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Meeting Abstracts  |   April 2006
Intravenous Anesthetics Inhibit Capacitative Calcium Entry in Pulmonary Venous Smooth Muscle Cells
Author Affiliations & Notes
  • Sachiko Shimizu, M.D.
    *
  • Xueqin Ding, M.D., Ph.D.
    *
  • Paul A. Murray, Ph.D.
  • * Research Fellow, † Carl E. Wasmuth Endowed Chair and Director.
Article Information
Meeting Abstracts   |   April 2006
Intravenous Anesthetics Inhibit Capacitative Calcium Entry in Pulmonary Venous Smooth Muscle Cells
Anesthesiology 4 2006, Vol.104, 791-797. doi:
Anesthesiology 4 2006, Vol.104, 791-797. doi:
CAPACITATIVE calcium entry (CCE) is activated by depletion of intracellular Ca2+stores.1,2 It is a critical mechanism for refilling intracellular Ca2+stores and maintaining a sustained increase in intracellular Ca2+concentration ([Ca2+]i).3,4 CCE may also be of importance in the regulation of a number of diverse cellular functions, such as apoptosis, secretion, and gene transcription.5 Furthermore, it has been suggested that CCE plays an important role in agonist-mediated pulmonary artery contraction.4 We have previously demonstrated that CCE exits and is involved in [Ca2+]ioscillations as well as the contractile response induced by α1-adrenoreceptor activation in pulmonary artery smooth muscle cells (PASMCs).6 
Pulmonary veins are a primary site for entry of vagal nerves into the left atrium7 and are likely involved in atrial fibrillation.8 Pulmonary venous constriction results in pulmonary edema formation in congestive heart failure,9 as well as in high-altitude pulmonary edema.10 Pulmonary veins are known to constrict in response to a number of stimuli.11–13 An increase in [Ca2+]iis a major trigger for pulmonary venous constriction.12,13 However, the role of CCE in the regulation of [Ca2+]iin pulmonary venous smooth muscle cells (PVSMCs) is unknown. Moreover, the effects of intravenous anesthetics on CCE in PVSMCs have not been elucidated.
Our first goal was to determine whether CCE exists in PVSMCs. We have previously demonstrated that tyrosine kinase (TK) positively regulates CCE,6 whereas protein kinase C (PKC) negatively14 regulates CCE in PASMCs. Our second goal was to investigate the role of the TK, PKC, and ρ-kinase (ROK) signaling pathways in regulating CCE in PVSMCs. We have previously reported that propofol attenuates CCE via  the PKC signaling pathway in PASMCs.14 Therefore, our third goal was to investigate the effects of intravenous anesthetics (ketamine, thiopental, midazolam, and propofol) on CCE in PVSMCs and to identify the signaling pathways involved in anesthesia-induced changes in CCE in PVSMCs.
Materials and Methods
Animals
Pulmonary veins were isolated from adult mongrel dogs. The technique of euthanasia was approved by the Cleveland Clinic Institutional Animal Care and Use Committee (Cleveland, Ohio). All steps were performed aseptically during general anesthesia with intravenous pentobarbital sodium (30 mg/kg) and intravenous fentanyl citrate (20 μg/kg). The dogs were intubated and ventilated. After administration of heparin (6,000 U), the dogs were exsanguinated by controlled hemorrhage via  a femoral artery catheter and killed with electrically induced ventricular fibrillation. A left lateral thoracotomy was performed, and the heart and lungs were removed en bloc  . The pulmonary veins ware isolated and dissected in a laminar flow hood using sterile procedures.
Cell Culture of PVSMCs
Primary cultures of PVSMCs were obtained from segmental and subsegmental branches of pulmonary vein (the third and fourth generations having diameters < 4 mm). The intralobar veins were carefully dissected and prepared for tissue culture. Explant cultures were prepared according to the method of Campbell and Campbell,15 with minor modifications. Briefly, the endothelium was removed by gently rubbing with a sterile cotton swab. The tunica adventitia was carefully removed, together with the most superficial part of the tunica media. The remaining part of the media was cut into 2-mm2pieces that were explanted in 25-cm2culture flasks. The explants were nourished by Dulbecco's modified Eagle medium/F-12 containing 10% fetal bovine serum and 1% antibiotic mixture solution (10,000 U/ml penicillin and 10,000 μg/ml streptomycin) and kept in a humidified atmosphere of 5% CO2–95% air at 37°C. PVSMCs began to proliferate from explants after 7 days in culture. Cells were allowed to grow for an additional 10–14 days until subconfluence was achieved. Cells were then subcultured to 35-mm glass dishes specially designed for fluorescence microscopy (Bioptechs ΔT system; Butler, PA). Cells from the first passage were used for experiments. The cells exhibited morphologic characteristics of vascular smooth muscle and expressed α-actin as assessed by Western blot analysis.
Fura-2 Loading Procedure
Twenty-four hours before experimentation, the culture medium containing 10% fetal bovine serum was replaced with serum-free medium to arrest cell growth, to allow for establishment of steady state cellular events independent of cell division, and to prevent a false estimate of [Ca2+]iresulting from binding of available dye to serum protein in the medium. PVSMCs were loaded with the acetoxymethyl ester form of fura-2 (fura-2 AM: 2 μm) at ambient temperature. After the 30-min loading period, the cells were washed twice in Krebs-Ringer's buffer, which contained 125 mm NaCl, 5 mm KCl, 1.2 mm MgSO4, 11 mm glucose, 2.5 mm CaCl2, and 25 mm HEPES at pH 7.40 adjusted with NaOH at ambient temperature for an additional 20 min before initiating the study. This provided enough time to wash away any extracellular fura-2 AM and for intracellular esterases to cleave fura-2 AM into the active fura-2.
Measurement of Intracellular Ca2+Concentration
Intracellular Ca2+concentration was measured as previously described.6 Culture dishes containing fura-2–loaded PVSMCs were placed in a temperature-regulated (37°C) chamber (Bioptechs, Inc., Butler, PA) mounted on the stage of an Olympus IX-70 inverted fluorescence microscope (Olympus America Inc., Lake Success, NY). Fluorescence measurements were obtained from individual PVSMCs in a culture monolayer using a dual-wavelength spectrofluorometer (Deltascan RFK6002; Photon Technology International, Lawrenceville, NJ) at excitation wavelengths of 340 and 380 nm and an emission wavelength of 510 nm. The volume of the chamber was 1.5 ml. The temperature of all solutions was maintained at 37°C in a water bath. Solution changes were accomplished rapidly by aspirating the buffer in the dish and superfusing it with a pipet. Just before data acquisition, background fluorescence (i.e.  , fluorescence between cells) was measured and subtracted automatically from the subsequent experimental measurements. Fura-2 fluorescence signals (340, 380, and 340/380 ratio) originating from PVSMCs were continuously monitored at a sampling frequency of 25 Hz and were collected using a software package from Photon Technology International.
Experimental Protocols
In the absence of extracellular Ca2+, thapsigargin was used to deplete intracellular Ca2+stores. Thapsigargin is an irreversible sarcoplasmic reticulum Ca2+-adenosine triphosphatase inhibitor and can induce CCE.16 After depletion of sarcoplasmic reticulum Ca2+stores, CCE was induced when extracellular Ca2+([Ca2+]o, 2.2 mm) was restored. The effects of L-type voltage dependent Ca2+channel inhibition (verapamil, 10 μm), nonselective Ca2+channel inhibition (SKF 96365, 50 μm), TK inhibition (tyrphostin 23, 100 μm), PKC activation (phorbol 12-myristate 13-acetate, 1 μm), PKC inhibition (bisindolylmaleimide I, 1 μm), and ROK inhibition (Y27632, 10 μm) on CCE were investigated. The concentration of the inhibitors was chosen based on previous experience in PASMCs6,14 and PVSMCs.12,13 The effects of intravenous anesthetics (thiopental, 10–100 μm; midazolam, 10–100 μm; ketamine, 10–100 μm; and propofol, 10–100 μm), alone or in combination with a signaling pathway inhibitor, on CCE were assessed.
Drug Preparation
Verapamil, SKF 96365, tyrphostin 23, phorbol 12-myristate 13-acetate, bisindolylmaleimide (Sigma, St. Louis, MO), and propofol (Aldrich Chemical Co., Milwaukee, WI) were dissolved in dimethyl sulfoxide. The final chamber concentration of dimethyl sulfoxide was less than 0.1% (vol/vol). This diluent had no effect on CCE at the concentration used in these studies. Y27632 (Calbiochem, La Jolla, CA), ketamine (Fort Dodge Animal Health, Fort Dodge, IA), midazolam (American Pharmaceutical Partners Inc., Schaumburg, IL), and thiopental (Sigma) were dissolved in distilled water.
Data Analysis
Data analysis was performed as previously described.14 Peak and sustained increases in [Ca2+]iwere measured in PVSMCs when the superfusion solution was switched from a Ca2+-free solution to a solution containing 2.2 mm Ca2+. Peak and sustained fluorescence ratio values were averaged before and after each intervention and are expressed as percent of control. The control response to which all interventions were compared was the first CCE response after thapsigargin pretreatment. This value was set at 100%. Therefore, each cell served as it own control. The peak response was calculated as the fluorescence change from baseline to peak fluorescence. The sustained response represents the fluorescence values measured when the 340/380 ratio was stable after reintroduction of Ca2+to the buffer. Results are presented as mean ± SEM. Statistical analysis was performed with analysis of variance and the Student t  test. Differences were considered statistically significant at P  < 0.05.
Results
CCE in PVSMCs
To identify the presence of CCE in PVSMCs, thapsigargin was used to deplete sarcoplasmic reticulum Ca2+stores in the absence of extracellular Ca2+. Thapsigargin transiently increased [Ca2+]iby 160 ± 6%, which gradually returned to baseline. Restoring extracellular Ca2+([Ca2+]o, 2.2 mm) then caused a rapid peak increase in [Ca2+]i(155 ± 7% of baseline; P  < 0.05), followed by a sustained increase in [Ca2+]i(129 ± 5% of baseline; P  < 0.05), i.e.  , CCE was stimulated in pulmonary venous smooth muscle cells (fig. 1A). The sustained increase in [Ca2+]ireturned to baseline when [Ca2+]owas removed. To identify the reproducibility of inducing CCE in the same PVSMC, [Ca2+]owas sequentially restored and removed three consecutive times. There were no significant differences in the peak or sustained increases in [Ca2+]ibetween the first and the second CCE, but the third CCE was slightly smaller in magnitude in the peak and sustained increases in [Ca2+]icompared with the first CCE (fig. 1B).
Fig. 1. (  A  ) Representative trace depicting capacitative Ca2+entry after depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin in pulmonary venous smooth muscle cells. Extracellular Ca2+was sequentially added and removed three times.  (B  ) Summarized data showing the reproducibility of capacitative Ca2+entry. The third capacitative Ca2+entry response was decreased (*  P  < 0.05) slightly compared with the first capacitative Ca2+entry response. n = 13  .
Fig. 1. (  A  ) Representative trace depicting capacitative Ca2+entry after depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin in pulmonary venous smooth muscle cells. Extracellular Ca2+was sequentially added and removed three times. 
	(B  ) Summarized data showing the reproducibility of capacitative Ca2+entry. The third capacitative Ca2+entry response was decreased (*  P  < 0.05) slightly compared with the first capacitative Ca2+entry response. n = 13 
	.
Fig. 1. (  A  ) Representative trace depicting capacitative Ca2+entry after depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin in pulmonary venous smooth muscle cells. Extracellular Ca2+was sequentially added and removed three times.  (B  ) Summarized data showing the reproducibility of capacitative Ca2+entry. The third capacitative Ca2+entry response was decreased (*  P  < 0.05) slightly compared with the first capacitative Ca2+entry response. n = 13  .
×
Effect of Receptor-operated Ca2+Channel Inhibition on CCE
SKF 96365 is a nonselective Ca2+channel inhibitor that has been used by many investigators to inhibit CCE.17 SKF 96365 (50 μm) was applied 5 min before [Ca2+]owas restored the second time (fig. 2A). SKF 96365 attenuated both the peak and sustained increases in [Ca2+]idue to CCE (fig. 2B).
Fig. 2. (  A  ) Representative trace depicting the effect of the nonselective Ca2+channel blocker SKF 96365 (50 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of SKF 96365, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of the voltage-dependent Ca2+channel blocker verapamil (10 μm, n = 7) and SKF 96365 (50 μm, n = 9) on capacitative Ca2+entry, respectively. *  P  < 0.05 compared with control  .
Fig. 2. (  A  ) Representative trace depicting the effect of the nonselective Ca2+channel blocker SKF 96365 (50 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of SKF 96365, which was added to the buffer 5 min before restoring extracellular Ca2+concentration. 
	(B  ) Summarized data showing the effects of the voltage-dependent Ca2+channel blocker verapamil (10 μm, n = 7) and SKF 96365 (50 μm, n = 9) on capacitative Ca2+entry, respectively. *  P  < 0.05 compared with control 
	.
Fig. 2. (  A  ) Representative trace depicting the effect of the nonselective Ca2+channel blocker SKF 96365 (50 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of SKF 96365, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of the voltage-dependent Ca2+channel blocker verapamil (10 μm, n = 7) and SKF 96365 (50 μm, n = 9) on capacitative Ca2+entry, respectively. *  P  < 0.05 compared with control  .
×
Effect of Voltage-operated Ca2+Channel Inhibition on CCE
Verapamil was used to inhibit voltage-dependent Ca2+channels. Verapamil (10 μm) was applied 5 min before [Ca2+]owas restored the second time. Verapamil had no effect on the peak or sustained increases in [Ca2+]idue to CCE (fig. 2B).
Effect of TK Inhibition on CCE
We previously demonstrated that TK plays a role in regulating CCE in PASMCs.6,14 Tyrphostin 23 was used to inhibit TK. Tyrphostin 23 (100 μm) was applied 5 min before [Ca2+]owas restored the second time (fig. 3A). Tyrphostin 23 attenuated both the peak and sustained increases in [Ca2+]idue to CCE (fig. 3B).
Fig. 3. (  A  ) Representative trace depicting the effect of the tyrosine kinase inhibitor tyrphostin 23 (Tyr 23: 100 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of tyrphostin 23, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of tyrphostin 23 (Tyr 23, 100 μm, n = 9), the protein kinase C activator phorbol 12-myristate 13-acetate (PMA, 1 μm, n = 16), the protein kinase C inhibitor bisindolylmaleimide I (BIS 1, 1 μm, n = 6), and the ρ-kinase inhibitor Y27632 (10 μm, n = 6) on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
Fig. 3. (  A  ) Representative trace depicting the effect of the tyrosine kinase inhibitor tyrphostin 23 (Tyr 23: 100 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of tyrphostin 23, which was added to the buffer 5 min before restoring extracellular Ca2+concentration. 
	(B  ) Summarized data showing the effects of tyrphostin 23 (Tyr 23, 100 μm, n = 9), the protein kinase C activator phorbol 12-myristate 13-acetate (PMA, 1 μm, n = 16), the protein kinase C inhibitor bisindolylmaleimide I (BIS 1, 1 μm, n = 6), and the ρ-kinase inhibitor Y27632 (10 μm, n = 6) on capacitative Ca2+entry. *  P  < 0.05 compared with control 
	.
Fig. 3. (  A  ) Representative trace depicting the effect of the tyrosine kinase inhibitor tyrphostin 23 (Tyr 23: 100 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of tyrphostin 23, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of tyrphostin 23 (Tyr 23, 100 μm, n = 9), the protein kinase C activator phorbol 12-myristate 13-acetate (PMA, 1 μm, n = 16), the protein kinase C inhibitor bisindolylmaleimide I (BIS 1, 1 μm, n = 6), and the ρ-kinase inhibitor Y27632 (10 μm, n = 6) on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
×
Effects of PKC Activation and Inhibition on CCE
We previously demonstrated that PKC plays a role in regulating CCE in PASMCs.14 Phorbol 12-myristate 13-acetate (1 μm) and bisindolylmaleimide I (1 μm) were used to activate and inhibit PKC, respectively. They were applied 5 min before [Ca2+]owas restored the second time. Neither phorbol 12-myristate 13-acetate nor bisindolylmaleimide I had an effect on the peak or sustained increases in [Ca2+]idue to CCE (fig. 3B).
Effect of ROK Inhibition on CCE
Y27632 was used to inhibit ROK. Y27632 (10 μm) was applied 5 min before [Ca2+]owas restored the second time. Y27632 had no effect on the peak or sustained increases in [Ca2+]idue to CCE (fig. 3B).
Effects of Intravenous Anesthetics on CCE
The intravenous anesthetics were applied 15 min before [Ca2+]owas restored the second time. Ketamine (10–100 μm) caused dose-dependent decreases in both the peak and sustained increases in [Ca2+]idue to CCE (fig. 4A). Thiopental (30–100 μm) caused dose-dependent decreases in both the peak and sustained increases in [Ca2+]idue to CCE (fig. 4B), although the lowest concentration of thiopental had no effect (fig. 4B). Midazolam (30–100 μm) caused dose-dependent decreases in both the peak and sustained increases in [Ca2+]idue to CCE (fig. 5A), whereas the lowest concentration of midazolam (10 μm) had no effect (fig. 5A). Propofol (100 μm) attenuated both the peak and sustained increases in [Ca2+]idue to CCE (fig. 5B), but lower concentrations of propofol (30 μm, 10 μm) had no effect (fig. 5B).
Fig. 4. (  A  ) Summarized data showing the dose-dependent inhibitory effects of ketamine (10 μm, n = 9; 30 μm, n = 9; 100 μm, n = 8) on capacitative Ca2+entry.  (B  ) Summarized data showing the dose-dependent inhibitory effects of thiopental (30 μm, n = 9; 100 μm, n = 10) on capacitative Ca2+entry. However, 10 μm thiopental had no effect on capacitative Ca2+entry (n = 9). *  P  < 0.05 compared with control  .
Fig. 4. (  A  ) Summarized data showing the dose-dependent inhibitory effects of ketamine (10 μm, n = 9; 30 μm, n = 9; 100 μm, n = 8) on capacitative Ca2+entry. 
	(B  ) Summarized data showing the dose-dependent inhibitory effects of thiopental (30 μm, n = 9; 100 μm, n = 10) on capacitative Ca2+entry. However, 10 μm thiopental had no effect on capacitative Ca2+entry (n = 9). *  P  < 0.05 compared with control 
	.
Fig. 4. (  A  ) Summarized data showing the dose-dependent inhibitory effects of ketamine (10 μm, n = 9; 30 μm, n = 9; 100 μm, n = 8) on capacitative Ca2+entry.  (B  ) Summarized data showing the dose-dependent inhibitory effects of thiopental (30 μm, n = 9; 100 μm, n = 10) on capacitative Ca2+entry. However, 10 μm thiopental had no effect on capacitative Ca2+entry (n = 9). *  P  < 0.05 compared with control  .
×
Fig. 5. (  A  ) Summarized data showing the dose-dependent inhibitory effects of midazolam (30 μm, n = 6; 100 μm, n = 9) on capacitative Ca2+entry. However, 10 μm midazolam had no effect on capacitative Ca2+entry (n = 6).  (B  ) Summarized data showing the inhibitory effects of 100 μm propofol on capacitative Ca2+entry (n = 8). However, lower concentrations of propofol (10 μm, n = 6; 30 μm, n = 7) had no effect on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
Fig. 5. (  A  ) Summarized data showing the dose-dependent inhibitory effects of midazolam (30 μm, n = 6; 100 μm, n = 9) on capacitative Ca2+entry. However, 10 μm midazolam had no effect on capacitative Ca2+entry (n = 6). 
	(B  ) Summarized data showing the inhibitory effects of 100 μm propofol on capacitative Ca2+entry (n = 8). However, lower concentrations of propofol (10 μm, n = 6; 30 μm, n = 7) had no effect on capacitative Ca2+entry. *  P  < 0.05 compared with control 
	.
Fig. 5. (  A  ) Summarized data showing the dose-dependent inhibitory effects of midazolam (30 μm, n = 6; 100 μm, n = 9) on capacitative Ca2+entry. However, 10 μm midazolam had no effect on capacitative Ca2+entry (n = 6).  (B  ) Summarized data showing the inhibitory effects of 100 μm propofol on capacitative Ca2+entry (n = 8). However, lower concentrations of propofol (10 μm, n = 6; 30 μm, n = 7) had no effect on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
×
Effect of TK Inhibition on Anesthesia-induced Attenuation of CCE
To determine whether the TK signaling pathway is involved in the anesthesia-induced attenuation of CCE, we investigated the effects of the anesthetics on CCE in the presence of TK inhibition. The intravenous anesthetics were applied before [Ca2+]owas restored the second time. In the presence of tyrphostin 23, propofol further attenuated both the peak and sustained increases in [Ca2+]idue to CCE compared with TK inhibition alone (fig. 6). However, ketamine, thiopental, and midazolam had no effect on the peak or sustained increases in [Ca2+]idue to CCE in the presence of TK inhibition compared with TK inhibition alone (fig. 6).
Fig. 6. Summarized data showing the effects of the intravenous anesthetics on capacitative Ca2+entry in the presence of tyrphostin 23 (Tyr 23). In the presence of Tyr 23, ketamine (100 μm, n = 6), thiopental (100 μm, n = 6), and midazolam (100 μm, n = 6) no longer had an inhibitory effect on capacitative Ca2+entry. However, propofol (100 μm, n = 6) continued to decrease capacitative Ca2+entry after pretreatment with Tyr 23. *  P  < 0.05 compared with Tyr23 alone  .
Fig. 6. Summarized data showing the effects of the intravenous anesthetics on capacitative Ca2+entry in the presence of tyrphostin 23 (Tyr 23). In the presence of Tyr 23, ketamine (100 μm, n = 6), thiopental (100 μm, n = 6), and midazolam (100 μm, n = 6) no longer had an inhibitory effect on capacitative Ca2+entry. However, propofol (100 μm, n = 6) continued to decrease capacitative Ca2+entry after pretreatment with Tyr 23. *  P  < 0.05 compared with Tyr23 alone 
	.
Fig. 6. Summarized data showing the effects of the intravenous anesthetics on capacitative Ca2+entry in the presence of tyrphostin 23 (Tyr 23). In the presence of Tyr 23, ketamine (100 μm, n = 6), thiopental (100 μm, n = 6), and midazolam (100 μm, n = 6) no longer had an inhibitory effect on capacitative Ca2+entry. However, propofol (100 μm, n = 6) continued to decrease capacitative Ca2+entry after pretreatment with Tyr 23. *  P  < 0.05 compared with Tyr23 alone  .
×
Discussion
Our results demonstrate that CCE exists in canine PVSMCs. The TK signaling pathway, but not the PKC and ROK pathways, is involved in CCE in canine PVSMCs. Clinically relevant concentrations of ketamine and thiopental attenuate CCE, whereas only supraclinical concentrations of midazolam and propofol have this effect. The TK signaling pathway is involved in the ketamine-, thiopental-, and midazolam-induced attenuation of CCE, whereas it is not involved in the propofol-induced attenuation of CCE.
CCE in PVSMCs
Capacitative Ca2+entry has been demonstrated in a variety of cell types, including vascular smooth muscle cells.18,19 In the current study, we used thapsigargin to deplete the sarcoplasmic reticulum pool of Ca2+in the absence of extracellular Ca2+and thereby activated CCE.20 Restoring [Ca2+]ocaused a rapid peak increase followed by a sustained increase in [Ca2+]i. This is the first demonstration that CCE exists in PVSMCs. When [Ca2+]iwas restored and removed three times, there were no differences between the first and second CCE responses, but the third CCE was slightly reduced. Therefore, we assessed the effects of interventions by comparing the second CCE response to the first.
Effects of SKF 96365 and Verapamil on CCE
SKF 96365 has been used to block CCE after depletion of sarcoplasmic reticulum Ca2+stores in a variety of cell types.17 In our study, SKF 96365 markedly attenuated both the peak and sustained increases in [Ca2+]idue to CCE, whereas verapamil had no effect. These results are consistent with the concept that CCE is insensitive to voltage-gated Ca2+channel inhibitors.
Effect of PKC and ROK Inhibition on CCE
The basis of CCE is that release of Ca2+from intracellular stores increases Ca2+influx. The mechanism linking the decrease in intracellular Ca2+stores to the opening of plasma membrane Ca2+channels remains controversial. One theory postulates the release of a diffusible messenger by the pools, whereas other hypotheses involve a physical interaction between the empty stores and plasma membrane proteins, secretory vesicles, or even cytoskeletal elements.21 It has been proposed that the sarcoplasmic reticulum might possess protein kinases or phosphatases capable of altering the phosphorylation state of ion channels.22 Previous studies reported that PKC activation could inhibit23 or facilitate24 CCE. We have demonstrated that PKC negatively regulates CCE in PASMCs.14 In the current study, neither the PKC activator, phorbol 12-myristate 13-acetate, nor the PKC inhibitor, bisindolylmaleimide I, had an effect on CCE in PVSMCs. We also assessed the role of another kinase, ROK, in CCE. We have recently reported that ROK is involved in agonist-induced pulmonary venous contraction12,13 However, Y27632, a ROK inhibitor, had no effect on CCE in PVSMCs. This suggests that the ROK signaling pathway is not involved in CCE in PVSMCs.
Role of TK in CCE in PVSMCs
It has been reported that depletion of intracellular Ca2+stores triggers tyrosine phosphorylation,25 and inhibition of TK attenuates CCE in a number of cell types,26,27 including smooth muscle.28 We have demonstrated that inhibition of TK attenuates CCE in PASMCs.6,14 In the current study, the TK inhibitor, tyrphostin 23, attenuated CCE in PVSMCs. This suggests that the TK signaling pathway is involved in CCE in PVSMCs.
Effects of Intravenous Anesthetics on CCE in PVSMCs
It is well known that [Ca2+]iplays an important role in the contraction of smooth muscle. The intravenous anesthetics ketamine,29 propofol,30 midazolam,31 and thiopental30 have been reported to inhibit smooth muscle contractile responses by reducing [Ca2+]i. Recently, we reported that ketamine attenuated acetylcholine-induced contraction in pulmonary veins.32 Because CCE is involved in the regulation of [Ca2+]iin PVSMCs, CCE may serve as a cellular target for intravenous anesthetics in PVSMCs. Therefore, we investigated the effects of intravenous anesthetics on CCE in PVSMCs. Our results indicate that clinical concentrations of ketamine33 and thiopental34 caused dose-dependent decreases in CCE in PVSMCs. Midazolam and propofol attenuated CCE only in supraclinical concentrations (midazolam: 100 μm, clinical concentration is 0.3–10 μm35; propofol: 100 μm, clinical concentration is 5–50 μm36). It has been reported that intravenous anesthetics differentially inhibit phenylephrine-induced [Ca2+]ioscillations by inhibiting CCE in PASMCs.6,37 Our results suggest that intravenous anesthetics may alter pulmonary venous tone by inhibiting CCE. To investigate the role of TK as a mechanism by which the anesthetics attenuated CCE, we performed experiments in PVSMCs after pretreatment with the TK inhibitor tyrphostin 23. Compared with TK inhibition alone, ketamine, thiopental, and midazolam no longer attenuated CCE after pretreatment with tyrphostin 23, suggesting that the TK signaling pathway is involved in the reductions in CCE caused by these anesthetics. In contrast, propofol continued to decrease CCE in the presence of tyrphostin 23, suggesting that inhibition of TK is not the primary mechanism for the propofol-induced inhibition of CCE. This result is consistent with a previous report from our laboratory that inhibition of TK is not the primary mechanism for propofol-induced inhibition of CCE in PASMCs.14 We also reported that propofol attenuated CCE via  a PKC-dependent mechanism in PASMCs.14 However, our current study demonstrated that PKC inhibition did not attenuate CCE in PVSMCs. Therefore, the propofol-induced attenuation of CCE in PVSMCs is not likely to involve PKC.
We acknowledge that results obtained from this in vitro  study can only be cautiously extrapolated to clinical practice. However, because pulmonary venous resistance is an important component of total pulmonary vascular resistance, our results provide new insight concerning the effect of intravenous anesthetics on pulmonary venous contraction.
In summary, CCE exists in PVSMCs. The TK signaling pathway positively regulates CCE, whereas the PKC and ROK signaling pathways are not involved. Ketamine, thiopental, and midazolam attenuate CCE via  a TK-dependent mechanism.
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Fig. 1. (  A  ) Representative trace depicting capacitative Ca2+entry after depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin in pulmonary venous smooth muscle cells. Extracellular Ca2+was sequentially added and removed three times.  (B  ) Summarized data showing the reproducibility of capacitative Ca2+entry. The third capacitative Ca2+entry response was decreased (*  P  < 0.05) slightly compared with the first capacitative Ca2+entry response. n = 13  .
Fig. 1. (  A  ) Representative trace depicting capacitative Ca2+entry after depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin in pulmonary venous smooth muscle cells. Extracellular Ca2+was sequentially added and removed three times. 
	(B  ) Summarized data showing the reproducibility of capacitative Ca2+entry. The third capacitative Ca2+entry response was decreased (*  P  < 0.05) slightly compared with the first capacitative Ca2+entry response. n = 13 
	.
Fig. 1. (  A  ) Representative trace depicting capacitative Ca2+entry after depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin in pulmonary venous smooth muscle cells. Extracellular Ca2+was sequentially added and removed three times.  (B  ) Summarized data showing the reproducibility of capacitative Ca2+entry. The third capacitative Ca2+entry response was decreased (*  P  < 0.05) slightly compared with the first capacitative Ca2+entry response. n = 13  .
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Fig. 2. (  A  ) Representative trace depicting the effect of the nonselective Ca2+channel blocker SKF 96365 (50 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of SKF 96365, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of the voltage-dependent Ca2+channel blocker verapamil (10 μm, n = 7) and SKF 96365 (50 μm, n = 9) on capacitative Ca2+entry, respectively. *  P  < 0.05 compared with control  .
Fig. 2. (  A  ) Representative trace depicting the effect of the nonselective Ca2+channel blocker SKF 96365 (50 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of SKF 96365, which was added to the buffer 5 min before restoring extracellular Ca2+concentration. 
	(B  ) Summarized data showing the effects of the voltage-dependent Ca2+channel blocker verapamil (10 μm, n = 7) and SKF 96365 (50 μm, n = 9) on capacitative Ca2+entry, respectively. *  P  < 0.05 compared with control 
	.
Fig. 2. (  A  ) Representative trace depicting the effect of the nonselective Ca2+channel blocker SKF 96365 (50 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of SKF 96365, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of the voltage-dependent Ca2+channel blocker verapamil (10 μm, n = 7) and SKF 96365 (50 μm, n = 9) on capacitative Ca2+entry, respectively. *  P  < 0.05 compared with control  .
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Fig. 3. (  A  ) Representative trace depicting the effect of the tyrosine kinase inhibitor tyrphostin 23 (Tyr 23: 100 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of tyrphostin 23, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of tyrphostin 23 (Tyr 23, 100 μm, n = 9), the protein kinase C activator phorbol 12-myristate 13-acetate (PMA, 1 μm, n = 16), the protein kinase C inhibitor bisindolylmaleimide I (BIS 1, 1 μm, n = 6), and the ρ-kinase inhibitor Y27632 (10 μm, n = 6) on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
Fig. 3. (  A  ) Representative trace depicting the effect of the tyrosine kinase inhibitor tyrphostin 23 (Tyr 23: 100 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of tyrphostin 23, which was added to the buffer 5 min before restoring extracellular Ca2+concentration. 
	(B  ) Summarized data showing the effects of tyrphostin 23 (Tyr 23, 100 μm, n = 9), the protein kinase C activator phorbol 12-myristate 13-acetate (PMA, 1 μm, n = 16), the protein kinase C inhibitor bisindolylmaleimide I (BIS 1, 1 μm, n = 6), and the ρ-kinase inhibitor Y27632 (10 μm, n = 6) on capacitative Ca2+entry. *  P  < 0.05 compared with control 
	.
Fig. 3. (  A  ) Representative trace depicting the effect of the tyrosine kinase inhibitor tyrphostin 23 (Tyr 23: 100 μm) on capacitative Ca2+entry. After depletion of sarcoplasmic reticulum Ca2+stores with thapsigargin, capacitative Ca2+entry was compared in the absence and presence of tyrphostin 23, which was added to the buffer 5 min before restoring extracellular Ca2+concentration.  (B  ) Summarized data showing the effects of tyrphostin 23 (Tyr 23, 100 μm, n = 9), the protein kinase C activator phorbol 12-myristate 13-acetate (PMA, 1 μm, n = 16), the protein kinase C inhibitor bisindolylmaleimide I (BIS 1, 1 μm, n = 6), and the ρ-kinase inhibitor Y27632 (10 μm, n = 6) on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
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Fig. 4. (  A  ) Summarized data showing the dose-dependent inhibitory effects of ketamine (10 μm, n = 9; 30 μm, n = 9; 100 μm, n = 8) on capacitative Ca2+entry.  (B  ) Summarized data showing the dose-dependent inhibitory effects of thiopental (30 μm, n = 9; 100 μm, n = 10) on capacitative Ca2+entry. However, 10 μm thiopental had no effect on capacitative Ca2+entry (n = 9). *  P  < 0.05 compared with control  .
Fig. 4. (  A  ) Summarized data showing the dose-dependent inhibitory effects of ketamine (10 μm, n = 9; 30 μm, n = 9; 100 μm, n = 8) on capacitative Ca2+entry. 
	(B  ) Summarized data showing the dose-dependent inhibitory effects of thiopental (30 μm, n = 9; 100 μm, n = 10) on capacitative Ca2+entry. However, 10 μm thiopental had no effect on capacitative Ca2+entry (n = 9). *  P  < 0.05 compared with control 
	.
Fig. 4. (  A  ) Summarized data showing the dose-dependent inhibitory effects of ketamine (10 μm, n = 9; 30 μm, n = 9; 100 μm, n = 8) on capacitative Ca2+entry.  (B  ) Summarized data showing the dose-dependent inhibitory effects of thiopental (30 μm, n = 9; 100 μm, n = 10) on capacitative Ca2+entry. However, 10 μm thiopental had no effect on capacitative Ca2+entry (n = 9). *  P  < 0.05 compared with control  .
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Fig. 5. (  A  ) Summarized data showing the dose-dependent inhibitory effects of midazolam (30 μm, n = 6; 100 μm, n = 9) on capacitative Ca2+entry. However, 10 μm midazolam had no effect on capacitative Ca2+entry (n = 6).  (B  ) Summarized data showing the inhibitory effects of 100 μm propofol on capacitative Ca2+entry (n = 8). However, lower concentrations of propofol (10 μm, n = 6; 30 μm, n = 7) had no effect on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
Fig. 5. (  A  ) Summarized data showing the dose-dependent inhibitory effects of midazolam (30 μm, n = 6; 100 μm, n = 9) on capacitative Ca2+entry. However, 10 μm midazolam had no effect on capacitative Ca2+entry (n = 6). 
	(B  ) Summarized data showing the inhibitory effects of 100 μm propofol on capacitative Ca2+entry (n = 8). However, lower concentrations of propofol (10 μm, n = 6; 30 μm, n = 7) had no effect on capacitative Ca2+entry. *  P  < 0.05 compared with control 
	.
Fig. 5. (  A  ) Summarized data showing the dose-dependent inhibitory effects of midazolam (30 μm, n = 6; 100 μm, n = 9) on capacitative Ca2+entry. However, 10 μm midazolam had no effect on capacitative Ca2+entry (n = 6).  (B  ) Summarized data showing the inhibitory effects of 100 μm propofol on capacitative Ca2+entry (n = 8). However, lower concentrations of propofol (10 μm, n = 6; 30 μm, n = 7) had no effect on capacitative Ca2+entry. *  P  < 0.05 compared with control  .
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Fig. 6. Summarized data showing the effects of the intravenous anesthetics on capacitative Ca2+entry in the presence of tyrphostin 23 (Tyr 23). In the presence of Tyr 23, ketamine (100 μm, n = 6), thiopental (100 μm, n = 6), and midazolam (100 μm, n = 6) no longer had an inhibitory effect on capacitative Ca2+entry. However, propofol (100 μm, n = 6) continued to decrease capacitative Ca2+entry after pretreatment with Tyr 23. *  P  < 0.05 compared with Tyr23 alone  .
Fig. 6. Summarized data showing the effects of the intravenous anesthetics on capacitative Ca2+entry in the presence of tyrphostin 23 (Tyr 23). In the presence of Tyr 23, ketamine (100 μm, n = 6), thiopental (100 μm, n = 6), and midazolam (100 μm, n = 6) no longer had an inhibitory effect on capacitative Ca2+entry. However, propofol (100 μm, n = 6) continued to decrease capacitative Ca2+entry after pretreatment with Tyr 23. *  P  < 0.05 compared with Tyr23 alone 
	.
Fig. 6. Summarized data showing the effects of the intravenous anesthetics on capacitative Ca2+entry in the presence of tyrphostin 23 (Tyr 23). In the presence of Tyr 23, ketamine (100 μm, n = 6), thiopental (100 μm, n = 6), and midazolam (100 μm, n = 6) no longer had an inhibitory effect on capacitative Ca2+entry. However, propofol (100 μm, n = 6) continued to decrease capacitative Ca2+entry after pretreatment with Tyr 23. *  P  < 0.05 compared with Tyr23 alone  .
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