that involves a decrease in sensitivity to inhibition by ATP. The optimal effect occurs at pHi 6.8 to 6.5, and further decrease or increase in pHi leads to channel inhibition. 12,15,17–19,21 For instance, intracellular acidification to pHi less than 6.5 inhibits cardiac KATPchannels by inducing multiple subconductance levels. 15 As anticipated, in our study decreasing pHi from 7.4 to 6.8 increased channel opening. This effect was the result of increased Po and reduced ATP sensitivity, as reflected by the rightward shift in the [ATP]i –Po curve with IC50increasing from 8 μm to 45 μm. Decreasing pHi to 6.8 did not affect the amplitude of unitary outward current, as also reported by others. 12 The IC50values for ATP inhibition obtained by us at near physiologic and mild acidotic pHi are in the range of previously reported concentrations. 12,14 However, these values are not identical, and our Hill coefficients are lower. This is not surprising because the experimental conditions vary among studies, and it is well known that many factors may alter ATP sensitivity of KATPchannels. These include the variations in experimental protocols and ionic conditions, the presence and concentration of monovalent and divalent ions (Ca2+, Mg2+) and glucose, differences in the range of pHi under study, outward or inward channel conductance under study, and species differences. In addition, pHi sensitivity of KATPchannels may be modified by ATP 21 and Mg2+ions.
Our study demonstrated that mild intracellular acidosis modulates direct interaction of isoflurane with the KATPchannel. At pHi 6.8, isoflurane potentiates channel activity by decreasing ATP sensitivity and shifting IC50for ATP inhibition from 45 μm to 110 μm. The mechanism by which intracellular acidosis modulates isoflurane–KATPchannel interaction is unknown, and we can only be speculative on this point. It has been established that pH sensing is an inherent property of Kir6.2 subunits of the KATPchannel. 17 Three separate domains in the Kir6.2 protein—the N terminus, C terminus, and M2 domain—are involved in pH regulation, and the proton-sensing amino acid residues responsible for modulation of channel activity were identified in these domains. 18,19 Intracellular protons appear to increase the activity of KATPchannel by specifically binding to histidine (His-175) on the C-terminus of the Kir6.2 subunit, 18,19 and this site is independent of the ATP-binding site, lysine (K185). 17,21 Although independent, these sites appear to interact with each other, and allosteric modulation of the cloned KATPchannels by ATP and H+has been recently demonstrated. 21 Whether the allosteric modulation of channel activity by intracellular protons and ATP may play a role in isoflurane potentiation is not known. Because during ionic conditions of our study at pHi 6.8 isoflurane increased channel activity by reducing ATP sensitivity but did not affect unitary current amplitude or conductance, the channel pore is probably not targeted by the anesthetic. However, there is still a possibility of anesthetic interaction with the C terminus of the Kir6.2 subunit harboring the proton and ATP-binding sites. Furthermore, we cannot exclude a possibility of anesthetic interaction with the SUR2A subunit, modulating channel gating. 24,25 Previous findings from our laboratory and results of this study suggest that isoflurane may enhance opening of the KATPchannel previously activated or modified by the action of intracellular channel regulators, and that pHi may be one of them.
The functional significance of the mitochondrial versus  sarcolemmal KATPchannels for cardioprotection remains controversial. Recent evidence suggests a role for mitochondrial KATPchannels in the initiation of cardioprotection, 26 but sarcolemmal KATPchannels have also been indicated in the protection afforded by ischemic preconditioning. 27 Activation of the KATPchannels is thought to be crucial for anesthetic preconditioning. However, the precise mechanism by which the enhancement of KATPchannel activity by volatile anesthetics protects the myocardium is not yet established. Cardiac KATPchannels are closed at physiologic [ATP]i. During pathophysiologic conditions, such as ischemia, KATPchannels activate during the first few minutes of the ischemic insult, 28 long before any significant decrease in [ATP]i. 29 This suggested that other intracellular factors must be involved, and modulation of ATP sensitivity by factors targeting specifically the SUR subunit or the Kir6.x subunit has been demonstrated for ADP, 24,25 PIP2, 30,31 and pHi. 17,21 A transient decrease in pHi that occurs during ischemia 32–34 and accompanies other metabolic stresses may promote opening of sarcolemmal KATPchannels by decreasing sensitivity to ATP, thus allowing isoflurane interaction with the channel, leading to further enhancement of channel activity. Recent studies implicated an important role of the adenylate kinase and creatine kinase-mediated phosphotransfer in communicating mitochondria-generated signals to the sarcolemmal KATPchannels. 35,36 Whether volatile anesthetics alter the signal transfer to the cell subsarcolemmal compartment and KATPchannels is an open question.
Our results support a possible role of volatile anesthetics in ischemia because of early ischemic acidosis. 32 However, as demonstrated in the animal models and in humans, volatile anesthetics precondition the myocardium independently of ischemia. 3,6,37–39 In clinical settings, the pHi dependence of KATPchannel potentiation by isoflurane would likely play a protective role perioperatively when various metabolic stresses may produce a transient decrease in pHi.
In conclusion, this study provides evidence for pHi-dependent modulation of direct isoflurane interaction with the cardiac sarcolemmal KATPchannel in guinea pig ventricular myocytes. Although at near physiologic pHi isoflurane has no effect nor inhibits KATPchannel, at reduced pHi of 6.8, isoflurane increases channel Po and decreases sensitivity to inhibition by ATP as reflected by the rightward shift of the [ATP]i–Po relationship.
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Fig. 1. Summary data for adenosine triphosphate (ATP) sensitivity of single KATPchannels at intracellular pH (pHi) 7.4. Symbols and error bars are means ± SEM. Each data point is a mean from four inside-out patches. Normalized mean open probability (Po) data obtained in control (open symbols) and in the presence of 0.5 mm isoflurane (closed symbols) are plotted against intracellular ATP ([ATP]i) concentrations. Solid line is a Hill fit (see Methods) to mean control data. The IC50for ATP inhibition and the Hill coefficient are reported in Results. At pHi 7.4, isoflurane decreased Po independently of [ATP]i. The dotted line connects mean isoflurane data points.
Fig. 1. Summary data for adenosine triphosphate (ATP) sensitivity of single KATPchannels at intracellular pH (pHi) 7.4. Symbols and error bars are means ± SEM. Each data point is a mean from four inside-out patches. Normalized mean open probability (Po) data obtained in control (open symbols) and in the presence of 0.5 mm isoflurane (closed symbols) are plotted against intracellular ATP ([ATP]i) concentrations. Solid line is a Hill fit (see Methods) to mean control data. The IC50for ATP inhibition and the Hill coefficient are reported in Results. At pHi 7.4, isoflurane decreased Po independently of [ATP]i. The dotted line connects mean isoflurane data points.
Fig. 1. Summary data for adenosine triphosphate (ATP) sensitivity of single KATPchannels at intracellular pH (pHi) 7.4. Symbols and error bars are means ± SEM. Each data point is a mean from four inside-out patches. Normalized mean open probability (Po) data obtained in control (open symbols) and in the presence of 0.5 mm isoflurane (closed symbols) are plotted against intracellular ATP ([ATP]i) concentrations. Solid line is a Hill fit (see Methods) to mean control data. The IC50for ATP inhibition and the Hill coefficient are reported in Results. At pHi 7.4, isoflurane decreased Po independently of [ATP]i. The dotted line connects mean isoflurane data points.
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Fig. 2. Effect of isoflurane on single KATPchannels at intracellular pH (pHi) 7.4. (Upper  ) Current traces recorded from an inside-out patch in the presence of 50 μm intracellular ATP ([ATP]i) at patch potential of +40 mV. The patch contained five channels, as determined at 0 ATP. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. Isoflurane (0.5 mm) applied in the intracellular solution reversibly inhibited channel activity and decreased open probability (Po) (Lower  ) All-points amplitude histograms from the recordings above demonstrate that isoflurane did not affect the amplitude of unitary current.
Fig. 2. Effect of isoflurane on single KATPchannels at intracellular pH (pHi) 7.4. (Upper 
	) Current traces recorded from an inside-out patch in the presence of 50 μm intracellular ATP ([ATP]i) at patch potential of +40 mV. The patch contained five channels, as determined at 0 ATP. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. Isoflurane (0.5 mm) applied in the intracellular solution reversibly inhibited channel activity and decreased open probability (Po) (Lower 
	) All-points amplitude histograms from the recordings above demonstrate that isoflurane did not affect the amplitude of unitary current.
Fig. 2. Effect of isoflurane on single KATPchannels at intracellular pH (pHi) 7.4. (Upper  ) Current traces recorded from an inside-out patch in the presence of 50 μm intracellular ATP ([ATP]i) at patch potential of +40 mV. The patch contained five channels, as determined at 0 ATP. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. Isoflurane (0.5 mm) applied in the intracellular solution reversibly inhibited channel activity and decreased open probability (Po) (Lower  ) All-points amplitude histograms from the recordings above demonstrate that isoflurane did not affect the amplitude of unitary current.
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Fig. 3. Effect of isoflurane on KATPchannel activity at intracellular pH (pHi) 7.4 in the presence of 100 μm intracellular ATP ([ATP]i). (Upper  ) Shown are recordings from a patch containing two active channels as determined at 0 ATP. (C) denotes channel closed state, and upward deflection indicates the open state. At 100 μm [ATP]i, isoflurane (0.5 mm) had negligible effect on channel activity. Open probability (Po) values are shown at each experimental step. (Lower  ) All-points amplitude histograms from recordings above show no changes in unitary current amplitude with isoflurane.
Fig. 3. Effect of isoflurane on KATPchannel activity at intracellular pH (pHi) 7.4 in the presence of 100 μm intracellular ATP ([ATP]i). (Upper 
	) Shown are recordings from a patch containing two active channels as determined at 0 ATP. (C) denotes channel closed state, and upward deflection indicates the open state. At 100 μm [ATP]i, isoflurane (0.5 mm) had negligible effect on channel activity. Open probability (Po) values are shown at each experimental step. (Lower 
	) All-points amplitude histograms from recordings above show no changes in unitary current amplitude with isoflurane.
Fig. 3. Effect of isoflurane on KATPchannel activity at intracellular pH (pHi) 7.4 in the presence of 100 μm intracellular ATP ([ATP]i). (Upper  ) Shown are recordings from a patch containing two active channels as determined at 0 ATP. (C) denotes channel closed state, and upward deflection indicates the open state. At 100 μm [ATP]i, isoflurane (0.5 mm) had negligible effect on channel activity. Open probability (Po) values are shown at each experimental step. (Lower  ) All-points amplitude histograms from recordings above show no changes in unitary current amplitude with isoflurane.
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Fig. 4. Effect of decreasing intracellular pH (pHi) from 7.4 to 6.8 on KATPchannel activity in the absence and presence of isoflurane. (Upper  ) Sixty-second recordings of channel activity at 100 μm intracellular ATP ([ATP]i) from an inside-out patch that was exposed sequentially to intracellular solution at pHi 7.4, at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. Decreasing pHi to 6.8 increased channel activity, and during these conditions, isoflurane further enhanced channel activity. Open probability (Po) values determined at each step of the experimental protocol are shown below traces. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. (Lower  ) All-points amplitude histograms from recordings above. The amplitude of unitary outward current was not changed when decreasing pHi or during application of isoflurane at pHi 6.8.
Fig. 4. Effect of decreasing intracellular pH (pHi) from 7.4 to 6.8 on KATPchannel activity in the absence and presence of isoflurane. (Upper 
	) Sixty-second recordings of channel activity at 100 μm intracellular ATP ([ATP]i) from an inside-out patch that was exposed sequentially to intracellular solution at pHi 7.4, at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. Decreasing pHi to 6.8 increased channel activity, and during these conditions, isoflurane further enhanced channel activity. Open probability (Po) values determined at each step of the experimental protocol are shown below traces. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. (Lower 
	) All-points amplitude histograms from recordings above. The amplitude of unitary outward current was not changed when decreasing pHi or during application of isoflurane at pHi 6.8.
Fig. 4. Effect of decreasing intracellular pH (pHi) from 7.4 to 6.8 on KATPchannel activity in the absence and presence of isoflurane. (Upper  ) Sixty-second recordings of channel activity at 100 μm intracellular ATP ([ATP]i) from an inside-out patch that was exposed sequentially to intracellular solution at pHi 7.4, at pHi 6.8, isoflurane at pHi 6.8, washout of isoflurane at pHi 6.8, and 0 ATP at pHi 6.8. Decreasing pHi to 6.8 increased channel activity, and during these conditions, isoflurane further enhanced channel activity. Open probability (Po) values determined at each step of the experimental protocol are shown below traces. Dashed lines denote the closed state (C). Upward deflection indicates channel opening. (Lower  ) All-points amplitude histograms from recordings above. The amplitude of unitary outward current was not changed when decreasing pHi or during application of isoflurane at pHi 6.8.
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Fig. 5. Summary data for intracellular ATP ([ATP]i)–normalized open probability (Po) relationship obtained at intracellular pH (pHi) 7.4, pHi 6.8, and during isoflurane application at pHi 6.8. Each data point is a mean ± SEM from six patches. Solid lines are Hill fits to the normalized Po data. Decreasing pHi from 7.4 to 6.8 shifted the curve to the right, suggesting a decrease in ATP sensitivity. At pHi 6.8, isoflurane caused further rightward shift in [ATP]i–Po relationship, suggesting further decrease in ATP sensitivity. IC50and Hill coefficient values are reported in Results.
Fig. 5. Summary data for intracellular ATP ([ATP]i)–normalized open probability (Po) relationship obtained at intracellular pH (pHi) 7.4, pHi 6.8, and during isoflurane application at pHi 6.8. Each data point is a mean ± SEM from six patches. Solid lines are Hill fits to the normalized Po data. Decreasing pHi from 7.4 to 6.8 shifted the curve to the right, suggesting a decrease in ATP sensitivity. At pHi 6.8, isoflurane caused further rightward shift in [ATP]i–Po relationship, suggesting further decrease in ATP sensitivity. IC50and Hill coefficient values are reported in Results.
Fig. 5. Summary data for intracellular ATP ([ATP]i)–normalized open probability (Po) relationship obtained at intracellular pH (pHi) 7.4, pHi 6.8, and during isoflurane application at pHi 6.8. Each data point is a mean ± SEM from six patches. Solid lines are Hill fits to the normalized Po data. Decreasing pHi from 7.4 to 6.8 shifted the curve to the right, suggesting a decrease in ATP sensitivity. At pHi 6.8, isoflurane caused further rightward shift in [ATP]i–Po relationship, suggesting further decrease in ATP sensitivity. IC50and Hill coefficient values are reported in Results.
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